Isolation and Identification of High-Quality Lactic Acid Bacteria in Hedysarum fruticosum Pall.Silage

doi:10.11733/j.issn.1007-0435.2012.01.026

›› 2012, Vol. 20 ›› Issue (1): 166-170.DOI: 10.11733/j.issn.1007-0435.2012.01.026

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Isolation and Identification of High-Quality Lactic Acid Bacteria in Hedysarum fruticosum Pall.Silage

SI Bing-wen¹, WANG Zong-li², SUN Qi-zhong², XU Chun-cheng³, LI Feng², TAO Ya²

1. Graduate School, Chinese Academy of Agricultural Science, Beijing 100081, China;
2. Grassland Research Institute, CAAS, Hohhot, Inner Mongolia 010010, China;
3. China Agriculture University, Beijing 100083, China

Received:2011-08-12 Revised:2011-11-08 Online:2012-02-15 Published:2012-07-06

山竹岩黄芪青贮中优质乳酸菌的分离和鉴定

司丙文¹, 王宗礼², 孙启忠², 徐春城³, 李峰², 陶雅²

1. 中国农业科学院研究生院, 北京100081;
2. 中国农业科学院草原研究所, 内蒙古呼和浩特010010;
3. 中国农业大学工学院, 北京100083

作者简介:司丙文(1975- ),男,黑龙江佳木斯人,博士研究生,研究方向为草地资源保护与利用,E-mail:sibingwen@sina.com
基金资助:
现代农业产业技术体系建设专项资金(CARS-35);中央级公益性科研院所基本科研业务费专项资金(中国农业科学院草原研究所);内蒙古自治区自然科学基金项目(2010MS0402)资助

Abstract

Abstract: Three strains of lactic acid bacteria were isolated from Hedysarum fruticosum Pall.silage.The 16S rRNA analysis and traditional identification methods indicated that three strains,Enterococcus mundtii(HF84),Lactococcus lactis subsp.lactis(HF69) and Lactobacillus plantarum(HF49),all were homofermentative lactic acid bacteria.HF84 could not grow at pH 3.0,HF69 could not grow under pH 3.0,5℃ and 6.5% NaCl conditions,while HF49 can grow well at all tested conditions.Through measuring the rate of lactic acid production and the growth curve,HF49 can be selected as excellent strain for preparing lactic acid bacteria additive due to its rapid lactic acid-production and growth speed.

Key words: Hedysarum fruticosum Pall., Silage, Lactic acid bacteria, Isolation, Identification

摘要： 从山竹岩黄芪(Hedysarum fruticosum Pall.)青贮饲料中分离到3株乳酸菌(HF84,HF69,HF49)。经传统鉴定方法及16SrRNA序列分析,菌株HF84为蒙氏肠球菌(Enterococcus mundtii),菌株HF69为乳酸乳球菌乳酸亚种(Lactococcus lactis subsp.Lactis),菌株HF49为植物乳杆菌(Lactobacillus plantarum)。3株乳酸菌均为同型发酵乳酸菌,蒙氏肠球菌在pH 3.0条件下不能生长,乳酸乳球菌乳酸亚种在pH 3.0、温度5℃时及6.5%NaCl溶液中不能生长,植物乳杆菌均可在试验设定条件下生长。通过测定3株乳酸菌的生长曲线和产酸速率,菌株HF49生长速度快、产酸性能好,可作为制备乳酸菌青贮饲料添加剂的优良菌株。

关键词: 山竹岩黄芪, 青贮, 乳酸菌, 分离, 鉴定

CLC Number:

S816.53

SI Bing-wen, WANG Zong-li, SUN Qi-zhong, XU Chun-cheng, LI Feng, TAO Ya. Isolation and Identification of High-Quality Lactic Acid Bacteria in Hedysarum fruticosum Pall.Silage[J]. , 2012, 20(1): 166-170.

司丙文, 王宗礼, 孙启忠, 徐春城, 李峰, 陶雅. 山竹岩黄芪青贮中优质乳酸菌的分离和鉴定[J]. , 2012, 20(1): 166-170.

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Isolation and Identification of High-Quality Lactic Acid Bacteria in Hedysarum fruticosum Pall.Silage

山竹岩黄芪青贮中优质乳酸菌的分离和鉴定

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