Abstract: The physiological mechanisms of cold-resistance and heat-tolerance in Zoysia matrella,as well as its maintenance management,have been reported,however little information is available for its resistance pertaining to molecular mechanisms.The differential expression proteins of leaves in the turfgrass under low temperature and high temperature treatments were studied by the method of differential proteomics,to search for molecular mechanisms of Z.matrella which was relatively tolerant to high temperature while sensitive to chilling.There were 35 protein spots showing different expression patterns in the turfgrass after treated with different temperature stress.Twenty six proteins were successfully identified.Further analysis showed that some of the expressed proteins were up-regulated under the low temperature treatment,such as functioning to increase signal transduction and activate oxygen scavenging and protein defense,would enhance a resistance ability in Z.matrella to decrease low temperature stress.Also,some proteins were found to be down-regulated in Z.matrella under the low temperature treatment.Those down-regulated proteins resulted in an initial increase,and then followed by a decrease of respiration rate.In turn,photosythesis declined,proteins degraded,and transportation ability reduced,consequently led to decreased resistance potential in Z.matrella under the low temperature treatment.These up-regulated proteins strengthened signal discernableness and its transduction,increased photosythesis and respiration,promoted secondary metabolism,and activated oxygen scavenging.They enhanced protein defense and synthesis,in turn,led to a stronger resistance capability in Z.matrella under the high temperature treatment.

Key words: Zoysia matrella, Temperature stress, Proteomics

摘要： 为阐明沟叶结缕草(Zoysia matrella (L.) Merr.)相对耐高温而不耐低温的分子机理,于2008年5月应用差异蛋白质组学方法分析了沟叶结缕草在低温(5℃)和高温(40℃)胁迫下的叶片蛋白质组差异表达,共检测到35个差异蛋白质点,其中26个得到鉴定。分析表明:沟叶结缕草在低温胁迫下,一方面加强了胞内信号转导、活性氧的清除、蛋白质防御以提高其低温适应性;另一方面,由于一些蛋白质的下调表达,使得其低温下植物体呼吸作用表现为先增强后减弱、光合作用下降、蛋白降解、细胞的转运能力减弱,因而抗低温性能下降。在高温胁迫下,则通过一些蛋白质的上调表达,加强了体内信号识别和转导、光合作用、呼吸作用、次生代谢作用、活性氧的清除、蛋白质防御及合成等功能,沟叶结缕草表现出相对较强的高温抗逆性。

关键词: 沟叶结缕草, 温度胁迫, 蛋白质组学