Abstract: In this study, some enzyme activities of tall fescue seed of the harvested year during germination under different accelerated ageing periods are discussed. By analysing the activities of phosphoglucose isomerase(PGI), malic dehydrogenase(MDH)and combined glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase(G-6-PDH+6-PGDH), we found that, the activities of phosphoglucose isomerase in tall fesce seed decreased and malic dehydrogenase incredased during germination in each accelerated ageing period treatment. At the present time of combined activity peak of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase was peostponed and decreased during germination with the inreasing of acceleraled ageing period treatment.The activities of phosphoglucose isomerase, malic dehydrogenase , and combined glucose 6-phosophate dehydrogenase and 6-phosphogluconate dehydrogenase decreased remarkably with the increasing of accelelerated ageing period.This reflected the fact that the activities of respiratory pathway:glycolysiis(EMP), kreds cycle(TCA)and pentose phosphate(PPP)droppe in acceleraed ageing seed, and this was the one reason for decreasing of germination speed and germination rate of accelerated ageing seed.

Key words: Tall fescue seed, Enzyme activities, Accelerated ageing

摘要： 对当年收获的高羊茅种子进行人工老化后发芽过程的磷酸己糖异构酶活性、葡萄糖-6-磷酸脱氨酶和6-磷酸葡萄糖酸脱氢酶联合活性以及苹果酸脱氨酶活性的测定结果表明,在老化处理的高羊茅种子中,磷酸已糖异构酶活性随着发芽时间的延长呈下降趋势,而苹果酸脱氢酶活性则呈上升趋势。随着老化程度的增加,葡萄糖-6-磷酸脱氢酶和6-磷酸葡萄糖酸脱氢酶联合活性的峰值在发芽过程中出现的时间推迟,且峰值降低。随着人工加速老化强度的增加,磷酸己糖异构酶活性、苹果酸脱氢酶活性、葡萄糖-6-磷酸脱氢酶和6-磷酸葡萄糖酸脱氨酶联合活性均表现为大幅度地降低。老化可使种子前发过程中的糖酵解、三羧酸循环和磷酸戊糖等代谢途径受到限制,影响种子萌发过程中物质的分解与合成,进而造成种子发芽推迟、发芽率降低。

关键词: 高羊茅种子, 酶活性, 加速老化