Acta Agrestia Sinica

›› 2009, Vol. 17 ›› Issue (2): 193-196.DOI: 10.11733/j.issn.1007-0435.2009.02.011

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Study on Plant Regeneration from Midnight Ⅱ

MA Hui-ling, ZHAO Xiao-qiang, ZHOU Wan-hai, WU Xiang   

  1. 1. Pratacultural College, Gansu Agricultural University, Lanzhou, Gansu Province, 730070, China;
    2. Key Laboratory of Grassland Ecosystem, Ministry of Education, Lanzhou, Gansu Province, 730070, China;
    3. Sino-U.S.Center for Grazingland Ecosystem Sustainability, Lanzhou, Gansu Province, 730070, China
  • Received:2008-04-09 Revised:2008-10-10 Online:2009-04-15 Published:2009-04-15

草地早熟禾午夜2号植株再生研究

马晖玲, 赵小强, 周万海, 吴翔   

  1. 1. 甘肃农业大学草业学院, 甘肃, 兰州, 730070;
    2. 草业生态系统教育部重点实验室, 甘肃, 兰州, 730070;
    3. 中-美草地畜牧业可持续研究中心, 甘肃, 兰州, 730070
  • 作者简介:马晖玲(1966- ), 女, 回族, 甘肃兰州人, 教授, 博士, 主要从事草坪草、牧草育种研究, E-mail:mahl@gsau.edu.cn
  • 基金资助:
    甘肃农业大学草业学院草业科学国家级重点学科学术骨干科研项目暨草业生态系统教育部省部共建重点实验室资助项目(CY-GG-2006-10);甘肃省教育厅项目(0702-03)

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Abstract: In order to establish the basis for protoplast culture and cell fusion of Poa pratensis L.,the plant regeneration was studied using the mature seeds of Midnight Ⅱ as explants.Results indicate that the optimal callus inducement medium was MS+1 mg/L 2,4-D+0.1 mg/L 6-BA+3% sucrose+0.7% agar and the callus inducement rate was 52.3%;the best medium of subculture was MS+1 mg/L 2,4-D+0.3 mg/L 6-BA+3% sucrose+0.7% agar;the best medium of differentiation was MS+0.5 mg/L NAA + 1 mg/L 6-BA+3% sucrose+0.6% agar and the callus differentiation rate was 57.5%.The rooting medium was the same as the medium of differentiation.100 d was needed to obtain the regenerated plantlet from donor materials.

Key words: Poa pratensis L., Callus, Plant regeneration

摘要： 以草地早熟禾(Poa pratensis L.)品种午夜2号成熟种子为外植体,进行植株再生研究,建立高频再生体系,为草地早熟禾进行原生质体培养和细胞融合奠定基础。结果表明:诱导愈伤组织的最佳培养基为MS+2,4-D(1mg/L)+6-BA(0.1 mg/L)+3%蔗糖+0.7%琼脂,其诱导率为52.3%;最佳继代培养基为MS+2,4-D(1mg/L)+6-BA(0.3mg/L)+3%蔗糖+0.7%琼脂;最佳分化培养基为MS+NAA(0.5 mg/L)+6-BA(1 mg/L)+3%蔗糖+0.6%琼脂、分化率为57.5%;生根培养基同分化培养基,供体材料经100d的培养后获得再生植株。

关键词: 草地早熟禾, 愈伤组织, 植株再生

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