Abstract: Using cotyledons and hypocotyls of wild Medicago falcata L. from Hulunbeier grassland as explants, tissue culture and regeneration system of Medicago falcata L. were studied for further transgenic experiments providing a good receptor. Results showed that the optimal medium for callus induction was MS+1.5 mg·L^-1 2,4-D+0.4 mg·L^-1 6-BA+3% sucrose+0.7% agar, the highest callus induction ratio was 100%. The optimal medium for embryogenic callus was MS+0.5 mg·L^-1 KT+0.1 mg·L^-1 NAA+2% sucrose+0.7% agar, inducted ratio of embryogenic callus was 81.5%.The optimal medium for differentiated buds was MS+0.25 mg·L^-1 KT+0.05 mg·L^-1 NAA+2% sucrose+0.7% agar, differentiated ratio of buds was 36.5%. The optimal rooting medium was 1/2MS+0.5 mg·L^-1 NAA+1.5% sucrose+0.7% agar, rooting ratio was 93.3%. Callus induction results showed that the tissue culture of Medicago falcata L. had different induction ratio between individuals with the same hormone.

Key words: Medicago falcata L., Tissue culture, Regeneration system, Callus

摘要： 以呼伦贝尔草原野生黄花苜蓿(Medicago falcata L.)无菌苗的子叶和下胚轴为外植体,对其组织培养及再生体系进行系统的研究,以期为其下一步转基因试验提供良好的受体。结果表明:最佳愈伤诱导培养基为MS+1.5 mg·L^-1 2,4-D+0.4 mg·L^-1 6-BA+3%蔗糖+0.7%琼脂,下胚轴和子叶的最佳愈伤诱导率均可达到100%。最佳胚性愈伤形成培养基为MS+0.5 mg·L^-1 KT+0.1 mg·L^-1 NAA+2%蔗糖+0.7%琼脂,胚性愈伤形成率为81.5%;最佳分化芽形成培养基为MS+0.25 mg·L^-1 KT+0.05 mg·L^-1 NAA+2%蔗糖+0.7%琼脂,分化芽形成率为36.5%。最佳生根培养基为1/2MS+0.5 mg·L^-1 NAA+1.5%蔗糖+0.7%琼脂,生根率为93.3%。愈伤诱导结果显示,供试黄花苜蓿材料个体间组织培养再生性存在较大差异,在同一激素水平上有大约1/3植株的愈伤状态优于其他植株。

关键词: 黄花苜蓿, 组织培养, 再生体系, 愈伤