QTL Analysis of Traits Relating to Cold Resistance of Zoysia japonica

doi:10.11733/j.issn.1007-0435.2010.05.017

›› 2010, Vol. 18 ›› Issue (5): 703-707.DOI: 10.11733/j.issn.1007-0435.2010.05.017

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QTL Analysis of Traits Relating to Cold Resistance of Zoysia japonica

DING Cheng-long¹, LIU Ying², SHEN Yi-xin¹, GU Hong-ru¹

1. Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing Jiangsu Province 210014, China;
2. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing Jiangsu Province 210095, China

Received:2010-03-08 Revised:2010-09-14 Online:2010-10-15 Published:2010-10-15

日本结缕草抗寒相关性状的QTL分析

丁成龙¹, 刘颖², 许能祥¹, 顾洪如¹

1. 江苏省农业科学院畜牧研究所, 江苏, 南京, 210014;
2. 南京农业大学动物科技学院, 江苏, 南京, 210095

通讯作者: 顾洪如,E-mail:guhongru@jaas.ac.cn
作者简介:丁成龙(1969- ),男,江苏滨海人,研究员,博士,主要从事牧草新品种选育及栽培利用研究,E-mail:dingcl@jaas.ac.cn
基金资助:
国家自然科学基金(30771372);国家科技支撑计划课题(2008BADB3B10);江苏省科技支撑计划课题(BE3009300)资助

Abstract

Abstract: To find quantitative trait loci(QTLs) relating to cold resistance of Zoysia japonica,two different ecotypes(Muroran and Tawarayama Kita) and a pseudo-testcross F₁ population(86 individuals derived by crossing ’Zoysia japonica cv.Muroran’ with ’Zoysia japonica cv.Tawarayama Kita’) were used for mapping and QTL analysis.A genetic linkage map with 447 SSR markers was constructed for zoysiagrass(Zoysia japonica Steud.).The semi lethal temperature(LT₅₀),soluble protein,soluble sugar content and SOD activity of leaves of F₁ population were analyzed.Results indicate a significant negative correlation between the leaf LT₅₀ and the soluble protein content of F₁ population(P<0.05).Also very significant negative correlations were observed between the leaf LT₅₀ and the soluble sugar content as well as the SOD activity of F₁ population(P<0.01) under the treatment of 0℃ for 2 days.Three QTLs,located on tenth,thirteenth and seventeenth linkage group respectively,relating to the soluble protein,the soluble sugar content and the SOD activity were detected.The score of LOD ranged from 2.19 to 2.42,and the phenotypic variation explained by individual QTL varied from 13.3% to 13.8%.

Key words: Zoysia japonica, Cold resistance, Soluble protein content, Soluble sugar content, SOD activity, QTL analysis

摘要： 为寻找与结缕草(Zoysia japonica steud.)抗寒性状相关的数量性状位点(QTLs),利用生态条件具明显差异的2个日本结缕草品系室兰(Zoysia japonsic cv. Muroran)和俵山北(Zoysia japonsic cv. Tawarayama Kita)及其假测交产生的F₁代86个个体为材料,研究其在人工低温胁迫条件下叶片的半致死温度(LT₅₀)及可溶性糖、可溶性蛋白含量及过氧化物歧化酶(SOD)活性的变化,并以447个SSR标记构建的日本结缕草遗传连锁图谱为基础,对抗寒相关的性状可溶性糖、可溶性蛋白含量及SOD活性进行了QTL定位分析。结果表明:F₁群体的可溶性蛋白含量与叶片LT₅₀呈显著负相关(P<0.05),可溶性糖含量及SOD活性与叶片LT₅₀呈极显著负相关(P<0.01)。分别定位到与叶片可溶性糖、可溶性蛋白含量和SOD活性相关的QTL各1个,分布于3个连锁群上,QTL的LOD值介于2.19~2.42之间,单个QTL可解释性状表型变异的范围在13.3%~13.8%。

关键词: 日本结缕草, 抗寒性, 可溶性蛋白含量, 可溶性糖含量, SOD活性, QTL分析

CLC Number:

DING Cheng-long, LIU Ying, SHEN Yi-xin, GU Hong-ru. QTL Analysis of Traits Relating to Cold Resistance of Zoysia japonica[J]. , 2010, 18(5): 703-707.

