草地学报

草地学报 ›› 2025, Vol. 33 ›› Issue (10): 3155-3164.DOI: 10.11733/j.issn.1007-0435.2025.10.003

• 研究论文 • 上一篇    

苜蓿种子发芽特性和胚根线粒体AsA-GSH循环对外源H2O2的响应

刘昊臻, 孙守江, 徐淑涵, 张景鈜, 李淑霞, 张金青, 高雪芹, 伏兵哲   

  1. 宁夏大学林业与草业学院, 宁夏 银川 750021
  • 收稿日期:2024-12-20 修回日期:2025-01-23 发布日期:2025-10-17
  • 通讯作者: 孙守江,E-mail:shoujiangsun@nxu.edu.cn
  • 作者简介:孙守江,E-mail:shoujiangsun@nxu.edu.cn
  • 基金资助:
    国家自然基金青年科学基金（C类）（32503269）；宁夏自然科学基金（2025A1211）资助

Response of Germination Characteristics and Mitochondria AsA-GSH Cycle during Alfalfa Seed Germination to Exogenous H2O2

LIU Hao-zhen, SUN Shou-jiang, XU Shu-han, ZHANG Jing-hong, LI Shu-xia, ZHANG Jing-qing, GAO Xue-qin, FU Bing-zhe   

  1. College of Forestry and Grassland Science, Ningxia University, Yinchuan, Ningxia 750021, China
  • Received:2024-12-20 Revised:2025-01-23 Published:2025-10-17

PDF

摘要： 为探究外源H2O2对紫花苜蓿种子萌发及胚根线粒体AsA-GSH循环的影响，本试验以紫花苜蓿（Medicago sativa）种子为材料，研究了不同浓度梯度（0%，0.25%，0.5%，1%，2%）H2O2处理下种子发芽特性、幼苗生长状态后，提取胚根线粒体测定AsA-GSH循环抗氧化酶活性和抗氧化物含量的变化。结果表明，2%H2O2处理下，苜蓿种子的发芽特性和幼苗生长均被显著（P<0.05）抑制；随着处理浓度的增加，MDA含量呈现升高趋势，AsA-GSH循环中GR活性呈先上升后下降再上升的趋势；SOD、MDHAR和DHAR活性呈现先上升后下降的趋势，AsA含量呈上升而后逐渐下降的趋势；DHA和GSSH含量呈升高趋势。说明外源H2O2处理影响了苜蓿种子萌发生长进程，高浓度的H2O2抑制了苜蓿种子发芽和幼苗生长，加强抗氧化系统的运转来抵御外源H2O2造成的胁迫。

关键词: 紫花苜蓿, 外源H2O2, 线粒体, 抗氧化酶, 抗氧化物, AsA-GSH循环

Abstract: To investigate the effects of exogenous H2O2 on alfalfa（Medicago sativa）seeds germination and AsA-GSH cycle in embryonic root mitochondria， this study took alfalfa seeds as materials to study the seed germination characteristics and seedling growth status under different concentration gradients （0%， 0.25%， 0.5%， 1% and 2%） of H2O2 treatments， and then extracted embryonic root mitochondria to determine the changes in AsA-GSH cycle antioxidant enzyme activities and antioxidant contents. The results showed that germination characteristics and seedling growth of alfalfa seeds were significantly （P<0.05） inhibited under 2% H2O2 treatment. With the increase of treatment concentration， the malondialdehyde （MDA） content exhibited an increase trend， while the activity of glutathione reductase （GR） in the AsA-GSH cycle initially was increased， then decreased， and subsequently increased again. The activities of superoxide dismutase （SOD）， monodehydroascorbate reductase （MDHAR）， and dehydroascorbate reductase （DHAR） followed a pattern of increaed first and then decreasing， whereas the ascorbic acid （AsA） content increased and then decreased gradually. The levels of dehydroascorbic acid （DHA） and oxidized glutathione （GSSH） showed an increasing trend. It was indicated that exogenous H2O2 treatment affected the germination and growth process of alfalfa seeds， and the high concentration of H2O2 inhibited the germination and seedling growth of alfalfa seeds， and strengthened the operation of the antioxidant system to resist the stress caused by exogenous H2O2.

Key words: Alfalfa, Exogenous H2O2, Mitochondria, Antioxidant enzyme, Antioxidant, AsA -GSH cycle

中图分类号: