Acta Agrestia Sinica

›› 2005, Vol. 13 ›› Issue (3): 190-193.DOI: 10.11733/j.issn.1007-0435.2005.03.004

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RAPD Analysis of the Genetic Diversity of 16 Natural Wheatgrass Populations(Agropyron Cristatum)

LI Jing-xin1,2, YUN Jin-feng2, GUO Jun1   

  1. 1. Agronomy College, Inner Mongolia Nationalities University, Tongliao, Inner Mongolia Autonomous Region 028042, China;
    2. Ecology and Environmental Science College, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia Autonomous Region 010019, China
  • Received:2004-05-08 Revised:2004-12-25 Online:2005-08-15 Published:2005-08-15

16个天然冰草种群遗传多样性RAPD分析

李景欣1,2, 云锦凤2, 郭军1   

  1. 1. 内蒙古民族大学, 通辽, 028042;
    2. 内蒙古农业大学, 呼和浩特, 010019
  • 作者简介:李景欣(1968- ),女,蒙古族,内蒙古通辽市人,副教授,博士,主要从事植物遗传育种的教学与科研,已发表学术论文10余篇
  • 基金资助:
    “863计划”资助项目,项目编号2002AA241101

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Abstract: Eleven random primers (10bp)were selected from 57 primers for RAPD analysis of 352 wheatgrass individuals which were collected from 16 natural populations of Inner Mongolia. A hundred and three polymorphic loci were detected from 125 loci. Calculated by Shannon Index, the average genetic diversity was 2.19, the percentage of genetic variation were 60% within each population and 40% among the populations. The interpopulation similarity range was 0.7702～0.9776 and the interpopulation genetic distance range was (0.0226～0.2611). Through cluster analysis, the 16 populations were divided into 4 categories. The populations with similar ecological environment and prototype clustered together, a fact basically consistent with the result of morphological research, indicating that species variation is closely related to the environment.

Key words: Agropyron cristatum, Genetic diversity, Genetic similarity, Genetic distance, Cluster analysis, RAPD(Random Amplified Polymorphic DNA), Population

摘要： 采用57个10 bp随机引物,对16个天然冰草(Agropyron cristatum)种群352个单株的基因组DNA进行RAPD多态性检测。结果表明:从57个RAPD引物中选出多态性标记的引物11个;检测出125个位点,102个显多态性;用Shan-non多样性指数量化的遗传多态度为2.19,种群内和种群间遗传变异比例分别为60%和40%,种群间的遗传相似度在0.7702~0.9776之间,遗传距离的变化范围为0.0226~0.2611;聚类分析结果表明:16个种群大致可分为4类,生境和表型相近的种群基本聚为一类,和形态学研究的结果基本一致,证明物种变异与生境密切相关。

关键词: 冰草, 遗传多样性, 遗传相似度, 遗传距离, 聚类分析, 随即扩增多态DNA, 种群

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