草地学报

›› 2004, Vol. 12 ›› Issue (4): 273-275,280.DOI: 10.11733/j.issn.1007-0435.2004.04.004

• 研究论文 • 上一篇    下一篇

Bar基因转化草原1号苜蓿的研究

刘艳芝, 王玉民, 刘莉, 隋松兵, 李望丰, 董英山*   

  1. 吉林省农业科学院生物技术研究中心, 吉林, 公主岭, 136100
  • 收稿日期:2003-09-10 修回日期:2004-03-09 出版日期:2004-11-15 发布日期:2004-11-15
  • 通讯作者: 董英山
  • 作者简介:刘艳芝(1964- ),女,副研究员,主要从事植物遗传转化工作,E-mail:ysdong@263.net;董英山,通讯作者Authorforcorrespondence
  • 基金资助:
    国家植物转基因中试基地资助课题

Agrobacterium-Mediated Transformation of Alfalfa Variety,Caoyuan No.1,by Bar Gene

LIU Yan-zhi, WANG Yu-min, LIU Li, SUI Song-bing, LI Wang-feng, DONG Ying-shan   

  1. Biotechnology Research Center, Jilin Academy of Agri culture Sciences, Gongzhuling, Jilin Province, 136100, China
  • Received:2003-09-10 Revised:2004-03-09 Online:2004-11-15 Published:2004-11-15

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摘要： 利用农杆菌介导法将抗草丁膦的Bar基因导入草原1号苜蓿,经叶片筛选试验证实转基因植株对除草剂Basta具有抗性,通过多聚白酶连式反映检测,初步证实目的基因已转入到苜蓿基因组中.

关键词: 生物技术, 苜蓿, 农杆菌介导, 遗传转化

Abstract: Bar gene coding Glufosinate resistance is transformed into the alfalfa variety, Caoyuan No.1, mediated by Agrobacterium tumefaciens. The leaves of transgenic plants after being screened and tested are proved to be resistant to Glufosinate. Furthermore, PCR analysis shows that bar gene has been transformed into Alfalfa.

Key words: Biotechnology, Alfalfa, Agrobacterium tumefaciens, Genetic transformation

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