模拟牲畜采食对高寒草甸土壤潜在氧化亚氮排放的影响

doi:10.11733/j.issn.1007-0435.2024.09.013

草地学报 ›› 2024, Vol. 32 ›› Issue (9): 2794-2802.DOI: 10.11733/j.issn.1007-0435.2024.09.013

• 研究论文 • 上一篇

模拟牲畜采食对高寒草甸土壤潜在氧化亚氮排放的影响

吴晓芬^1,2, 陈有超^1,2, 蒋文婷^1,2, 沈玉叶^1,2, 蔡延江^1,2

1. 浙江农林大学省部共建亚热带森林培育国家重点实验室, 浙江杭州 311300;
2. 浙江农林大学环境与资源学院/碳中和学院, 浙江杭州 311300

收稿日期:2024-03-15 修回日期:2024-05-29 发布日期:2024-10-09
通讯作者: 陈有超,E-mail:chenyouchao@zafu.edu.cn
作者简介:吴晓芬(1998-),女,汉族,浙江庆元人,硕士研究生,主要从事土壤学研究,E-mail:xiaofenwu993@163.com
基金资助:
国家自然科学基金(41877085，32371844)资助

The Response of Potential N₂O Emission from an Alpine Meadow Soil to Simulated Livestock Grazing

WU Xiao-fen^1,2, CHEN You-chao^1,2, JIANG Wen-ting^1,2, SHEN Yu-ye^1,2, CAI Yan-jiang^1,2

1. State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, Zhejiang Province 311300, China;
2. College of Environment and Resources/College of Carbon Neutrality, Zhejiang A&F University, Hangzhou, Zhejiang Province 311300, China

Received:2024-03-15 Revised:2024-05-29 Published:2024-10-09

摘要/Abstract

摘要： 探明牲畜采食对土壤氧化亚氮(N₂O)排放的影响，是准确预测放牧干扰下高寒草甸生态系统温室气体变化的关键。本研究通过刈割留茬模拟牲畜不同强度采食行为(无采食，CK；轻度采食，LD：留茬高度3 cm；重度采食，HD：留茬高度＜1 cm)，利用室内培养法测定土壤潜在N₂O排放，并测定了土壤理化性质、酶活性以及硝化和反硝化功能基因丰度等指标。结果表明：LD处理对土壤潜在N₂O排放无显著影响，但HD处理显著降低了潜在N₂O排放(P＜0.05)。重度采食导致输入土壤铵态氮(NH₄⁺-N)和碳减少，降低土壤AOA-amoA,nirS和nosZI基因丰度及反硝化潜势(Denitrification potential,DP)，减弱土壤硝化和反硝化作用从而降低N₂O排放。综上，轻度采食对土壤潜在N₂O排放无显著影响，但重度采食可能通过降低土壤硝化和反硝化作用，减弱土壤潜在N₂O排放。

关键词: 高寒草甸, 硝化作用, 反硝化作用, 放牧

Abstract: Clarifying the impact of livestock defoliation on soil nitrous oxide (N₂O) emissions is pivotal for accurately predicting the alterations in greenhouse gas of the alpine meadow ecosystems under grazing disturbance. This study simulated the grazing behavior of livestock at different intensities by mowing stubble (no grazing,CK;light grazing,LD:stubble height 3 cm;heavy grazing,HD:stubble height ＜1 cm). By using incubation methods,we measured the potential N₂O emissions from the soil,and assessed soil physicochemical properties,enzyme activities,and the abundance of nitrification and denitrification functional genes. The results showed that the LD treatment had no significant effect on soil potential N₂O emissions,but the HD treatment significantly reduced potential N₂O emissions (P＜0.05). Heavy defoliation resulted in the decrease of ammonium nitrogen (NH₄⁺-N) input and carbon input,decreased the abundance of soil AOA-amoA,nirS and nosZI genes and denitrification potential (DP),weakened the nitrification and denitrification of soil,thereby reducing soil denitrification and N₂O emissions.In conclusion,light defoliation does not have a significant impact on potential N₂O emissions from alpine meadow soil,but heavy defoliation may reduce potential N₂O emissions by jointly reducing nitrification and denitrification in soil.

Key words: Alpine meadow, Nitrification, Denitrification, Grazing

中图分类号:

S812.2

吴晓芬, 陈有超, 蒋文婷, 沈玉叶, 蔡延江. 模拟牲畜采食对高寒草甸土壤潜在氧化亚氮排放的影响[J]. 草地学报, 2024, 32(9): 2794-2802.

WU Xiao-fen, CHEN You-chao, JIANG Wen-ting, SHEN Yu-ye, CAI Yan-jiang. The Response of Potential N₂O Emission from an Alpine Meadow Soil to Simulated Livestock Grazing[J]. Acta Agrestia Sinica, 2024, 32(9): 2794-2802.

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模拟牲畜采食对高寒草甸土壤潜在氧化亚氮排放的影响

The Response of Potential N₂O Emission from an Alpine Meadow Soil to Simulated Livestock Grazing

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模拟牲畜采食对高寒草甸土壤潜在氧化亚氮排放的影响

The Response of Potential N2O Emission from an Alpine Meadow Soil to Simulated Livestock Grazing

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The Response of Potential N₂O Emission from an Alpine Meadow Soil to Simulated Livestock Grazing