The Physiological Response of Desert Grassland Plant Reamuria soongorica under Drought Stress to Exogenous ABA

doi:10.11733/j.issn.1007-0435.2016.05.011

Acta Agrestia Sinica ›› 2016, Vol. 24 ›› Issue (5): 1001-1008.DOI: 10.11733/j.issn.1007-0435.2016.05.011

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The Physiological Response of Desert Grassland Plant Reamuria soongorica under Drought Stress to Exogenous ABA

CHONG Pei-fang¹, ZENG Ji-juan¹, SHAN Li-shan¹, LI Yi¹, YANG Jiang-shan²

1. Forestry College of Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
2. Gansu Provincial Key Lab of Aridland Crops Science, Lanzhou, Gansu Province 730070, China

Received:2016-01-18 Revised:2016-07-05 Online:2016-10-15 Published:2017-01-13

干旱胁迫下荒漠草地植物红砂幼苗对外源ABA的生理响应

种培芳¹, 曾继娟¹, 单立山¹, 李毅¹, 杨江山²

1. 甘肃农业大学林学院, 甘肃兰州 730070;
2. 甘肃省干旱生境作物学重点实验室, 甘肃兰州 730070

作者简介:种培芳(1977-),女,甘肃永登人,博士,教授,研究方向为荒漠植物生理生态,E-mail:zhongpf@gsau.edu.cn
基金资助:
国家自然基金（41461044，31360205，41361100）；教育部博士点基金（20116202120001）；国际科技合作与交流专项项目（2012DFR30830）；甘肃农业大学青年导师基金（GAN-QNDS-01302）资助

Abstract

Abstract:

A pot experiment was conducted to investigate the effect of exogenous abscisic acid (ABA) at the concentrations of 0.1,1,10 and 100 μmol·L^-1 on physiological characters of Reamuria soongorica under different drought stress treatments (CK,light drought, moderate drought and severe drought).And the response in drought resistance of R. soongorica to exogenous ABA was analyzed. The results showed that,under the drought stress the accumulation of osmotic adjustment substances such as proline (Pro),soluble sugar (SS) and soluble protein (SP) in R. soongorica were increased by ABA application. The decrease of superoxide dismutase (SOD), peroxide enzyme (POD) and chlorophyll content (a+b) and the increase of malondiadehyde (MDA) were prevented significantly with sprayed ABA, which could enhance the drought resistance of R. soongorica. The function of ABA on enhancing the drought stress of R. soongorica would be decreased when the concentration of ABA exceeded the limited concentration of 10 μmol·L^-1.The effect of ABA on drought resistance of R. soongorica was not only on some of the physiological characteristics, but on all the measured physiological characteristics. Correlation analysis showed that with the application of certain concentration of ABA,the normal physiological and metabolic functions in leaves of R. soongorica were maintained under drought stress in growth season,which could effectively control the aging process of the leaves.

Key words: Drought stress, Desert grassland, Reamuria soongorica, ABA, Physiological characteristics

摘要：

以2年生红砂（Reamuria soongorica）幼苗为试材，采用盆栽试验研究了正常水分（CK）及轻度、中度和重度干旱胁迫下喷施0.1，1，10和100 μmol·L^-1 4种浓度外源脱落酸（abscisic acid，ABA）对红砂幼苗生理特性的影响，分析了红砂抗旱性对外源ABA的响应。结果表明：干旱胁迫下外源ABA的喷施促进了红砂体内渗透调节物质脯氨酸、可溶性蛋白和可溶性糖的积累，能明显阻止干旱胁迫后红砂体内超氧化物歧化酶（superoxide dismutase，SOD）和过氧化物酶（peroxide enzyme，POD）活性的减弱以及叶绿素含量的降低，抑制受旱红砂叶片丙二醛（malondiadehyde，MDA）增生，从而增强红砂的抗旱性。但是外源ABA对红砂干旱胁迫下的生理调节作用有一定的浓度限制值即10 μmol·L^-1，超过此浓度，作用会减小。外施ABA对红砂抗旱生理机能产生影响并不表现在对某一种生理指标上，而是多个指标的相互关联和相互影响。综合分析表明，干旱胁迫下叶面喷施一定浓度的脱落酸可以维持红砂生长季叶片正常的生理代谢功能，有效控制叶片的衰老进程。

关键词: 干旱胁迫, 荒漠草地, 红砂, 脱落酸, 生理特性

CLC Number:

Q945.78

CHONG Pei-fang, ZENG Ji-juan, SHAN Li-shan, LI Yi, YANG Jiang-shan. The Physiological Response of Desert Grassland Plant Reamuria soongorica under Drought Stress to Exogenous ABA[J]. Acta Agrestia Sinica, 2016, 24(5): 1001-1008.

种培芳, 曾继娟, 单立山, 李毅, 杨江山. 干旱胁迫下荒漠草地植物红砂幼苗对外源ABA的生理响应[J]. 草地学报, 2016, 24(5): 1001-1008.

