结缕草属植物种间关系和遗传多样性的SSR标记分析

doi:10.11733/j.issn.1007-0435.2008.06.003

›› 2008, Vol. 16 ›› Issue (6): 552-558.DOI: 10.11733/j.issn.1007-0435.2008.06.003

结缕草属植物种间关系和遗传多样性的SSR标记分析

郭海林^1,2, 刘建秀², 周志芳², 宣继萍²

1. 南京农业大学园艺学院, 南京, 210095;
2. 江苏省中国科学院植物研究所, 南京中山植物园, 南京, 210014

收稿日期:2008-09-18 修回日期:2008-10-07 出版日期:2008-12-15 发布日期:2008-12-15
通讯作者: 刘建秀,E-mail:turfunit@yahoo.com.cn
作者简介:郭海林(1975- ),女,内蒙古乌盟人,助理研究员,在职博士生,主要从事暖季型草坪草的种质资源研究及育种工作,E-mail:ghlnmg@sina.com.
基金资助:
国家自然科学基金项目(30571307)

Interspecific Relationship and Genetic Diversity of Zoysiagrass Revealed by SSR Markers

GUO Hai-lin^1,2, LIU Jian-xiu², ZHOU Zhi-fang², XUAN Ji-ping²

1. College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China;
2. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, Jiangsu Province 210014, China

Received:2008-09-18 Revised:2008-10-07 Online:2008-12-15 Published:2008-12-15

摘要/Abstract

摘要： 利用SSR分子标记技术对结缕草属(Zoysia Willd.)5种、1变种共96份种质材料进行遗传多样性分析,结果表明:(1)结缕草属种质间存在丰富的遗传多样性,29对SSR引物共扩增出282条清晰谱带,其中多态性条带272条,多态性比率(PPB)为96.45%,Nei’s基因多样性指数(H)为0.2203,Shannon信息指数(I)为0.3504。(2)应用Nei—Li相似系数法估算了96份材料间的遗传相似系数(GS),范围在0.5922~0.9362,平均为0.7811,在不同种之间,结缕草(Z.japonica Steud.)与中华结缕草(Z.sinica Hance.)、沟叶结缕草(Z.matrella(L.)Merr.)与细叶结缕草(Z.tenuifloia Willd.ex Trin.)中的遗传相似系数较高,大穗结缕草(Z.macrostachya Franch.)Z010、长花结缕草(Z.sinica var.nipponica)Z122与其它结缕草的遗传相似系数均相对较低。结缕草、中华结缕草和沟叶结缕草的种内遗传变异较大,GS分别为0.5922~0.9362,0.6879~0.9078和0.6738~0.8582。(3)通过分子系统聚类法,将96份种源分为6个大类群,其中第一大类主要包括结缕草、中华结缕草和少量沟叶结缕草,占所有材料的90.6%。沟叶结缕草和细叶结缕草聚成一小类,大穗结缕草Z010、长花结缕草Z122、沟叶结缕草(Z.matrella)Z095和结缕草(Z.japonica)Z115聚为一类。从亚群来看,基本上结缕草与中华结缕草交叉相聚,沟叶结缕草与细叶结缕草交叉相聚,沟叶结缕草与结缕草、中华结缕草也有交叉聚类现象。本研究明确了结缕草属植物不同种质间亲缘关系及遗传多样性、部分种质的种类归属,还为其进一步研究与利用提供了分子水平的依据。

