高原鼠兔干扰对高寒草甸土壤古菌群落特征的影响

doi:10.11733/j.issn.1007-0435.2025.12.012

草地学报 ›› 2025, Vol. 33 ›› Issue (12): 3994-4003.DOI: 10.11733/j.issn.1007-0435.2025.12.012

• 研究论文 • 上一篇

高原鼠兔干扰对高寒草甸土壤古菌群落特征的影响

王丝瑜^1,2,3,4, 李琳^1,2,3,4, 陈克龙^2,3,4, 章妮^2,3,4, 孙建青⁵, 史倩倩⁵

1. 青海师范大学生命科学学院, 青海西宁 810008;
2. 青海师范大学地理科学学院青海省自然地理与环境过程重点实验室, 青海西宁 810008;
3. 青海师范大学青藏高原地表过程与生态保护教育部重点实验室, 青海西宁 810008;
4. 国家林业和草原局青海湖湿地生态系统国家定位观测研究站, 青海海北 812200;
5. 青海湖国家级自然保护区管理局, 青海刚察 812300

收稿日期:2024-12-06 修回日期:2025-06-20 发布日期:2025-12-01
通讯作者: 陈克龙，E-mail：ckl7813@163.com；章妮，E-mail：1581146264@qq.com
作者简介:王丝瑜（2000-），女，汉族，陕西咸阳人，硕士研究生，主要从事湿地生态学研究，E-mail：2637286473@qq.com；
基金资助:
国家自然科学基金（42461018）；青海省科技计划（2023-ZJ-905T）资助

Effects of Plateau Pika Disturbance on Soil Archaea Community Characteristics in Alpine Meadow

WANG Si-yu^1,2,3,4, LI Lin^1,2,3,4, CHEN Ke-long^2,3,4, ZHANG Ni^2,3,4, SUN Jian-qing⁵, SHI Qian-qian⁵

1. College of Life Sciences, Qinghai Normal University, Xining, Qinghai Province 810008, China;
2. Qinghai Province Key Laboratory of Physical Geography and Environmental Process, College of Geographical Science, Qinghai Normal University, Xining, Qinghai Province 810008, China;
3. Key Laboratory of Tibetan Plateau Land Surface Processes and Ecological Conservation (Ministry of Education), Qinghai Normal University, Xining, Qinghai Province 810008, China;
4. National Positioning Observation and Research Station of Qinghai Lake Wetland Ecosystem in Qinghai, National Forestry and Grassland Administration, Haibei, Qinghai Province 812200, China;
5. Qinghai Lake National Nature Reserve Administration, Gangcha, Qinghai Province 812300, China

Received:2024-12-06 Revised:2025-06-20 Published:2025-12-01

摘要/Abstract

摘要： 高原鼠兔作为青藏高原高寒草甸生态系统中的特有哺乳动物，其干扰可能加速高寒草甸退化，而高寒草甸退化对土壤古菌群落有显著影响。本研究以高原鼠兔干扰下的表层土壤（0~10 cm，ST₁）与亚表层土壤（10~20 cm，ST₂）作为研究对象，基于古菌16S rRNA进行高通量测序，对高原鼠兔干扰下的土壤古菌群落特征变化进行研究。结果表明：不同处理下土壤古菌的优势菌门均为奇古菌门（Thaumarchaeota），优势菌属均为亚硝化球菌属（Candidatus Nitrososphaera）；与自然状态相比，高原鼠兔干扰下土壤古菌群落的表层土壤多样性上升，亚表层土壤多样性下降；LEfSe分析发现高原鼠兔干扰增加亚表层土壤中亚硝化球菌属、海洋异养古菌类群（Marine Group II）及热原体纲（Thermoplasmata）3个与氮循环相关菌群的相对丰度。总而言之，高原鼠兔活动影响了高寒草甸土壤古菌的群落结构，且对表层土壤的影响较明显。

关键词: 高通量测序, 青藏高原, 奇古菌门, 亚硝化球菌属

Abstract: As a unique mammal in the alpine meadow ecosystem of the Qinghai-Tibet Plateau， the disturbance caused by plateau pikas （Ochotona curzoniae） may accelerate alpine meadow degradation The degradation of the alpine meadow has a significant affects on the soil archaeal communities. In this study， we investigated the characteristics of soil archaeal communities under pika disturbance by analyzing surface soil （0-10 cm，ST₁） and subsurface soil （10-20 cm，ST₂） through high-throughput sequencing of archaeal 16S rRNA. The results revealed that Thaumarchaeota remained the dominant archaeal phylum across all treatments， with Candidatus Nitrososphaera as the predominant genus. Compared to natural conditions， pika disturbance increased archaeal diversity in surface soil but decreased it in subsurface soil. LEfSe analysis demonstrated that pika activity significantly enhanced the abundance of three nitrogen-cycling taxa in subsurface soil： Candidatus Nitrososphaera， Marine Group II， and Thermoplasmata. In conclusion， plateau pika activities alter the structure of soil archaeal communities in alpine meadows， and the impact on the surface soil was more obvious.

Key words: High-throughput sequencing, Tibetan Plateau, Thaumarchaeota, Candidatus Nitrososphaera

中图分类号:

S812.2

王丝瑜, 李琳, 陈克龙, 章妮, 孙建青, 史倩倩. 高原鼠兔干扰对高寒草甸土壤古菌群落特征的影响[J]. 草地学报, 2025, 33(12): 3994-4003.

WANG Si-yu, LI Lin, CHEN Ke-long, ZHANG Ni, SUN Jian-qing, SHI Qian-qian. Effects of Plateau Pika Disturbance on Soil Archaea Community Characteristics in Alpine Meadow[J]. Acta Agrestia Sinica, 2025, 33(12): 3994-4003.

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高原鼠兔干扰对高寒草甸土壤古菌群落特征的影响

Effects of Plateau Pika Disturbance on Soil Archaea Community Characteristics in Alpine Meadow

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