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References

[1] 赛胜宝. 内蒙古北部荒漠草原带的严重荒漠化及其治理[J]. 干旱区资源与环境,2001,15(4):34-37
[2] 曾彦军,王彦荣,张宝林,等. 红砂种群繁殖特性的研究[J]. 草业学报,2002,11(2):65-70
[3] Editorial Committee of Agricultural and Pastoral Divisions ofAlashan Prefecture(阿拉善盟农牧业区划编辑委员会).1992.Agricultural and Pastoral Divisions of Alashan Prefecture.Huh hot:Inner Mongolia People's Press.30-452(in Chinese)
[4] 宋雪梅,杨九艳,呼格吉乐图. 破碎化生境对红砂meta-种群遗传多样性的影响[J]. 中国草地学报,2014,36(6):29-33
[5] 王彦荣,曾彦军,张宝林,等. 不同退化红砂荒漠草地的水分分配格局[J]. 应用生态学报,2002,5,13(8):962-966
[6] 吴彩霞,周志宇,庄光辉,等. 强旱生植物霸王和红砂地上部营养物质含量及其季节动态[J]. 草业科学,2004,21(3):30-34
[7] 唐庄生,安慧,上官周平. 荒漠草原沙漠化对土壤养分与植被根冠比的影响[J]. 草地学报,2015,23(3):463-468
[8] 杨昊天,李新荣,刘立超,等. 荒漠草地种灌木生物量分配特征[J]. 中国沙漠,2013,33(5):1340-1348
[9] 胡志群,冯学兰,吴楚彬,等. 脱落酸和细胞分裂素对香根草抗旱性的影响[J]. 草地学报,2015,23(6):1220-1225
[10] 雷娅伟,白小明,王婷,等. 脱落酸对3个野生草地早熟禾种质高温胁迫的缓解效应.[J]. 草地学报,2015,23(1):89-100
[11] Xue X X, Hong B S, Yuan Y M, et al. Biotechnological implications from abscisic acid (ABA) roles in cold stress and leaf senescence as an important signal for improving plant sustainable survival under abiotic-stressed conditions[J]. Critical Reviews in Biotechnology,2010,30:222-230
[12] Anderson B E, Ward J M, S chroeder J I. Evidence for an extracelluar reception site for abscisic acid in commelia guardcells[J]. Plant Physiol,1994,104:1177-1183
[13] 于洋,王晶英,肖云. 干旱与外源ABA交互作用对水曲柳苗木光合参数的影响[J]. 东北林业大学学报2009,37(3):41-43,66
[14] 胡玉净,邓丽娟,张杰,等. 干旱胁迫下外源ABA对观赏海棠叶片可溶性蛋白和脱水素积累的影响[J]. 林业科学,2012,48(4):35-42
[15] 汪月霞,索标,赵鹏飞,等. 外源ABA对干旱胁迫下不同品种灌浆期小麦psbA基因表达的影响[J]. 作物学报,2011,37(8):1372-1377
[16] 阮英慧. 外源激素对开花期大豆抗旱生理特性的影响[D]. 哈尔滨:东北农业大学,2012
[17] 郭新红,姜孝成,潘晓玲. 渗透胁迫和外源脱落酸对梭梭幼苗生理特性的影响[J]. 生命科学研究,2000,4(4):337-342
[18] 曹婧,李晓荣,王翠,等. 外源激素ABA影响新疆荒漠盐生植物异子蓬异型种子萌发机制初探[J]. 生态学报,2015,35(20):1-16
[19] 单立山,李毅,石万里,等. 土壤水分胁迫对红砂幼苗生长和渗透调节物质的影响[J]. 水土保持通报,2015,35(6:)106-109.
[20] 邹琦. 植物生理生化实验指导[M]. 北京:中国农业出版社,2003:98-100
[21] 李忠光,李江鸿,杜朝昆,等. 单一提取系统中同时测定五种植物抗氧化酶[J]. 云南师范大学学报(自然科学版),2002,22(6):44-48
[22] 李合生. 植物生理生化实验原理和技术[M]. 北京:北京高等教育出版社,2001,134-170
[23] 萧浪涛,王三根. 植物生理学实验技术[M]. 北京:中国农业出版社,2005,1:65-221
[24] 王忠. 植物生理学[M]. 北京:中国农业出版社,2000:65-66
[25] 邱真静,李毅,种培芳. PEG胁迫对不同地理种源沙拐枣生理特性的影响[J]. 草业学报,2011,20(3):108-114
[26] 郭新红,姜孝成,潘晓玲. 渗透胁迫和外源脱落酸对梭梭幼苗生理特性的影响[J]. 生命科学研究,2000,4(4):337-342
[27] 彭云玲,赵小强,任续伟,等. 干旱胁迫对不同株型玉米大喇叭口期生长的影响[J]. 中国沙漠,2013,33(4):1064-1070
[28] 种培芳,苏世平,李毅. 不同地理种源红砂幼苗对PEG胁迫的生理响应[J]. 草业学报,2013,22(1):185-192
[29] 木合塔尔·扎热. 尖果沙枣幼苗抗旱特性及其对外源脱落酸和外源硅的响应[D]. 乌鲁木齐:新疆农业大学,2009
[30] 张泱. 叶面喷施脱落酸对银中杨几个生理指标的影响[J]. 东北林业大学学报,2005,33(3):97-98
[31] 郝格格,孙忠富,张录强,等. 脱落酸在植物逆境胁迫研究中的进展[J]. 中国农学通报,2009,25(18):212-215
[32] 赵哈林,赵学勇,张铜会. 沙漠化过程中植物的适应对策及植被稳定性机理[M]. 北京:海洋出版社,2004
[33] 李国龙. 作物抗旱生理与分子作用机制研究进展[J]. 中国农学通报,2010,26(23):185-191
[34] 般云刚. 油菜素内脂和甜菜碱诱导番茄幼苗抗旱机理的研究[D]. 重庆:重庆西南大学,2008:24

The Physiological Response of Desert Grassland Plant Reamuria soongorica under Drought Stress to Exogenous ABA

干旱胁迫下荒漠草地植物红砂幼苗对外源ABA的生理响应

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