关键词: 关键字:针茅, RAPD, 种质资源, 聚类分析

Abstract: Stipa baicalensis Roshev., S.grandis P. Smirn, and S.krylovii Roshev are constructive species of the Inner Mongolia steppes. They are not only the main resources of forage in Inner Mongolia, but also the natural ecological defense of the Beijing-Tianjin area, boasting very important economical and environmental values. Studies on these Stipa species have been reported both in the domestic and overseas journals, focusing mainly on the grasses geographical distribution, and their biological and physiological characteristics. With the development of molecular biology techniques, studies on the molecular biology of Stipa species have drawn the attention of many researchers. Though analysis of the genetic similarities and genetic structure of one species of Stipa have been documented, research work on the genetic relationship between different Stipa species are still lacking. In the present study, 14 populations of three Stipa species from the middle and eastern Inner Mongolia steppes were analyzed with RAPD markers. We obtained 223 bands from the 16 primers, in which 184 bands were polymorphic. The rate of polymorphism was 82.51%. There were 14 samples to show the obvious similarity among the species, and populations within the same species were initially clustered together. We obtained the special RAPD markers within the populations of the same species.

Key words: Stipa, RAPD, Germplasm, Cluster analysis