Evaluation of the Salt Resistance on Agropyron Germplasm at Seedling Stage Based on Chlorophyll Fluorescence Parameters and Physiological Indicator

doi:10.11733/j.issn.1007-0435.2026.02.016

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (2): 543-552.DOI: 10.11733/j.issn.1007-0435.2026.02.016

Evaluation of the Salt Resistance on Agropyron Germplasm at Seedling Stage Based on Chlorophyll Fluorescence Parameters and Physiological Indicator

WEI Ya-qin¹, GUO Mei-ying¹, LIU Ya-ling³, LIU Xi-qiang², ZHAO Yan¹

1. College of Grassland Science, Inner Mongolia Agricultural University, Key Laboratory of Grassland Resources (IMAU), Hohhot, Inner Mongolia 010010, China;
2. Institute of Ecological Protection and Restoration, Chinese Academy of Forestry/Grassland Research Center, National Forestry and Grassland Administration, Beijing 100091, China;
3. Inner Mongolia Pratacultural Technology Innovation Center Co., Ltd, Hohhot, Inner Mongolia 010070, China

Received:2025-03-19 Revised:2025-05-15 Published:2026-01-22

基于叶绿素荧光参数和生理指标对冰草种质资源幼苗期的耐盐性评价

魏雅琴¹, 郭美莹¹, 刘亚玲³, 刘希强², 赵彦¹

1. 内蒙古农业大学草业学院/草地资源教育部重点实验室, 内蒙古呼和浩特 010010;
2. 中国林业科学研究院生态保护与修复研究所/国家林业和草原局草原研究中心, 北京 100091;
3. 内蒙古草业技术创新中心有限公司, 内蒙古呼和浩特 010070

通讯作者: 赵彦，E-mail:zhaoyannmg@imau.edu.cn
作者简介:魏雅琴（1999-），女，满族，内蒙古乌兰察布人，硕士研究生，主要从事草种质资源与育种研究，E-mail:weiyaqininn@163.com；
基金资助:
草种业核心技术攻关项目（SJCZFY2022-1-4）；内蒙古自治区科技计划项目“黄河流域耐盐碱生产生态型优良草品种的高效繁育与加工利用”（2022YFHH0043）；2023年国家草业技术创新中心（筹）重大创新平台建设专项专题（CCPTZX2023W1）；国家自然科学基金项目（32160326）资助

Abstract

Abstract: To identify salt-tolerant germplasm resources of Agropyron cristatum， this study subjected 31 germplasm samples to salt stress treatment with a soil salt content of 0.9%. Chlorophyll fluorescence parameters （phi2， NPQt， qL， Fv/Fm， Fo， Fm， Y（NPQ）， Y（NO）， and Y（II）） and physiological indicators （MDA， Pro， SS， and POD） were determined， and their salt tolerance was comprehensively evaluated using correlation analysis， principal component analysis （PCA）， and cluster analysis. The results showed that the coefficient of variation of the measured indicators of 31 samples ranged from 0.03 to 0.99. PCA indicated that the contribution rates of the top four principal components were 43.152%， 22.847%， 9.746%， and 8.882%， respectively. Cluster analysis suggested that the first group had higher photosynthetic efficiency and POD activity， and lower MDA content； the second group had moderate photosynthetic rate； the photosynthesis and physiological indices of the third group were severely affected. Based on PCA and cluster analysis， combined with changes in physiological responses， six salt-tolerant Agropyron germplasm （20-02， 20-03， 20-06， 20-08， 20-11， 20-17） were screened， most of which belonged to A. latissimum. In conclusion， the salt-tolerant germplasm obtained in this study could be used as the candidate materials for the breeding of new varieties of Agropyron， and also provide germplasm resources for the exploration of excellent salt-tolerant genes.

Key words: Agropyron cristatum, Salt tolerance, ChlorophyII fluorescence parameters, Physiological index

摘要： 为筛选耐盐冰草（Agropyron Gaertn.）种质资源，本研究对野生采集的31份冰草种质资源开展混合中性盐胁迫处理，通过检测叶绿素荧光参数，包括φPSII，NPQt，qL，Fv/Fm，Fo，Fm，Y（NPQ），Y（NO）及Y（II）同时测定MDA，Pro，SS和POD等生理指标，运用相关性分析、主成分分析（PCA）及聚类分析，对种质耐盐性进行综合评价。结果显示，31份冰草材料测定指标变异系数介于0.03~0.99之间。主成分分析显示，前四个主成分贡献率依次为43.152%，22.847%，9.746%和8.882%。聚类分析将种质分为三类：Ⅰ类光合效率与过氧化物酶活性较高，丙二醛含量较低；Ⅱ类光合速率处于中等水平；Ⅲ类光合作用及生理指标显著受盐胁迫影响。基于主成分及聚类分析，并结合生理响应变化，筛选出6份耐盐冰草种质资源（20-02，20-03，20-06，20-08，20-11，20-17），其中多数为扁穗冰草。本研究获得的冰草耐盐种质资源可作为冰草新品种选育候选材料，也为挖掘优良耐盐基因提供种质基础。

关键词: 冰草, 耐盐性, 叶绿素荧光参数, 生理指标

WEI Ya-qin, GUO Mei-ying, LIU Ya-ling, LIU Xi-qiang, ZHAO Yan. Evaluation of the Salt Resistance on Agropyron Germplasm at Seedling Stage Based on Chlorophyll Fluorescence Parameters and Physiological Indicator[J]. Acta Agrestia Sinica, 2026, 34(2): 543-552.

魏雅琴, 郭美莹, 刘亚玲, 刘希强, 赵彦. 基于叶绿素荧光参数和生理指标对冰草种质资源幼苗期的耐盐性评价[J]. 草地学报, 2026, 34(2): 543-552.

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Evaluation of the Salt Resistance on Agropyron Germplasm at Seedling Stage Based on Chlorophyll Fluorescence Parameters and Physiological Indicator

基于叶绿素荧光参数和生理指标对冰草种质资源幼苗期的耐盐性评价

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