副地衣芽孢杆菌对多年生黑麦草非生物胁迫抗性的生理调控机制

doi:10.11733/j.issn.1007-0435.2026.07.009

草地学报 ›› 2026, Vol. 34 ›› Issue (7): 2440-2449.DOI: 10.11733/j.issn.1007-0435.2026.07.009

• 研究论文 • 上一篇

副地衣芽孢杆菌对多年生黑麦草非生物胁迫抗性的生理调控机制

于乔¹, 王鹤潼¹, 杨万强¹, 孟庆娴¹, 张宇婧², 普布卓玛³, 呼天明¹, 付娟娟¹

1. 西北农林科技大学草业与草原学院, 陕西杨凌 712100;
2. 巴彦淖尔市耕地质量监测保护中心, 内蒙古巴彦淖尔 015000;
3. 西藏自治区农牧科学院草业科学研究所, 西藏拉萨 850000

收稿日期:2025-11-28 修回日期:2026-01-28 发布日期:2026-07-02
通讯作者: 付娟娟，E-mail:fujuanjuan@nwafu.edu.cn
作者简介:于乔（2001-），男，汉族，吉林四平人，硕士研究生，主要从事植物与微生物互作研究，E-mail:2229300337@qq.com
基金资助:
西藏自治区重点研发及转化项目-二次科考项目（XZ202502ZY0050）资助

Physiological Mechanisms Underlying the Modulation of Abiotic Stress Tolerance in Perennial Ryegrass by Bacillus paralicheniformis

YU Qiao¹, WANG He-tong¹, YANG Wan-qiang¹, MENG Qing-xian¹, ZHANG Yu-jing², PUBU Zhuoma³, HU Tian-ming¹, FU Juan-juan¹

1. College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
2. Bayannur Cultivated Land Quality Monitoring and Protection Center, Bayannur, Inner Mongolia 015000, China;
3. Institute of Grassland Science, Xizang Academy of Agricultural and Animal Husbandry Sciences, Lhasa, Xizang 850000, China

Received:2025-11-28 Revised:2026-01-28 Published:2026-07-02

摘要/Abstract

摘要： 为探究植物生长促生菌对多年生黑麦草（Lolium perenne L.）抗旱、抗寒及耐盐碱能力的生理调控机制，本研究以副地衣芽孢杆菌（Bacillus paralicheniformis， NCBI ID： PRJNA1402526）菌株为研究对象，采用盆栽接菌试验，设置干旱、低温、盐碱三种非生物胁迫处理，分析根际接种该菌株对多年生黑麦草生长指标、生理特性及根系形态的影响。发现接种副地衣芽孢杆菌显著缓解了非生物胁迫对多年生黑麦草生长的抑制。与未接种相比，接种处理的株高、地上部和地下部生物量均显著提高，叶片相对电导率和丙二醛含量显著降低，超氧化物歧化酶、过氧化物酶、过氧化氢酶活性及脯氨酸、可溶性糖含量显著上升，且根系形态构型得到显著改善（P<0.05）。综上，接种副地衣芽孢杆菌可通过协同增强多年生黑麦草的抗氧化防御能力、渗透调节功能及根系构型，从而显著提高其对干旱、低温和盐碱胁迫的抗性。

关键词: 副地衣芽孢杆菌, 多年生黑麦草, 非生物胁迫, 抗氧化酶, 渗透调节, 根系形态

Abstract: To investigate the physiological regulation mechanisms of plant growth-promoting bacteria on the drought， cold， and saline-alkali stress tolerance of perennial ryegrass （Lolium perenne L.）， this study used Bacillus paralicheniformis （NCBI ID： PRJNA1402526） as the test strain. A pot inoculation experiment was conducted under three abiotic stress conditions-drought， cold， and saline-alkali stress—to analyze the effects of rhizosphere inoculation on the growth indices， physiological characteristics， and root morphology of perennial ryegrass. The results showed that inoculation with B. paralicheniformis significantly alleviated the growth inhibition caused by abiotic stresses. Compared to the non-inoculated control， the inoculated plants exhibited significantly increased plant height， above-ground and below-ground biomass， along with markedly reduced leaf relative conductivity and malondialdehyde content. Additionally， the activities of superoxide dismutase， peroxidase， and catalase， as well as the contents of proline and soluble sugars， were significantly enhanced （P<0.05）. Moreover， root system architecture was notably improved. In conclusion， inoculation with B. paralicheniformis can significantly enhance the resistance of perennial ryegrass to drought， cold， and saline-alkali stresses by synergistically strengthening its antioxidant defense capacity， osmoregulatory function， and root morphological configuration.

Key words: Bacillus paralicheniformis, Perennial ryegrass, Abiotic stress, Antioxidant enzymes, Osmotic regulation, Root morphology

中图分类号:

S543+.6

于乔, 王鹤潼, 杨万强, 孟庆娴, 张宇婧, 普布卓玛, 呼天明, 付娟娟. 副地衣芽孢杆菌对多年生黑麦草非生物胁迫抗性的生理调控机制[J]. 草地学报, 2026, 34(7): 2440-2449.

YU Qiao, WANG He-tong, YANG Wan-qiang, MENG Qing-xian, ZHANG Yu-jing, PUBU Zhuoma, HU Tian-ming, FU Juan-juan. Physiological Mechanisms Underlying the Modulation of Abiotic Stress Tolerance in Perennial Ryegrass by Bacillus paralicheniformis[J]. Acta Agrestia Sinica, 2026, 34(7): 2440-2449.

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副地衣芽孢杆菌对多年生黑麦草非生物胁迫抗性的生理调控机制

Physiological Mechanisms Underlying the Modulation of Abiotic Stress Tolerance in Perennial Ryegrass by Bacillus paralicheniformis

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