关键词: 结缕草属, 种质资源, 种间关系, 遗传多样性, SSR

Abstract: The genetic diversity and interspecific relationship of 96 germplasms of Zoysia Willd.belonging to 5 species and 1 mutation were analyzed using SSR(Simple Sequence Repeat) markers.The results showed that:(1)Total 282 bands were detected by 29 pairs of SSR primers,and 272 of 282 were polymorphic with PPB(Percentage of Polymorphic Bands) at 96.45%,the Nei’s gene diversity index(H) was 0.2203,and the Shannon diversity index(I) was 0.3504,indicating a high level of genetic diversity in zoysiagrass.(2) The GS(genetic similarity) among 96 Zoysia germplasms ranged from 0.5922 to 0.9362 with the average of 0.7811.GS between Z.japonica and Z.sinica and between Z.matrella and Z.tenuifolia were relatively high,both Z.machrostachya(Z010) and Z.sinica.var.nipponica(Z122) had a relatively lower GS than other species.The range of GS within species of Z.japonica,Z.sinica,and Z.matrella were 0.5922~0.9362,0.6879~0.9078,and 0.6738~0.8582,respectively.(3) Genetic distance and relationship among 96 Zoysia germplasms were showed clearly in the cluster dendrogram.The 96 Zoysia germplasms were classified into 6 major groups by cluster analysis.Group I included Z.Japonica,Z.sinica,and some accessions of Z.matrella,accounted for 90.6% of all germplasms.Some accessions of Z.matrella and Z.tenuifolia form one group.Z.machrostachya(Z010),Z.sinic var.nipponica(Z122),Z. matrella(Z095),and Z.sinica(Z115) form one group by itself,respectively.The cluster results of some secondary groups showed that most accessions of Z.japonica and Z.Sinica were clustered to one group,whereas,Z.matrella and Z.tenuifolia were one group,and some accessions of Z.japonica,Z.Sinica and Z.matrella were one group.In this research,the diversity and relationship among 96 germplasms were displayed;meanwhile,a preliminary discussion was made on the ownership of part accessions.We consider the findings could provide a scientific basis at the molecular level for future study and application of zoysiagrass germplasms.

Key words: Zoysia Willd., Germplasm resources, Interspecific relationship, Genetic diversity, Simple Sequence Repeat

中图分类号:

Q943.2

郭海林, 刘建秀, 周志芳, 宣继萍. 结缕草属植物种间关系和遗传多样性的SSR标记分析[J]. , 2008, 16(6): 552-558.

GUO Hai-lin, LIU Jian-xiu, ZHOU Zhi-fang, XUAN Ji-ping. Interspecific Relationship and Genetic Diversity of Zoysiagrass Revealed by SSR Markers[J]. , 2008, 16(6): 552-558.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2008.06.003

