Abstract: Based on saving cost and time, and increasing the turnover rate in the determination of n alkane concentrations so as to facilitate the application of the n alkane technique in large scale studies of herbage intake were the goal of the study on the modifications of the chemical analytical procedure to be described in this paper.The simplification,eliminating silica gel and injecting the raw extracts into the gas chromatography column,were evaluated of three pasture species:Miscanthus sinensis,Zoysia japonica and Lolium perenne in 2000.The simplified procedure of the n alkane determination was compared with other two ordinary procedures.The results showed that a simplified procedure by eliminating silica gel and injecting the raw extracts into the gas chromatography column could be used for the n alkane determination of Miscanthus sinensis and Lolium perenne.However, the nalkane concentrations of Zoysia japonica without purification by silica gel were not able to get correctly, for the peak of contaminated substances interfered to the peak of C₃₄.Though the samples used in this experiment were supposed to be insufficient to the final conclusion,it is indicated that the procedure simplification should be used cautiously in the different herbage analysis,too.

Key words: analytical procedure, Lolium perenne, Miscanthus sinensis, nalkane, Zoysia japonica.

摘要： 植物细胞壁中链烷含量测定分析研究的目的是不降低测定分析精度的同时,节约时间和费用以便将来用于大量样品的分析。本研究首次把链烷分析提纯过程中省略用作纯化的硅胶,直接将含有链烷但未经硅胶提纯的原提取液注入气相色谱仪中测定,将世界通用的两种链烷分析法作为对照。结果表明,硅胶能否省略应根据不同牧草而定。本实验中所用的三种牧草,以省略硅胶而简化的链烷分析可用于多年生黑麦草和芒,而不能用于日本结缕草中链烷的测定。

关键词: 链烷, 分析, 多年生黑麦草, 芒, 日本结缕草