丁成龙, 刘颖, 许能祥, 顾洪如. 日本结缕草抗寒相关性状的QTL分析[J]. , 2010, 18(5): 703-707.

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References

[1] 郭海林,刘建秀,朱雪花,等.结缕草属杂交后代抗寒性评价[J].草地学报,2006,14(1):24-28
[2] 黄占兵,金洪.结缕草遗传多样性及AFLP应用研究进展[J].内蒙古农业大学学报(自然科学版),2003,24(3):119-124
[3] Pattona A J,Reicherb Z J.Zoysiagrass species and genotypes differ in their wnter injury and freeze tolerance[J].Crop Science,2007,47:1619-1627
[4] Cai H,Inoue M,Yuyama N,et al.An AFLP-based linkage map of zoysiagrass (Zoysia japonica)[J].Plant Breeding,2004,123(6):543-548
[5] Charles L G.Freezing tolerance of plants:Current understanding and selected emerging concepts[J].Canadian Journal of Botany,2003,81:1216-1223
[6] Philley H W,Watson C E,Krans J V.Inheritance of cold tolerance in St.Augustinegrass[J].Crop Science,1998,38:451-454
[7] 陈儒钢,巩振辉,逯明辉,等.植物抗寒基因工程研究进展[J].西北植物学报,2008,28(6):1274-1280
[8] 贺杰,孙振元,魏建华,等.结缕草生物技术研究进展[J].武汉植物学研究,2006,24(1):74-79
[9] 刘颖,沈益新,顾洪如,等.两结缕草品系对低温胁迫的生理响应研究[J].草地学报,2010,18(2):228-232
[10] 朱根海,刘祖祺,朱培仁.应用Logistic方程确定植物组织低温半致死温度研究[J].南京农业大学学报,1986,(3):11-16
[11] 莫惠栋.Logistic方程及其应用[J].江苏农学院学报,1983,4(2):53-57
[12] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.167-260
[13] Li Manli,Nana Yuyama,Mariko Hirata,et al.Construction of a high-density SSR marker-based linkage map of zoysiagrass (Zoysia japonica Steud.)[J].Euphytica,2009,170:327-338
[14] Van Ooijen J W,Boer M P,Jansen R C,et al.MapQTL 4.0:software for the calculation of QTL positions on genetic maps[M].Plant Research International,Netherlands:Wageningen,2002.77-78
[15] 郑轶琦,刘建秀.草坪草分子遗传图谱的构建与应用研究进展[J].草业学报,2009,18(2):155-162
[16] 郭海林,高雅丹,薛丹丹,等.结缕草属植物抗寒性的遗传分析[J].草业学报,2009,(3):53-58
[17] 潘瑞炽.植物生理学[M].北京:高等教育出版社,2008.285-293
[18] 刘祖琪,张石城.植物抗性生理学[M].北京:中国农业出版社,1994.14:43-44
[19] 张献龙,唐克轩.植物生物技术[M].北京:科学出版社,2004.435-439
[20] Cai Hongwei,Maiko Inoue,Nana Yuyama,et al.Isolation,characterization and mapping of simple sequence repeat markers in zoysiagrass(Zoysia spp.)[J].Theoretical and Applied Genetics,2005,112:158-166
[21] 高世斌.玉米耐旱相关性状的QTL分析[D].雅安:四川农业大学,2004.53-57
[22] Tanksley S D,Nelson J C.Advanced backcross QTL analysis:a method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines[J].Theoretical and Applied Genetics,1996,92:191-203
[23] 潘存红,李爱宏,陈宗祥,等.水稻黑条矮缩病抗性QTL分析[J].作物学报,2009,35(12):2213-2217
[24] 逯晓萍,云锦凤,肖宇红,等.高丹草(高粱×苏丹草)产量及其构成因素的QTL定位与分析[J].华北农学报,2007,22(4):80-85

QTL Analysis of Traits Relating to Cold Resistance of Zoysia japonica

日本结缕草抗寒相关性状的QTL分析

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