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2008/V16/I6/552

参考文献

[1] 李亚,谢晓金,宣继萍.中国结缕草属 (Zoysia spp.) 植物抗寒性评价[J].草地学报,2003,11(3):240-245
[2] 李亚,耿蕾,刘建秀.中国结缕草属植物抗盐性评价[J].草地学报,2004,12(1):8-11
[3] Engelke M C,Anderson S.Zoysiagrasses (Zoysia spp.)[A].In:Michael D C,Ronny R D.eds.Turfgrass biology,genetics and breeding[M].New Jersey:John Wiley and Sons,Inc.2002,271-285
[4] 董厚德,宫莉君.中国结缕草生态学及其资源开发与应用[M].北京:中国林业出版社,2001
[5] 胡化广,刘建秀.结缕草属植物抗旱形态机理[J].草地学报,2008,16(1):94-96
[6] Anderson S J.Taxonomy of Zoysia (Poaceae):morphological and molecular variation[D] :[Ph.D.dissertation].College Station,TX:Texas A&M University,2000
[7] Yaneshita M,Nagasawa R,Engelke M C.Genetic variation and interspecific hybridization among natural populations of zoysiagrasses detected by RFLP analyses of chloroplast and nuclear DNA[J].Genes and Genetic System,1997,72(4):173-179
[8] 周志芳,宣继萍,刘建秀.结缕草属植物种间关系的等位酶研究[J].草地学报,2007,15(6):577-581
[9] 刘建秀,贺善安,陈守良.华东地区结缕草属植物形态类型及坪用评价[J].草地学报,1997,1(51):43-47
[10] 李亚,凌萍萍,刘建秀.中国结缕草属植物(Zoysia spp.)地上部分形态类型多样性[J].植物资源与环境学报,2002,11(4):33-39
[11] 李亚,凌萍萍,刘建秀,等.中国结缕草属植物(Zoysia spp.)地下部分分布和形态类型的多样性[J].植物资源与环境学报,2002,11 (2):39-44
[12] Weng J H,Liao T S,Chen Y C.Distribution and morphological variation of Zoysia spp.grown in Taiwan[J].Journal of the Agricultural Association of China,1995,169:44-54
[13] 翁仁宪,陈奕君,廖天赐.结缕草酯酶同工酶的研究[J].中国草地,1996(1):56-66
[14] Weng J H.Genetic variation of Zoysia in Taiwan as analyzed by isozyme patterns and salinity tolerance[J].Plant Production Science,2002,5:236-241
[15] Kitamura F.The climate of Japan and its surrounding areas and the distribution and classification of zoysiagrasses[J].International Turfgrass Society Research Journal,1989,(6):17-21
[16] Yang G M,Ahn B J,Choi J S.Identification of native zoysiagrass (Zoysia spp.) using morphological characteristics and esterase isozymes[J].Journal Korea Society Horticultural Science,1995,36(2):240-247
[17] Choi J S,Ahn B J,Yang G M.Distribution of zoysiagrass (Zoysia spp.) in the south and west coastal regions of Korea and classification using morphological characteristics[J].Journal Korea Society Horticultural Science,1997,38(4):399-407
[18] 郭海林,刘建秀.三种暖季型草坪草的开花习性及自交结实率的研究[J].草原与草坪,2001,增刊:45-47
[19] Hong K H,Yeam D Y.Studies on interspecific hybridization in Korean lawngrass (Zoysia spp.)[J].Juornal of the Korean Society for Horticultural Science (Korean R.),1985,26(2):169-178
[20] Lee G J,Yoo K,Kim K S.Comparative salt tolerance study in Koreanlawngrass.II.Interspecific comparison among eight zoysiagrass (Zoysia spp.)[J].Journal Korea Society Horticultural Science,1994,35 (2):178-185
[21] Forbes I.Chromosome numbers and hybrids in Zoysia.Agronomy Journal[J] ,1952,44:194-199
[22] Yaneshita M,Kaneko S,Sasakuma T.Alleotetraploid of Zoysia species with 2n=40 based on a RFLP genetic map[J].Theoretical and Applied Genetics,1999,98(5):751-756
[23] Choi J S,Yang G M.PCR conditions for effective identification of Korean native zoysiagrass (Zoysia spp.) species by DNA polymorphism[J].Journal Korea Society Horticultural Science,1996,37(1):166 -170
[24] Choi J S,Ahn B J,Yang G M.Classification of zoysiagrass (Zoysia spp.) native to the southwest coastal of Korea using RAPDS[J].Journal Korea Society Horticultural Science,1997,38(6):789 -795
[25] Weng J H,Fan M J,Lin C Y,et al.Genetic variation of Zoysia as revealed by random amplified polymorphic DNA (RAPD) and isozyme pattern[J].Plant Production Science,2007,10(1):80-85
[26] 金洪.中国结缕草(Zoysia japonica steud.) 遗传多样性研究[D] :[博士学位论文].北京:北京林业大学,2004
[27] 黄占兵.结缕草遗传多样性研究[D].硕士学位论文.内蒙古农业大学,2004
[28] 彭锁堂,庄杰云,颜启传.我国主要杂交水稻组合及其亲本SSR标记和纯度鉴定[J].中国水稻科学,2003,17(1):1-5
[29] Kubik C,Sawkins M,Meyer W A,et al.Genetic diversity in seven perennial ryegrass (Lolium perenne L.) cultivars based on SSR markers[J].Crop Science,2001,41:1565-1572
[30] Dayanandan S,Bawa K S,Kesseli R.Conservation of microsatellites among tropical tree (Leguminosae)[J].American Journal of Botany,1997,84 (12):658-1663
[31] Primmer C R,Ellegren H.Patterns of molecular evolution in avian microsatellites[J].Molecular Biology and Eveluation,l998,15(8):997-1008
[32] Cuadrado A,Schwarzacher T.The chromosomal organization of simple sequence repeats in wheat and rye genomes[J].Chromosoma,1998,107(8):587-594
[33] 郭海林,刘建秀,高鹤,等.结缕草属优良品系SSR指纹图谱的构建[J].草业学报,2007,16(2):53-59
[34] Yeh F C,Yang R C,Boyle T B J.POPGENE,the user-friendly shareware for population genetic analysis,1997,http://www.ualberta.ca/～fyeh/fyeh/

结缕草属植物种间关系和遗传多样性的SSR标记分析

Interspecific Relationship and Genetic Diversity of Zoysiagrass Revealed by SSR Markers

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	马骢毓, 韩重阳, 马赛男, 李旭旭, 蔡家邦, 汪阳, 张新全, 聂刚. 基于SSR分子标记的13个白三叶(Trifolium repens L.)品种指纹图谱构建[J]. 草地学报, 2021, 29(9): 1892-1899.
[2]	王子玥, 刘曼, 刘凌云, 常智慧. 苇状羊茅EST-SSR标记在草甸羊茅和多年生黑麦草的通用分析[J]. 草地学报, 2021, 29(9): 1900-1908.
[3]	罗铭欣, 刘凤民, 陈纪言, 崔均涛, 张伟丽. ⁶⁰Coγ辐射柱花草M₃代的农艺性状及遗传多样性分析[J]. 草地学报, 2021, 29(9): 1992-2000.
[4]	梁小玉, 季杨, 胡远彬, 易军, 白史且, 张靓, 张新全. 菊苣EST-SSR分子标记开发及通用性分析[J]. 草地学报, 2021, 29(9): 2081-2090.
[5]	叶刚, 张笑笑, 陈静波, 李建建, 李玲, 刘建秀, 郭爱桂, 郭海林. 结缕草属植物杂交后代形态特征和SRAP分子标记鉴定[J]. 草地学报, 2021, 29(7): 1406-1415.
[6]	刘凌云, 范希峰, 滕珂, 岳跃森, 常智慧, 武菊英. 苔草属植物遗传多样性研究进展[J]. 草地学报, 2021, 29(6): 1148-1157.
[7]	陈志祥, 罗小燕, 李拴林, 吴如月, 王文强, 丁西朋. 基于SSR标记的木豆种质资源遗传多样性与群体结构分析[J]. 草地学报, 2021, 29(5): 904-911.
[8]	安金婵, 常丹, 白史且, 陈智华, 鄢家俊, 张建波, 李达旭, 游明鸿, 毛德才, 王艳, 杨金平, 李英主, 邱文静. 基于形态特征和SSR标记的斑茅繁殖策略的研究[J]. 草地学报, 2021, 29(4): 813-820.
[9]	张鹏, 李毅, 单立山, 解婷婷. 降水变化对混生红砂-珍珠生物量及分配的影响[J]. 草地学报, 2021, 29(3): 478-487.
[10]	梁国玲, 刘文辉, 马祥. 590份皮燕麦种质资源穗部性状遗传多样性分析[J]. 草地学报, 2021, 29(3): 495-503.
[11]	张琦, 魏臻武, 闫天芳, 耿小丽. 燕麦种质资源农艺性状遗传多样性的鉴定评价[J]. 草地学报, 2021, 29(2): 309-316.
[12]	安明珠, 韩博, 姜华, 段新慧, 文亦芾, 周凯. 鸭茅物种起源与系统发育分析研究进展[J]. 草地学报, 2021, 29(12): 2637-2644.
[13]	李思达, 刘文辉, 张永超, 秦燕, 李文. 青海扁茎早熟禾混播草地生产性能与种间关系的研究[J]. 草地学报, 2021, 29(12): 2827-2837.
[14]	吴国芳, 于卓, 于肖夏, 杨东升, 卢倩倩, 李景伟, 李佳奇. 高丹草低氢氰酸含量性状主效QTL PA7-2的精细定位[J]. 草地学报, 2021, 29(10): 2149-2157.
[15]	黄春琼, 张子雄, 王文强, 刘国道. 柱花草花粉不育基因连锁的SCAR标记研究[J]. 草地学报, 2020, 28(5): 1478-1483.