Advances on Soil Microbiological Characteristics of Grassland Ecosystems and Its Response to Human Disturbances

doi:10.11733/j.issn.1007-0435.2014.06.003

Acta Agrestia Sinica ›› 2014, Vol. 22 ›› Issue (6): 1163-1170.DOI: 10.11733/j.issn.1007-0435.2014.06.003

Previous Articles Next Articles

Advances on Soil Microbiological Characteristics of Grassland Ecosystems and Its Response to Human Disturbances

TAN Hong-yan^1,2, CHEN Bao-rui^1,2, RAN Rui-rui^1,2, XIN Xiao-ping^1,2, TAO Jin³

1. Hulunber Grassland Ecosystem Research Station, Beijing 100081, China;
2. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
3. Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010019, China

Received:2014-01-23 Revised:2014-04-01 Online:2014-12-15 Published:2014-12-01

草地土壤微生物特性及其对人为干扰响应的研究进展

谭红妍^1,2, 陈宝瑞^1,2, 闫瑞瑞^1,2, 辛晓平^1,2, 陶金³

1. 呼伦贝尔草原生态系统国家野外科学观测研究站, 北京 100081;
2. 中国农业科学院资源区划所草地科学研究室, 北京 100081;
3. 内蒙古农业大学, 内蒙古呼和浩特 010019

通讯作者: 辛晓平
作者简介:谭红妍(1991-),女,山东聊城人,硕士研究生,主要从事草地土壤微生物生态研究,E-mail:hongyantan@yeah.net
基金资助:
公益性行业(农业)科研专项 (201303060);国际科技合作项目(2012DFA31290);现代农业产业技术体系建设专项(CAES-35);国家自然科学基金项目(41101216)资助

Abstract

Abstract:

Soil microorganism is an important part of grassland ecosystem and plays a significant role in the degradation of organic matter, nutrient cycling and plant diversity maintenance, etc. Taking advantage of the environmental sensitivity of soil microorganism, the degenerate system of grassland is able to be predicted early. The characteristics of soil microorganisms in natural grassland, and the advances of property changes of soil microorganism under different human disturbances including grazing, mowing, enclosure and nitrogen fertilizer addition treatments are reviewed in this paper. The prospects of research directions are forecast at the same time.

Key words: Grassland ecosystem, Soil microorganism, Microbial biomass, Microbial community structure, Human disturbance

摘要：

土壤微生物是草地生态系统的重要组成部分,在养分循环、有机质降解和植物多样性维持等方面发挥着重要作用.利用土壤微生物对环境变化的敏感性,以其特性变化来表征草地生态系统当前的状况,能较早的对退化系统做出预警.文章综述了天然草地土壤微生物特性,以及在放牧、刈割、围封、氮肥添加4种不同人为干扰下土壤微生物特性变化的研究进展,并对未来研究进行了展望.

关键词: 草地生态系统, 土壤微生物, 微生物量, 微生物群落结构, 人为干扰

CLC Number:

TAN Hong-yan, CHEN Bao-rui, RAN Rui-rui, XIN Xiao-ping, TAO Jin. Advances on Soil Microbiological Characteristics of Grassland Ecosystems and Its Response to Human Disturbances[J]. Acta Agrestia Sinica, 2014, 22(6): 1163-1170.

谭红妍, 陈宝瑞, 闫瑞瑞, 辛晓平, 陶金. 草地土壤微生物特性及其对人为干扰响应的研究进展[J]. 草地学报, 2014, 22(6): 1163-1170.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2014.06.003

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2014/V22/I6/1163

References

[1] 李博,雍世鹏,李瑶,等. 中国的草原[M]. 北京:科技出版社,1990:80
[2] 陈佐忠,汪诗平. 中国典型草原生态系统[M]. 北京:科技出版社,2000:10
[3] 徐柱. 面向21世纪的中国草地资源[J]. 中国草地,1998(5):1-8
[4] 张新时. 草地的生态经济功能及其范式[J]. 科技导报,2000(8):3-5
[5] 张瑞娟,李华,林勤保,等. 土壤微生物群落表征中磷脂脂肪酸(PLFA)方法研究进展[J]. 山西农业科学,2011,39(9):1020-1024
[6] Griffiths B S, Ritz K, Bardgett R D, et al. Ecosystem response of pasture soil communities to fumigation-induced microbial diversity reductions: An examination of the biodiversity-ecosystem function relationship [J]. Oikos-A Journal of Ecology,2000,90(2):279-294
[7] Bissett A, Burke C. Bacterial community shifts in organically perturbed sediments [J]. Environmental Microbiology,2007,9(1):46-60
[8] 贺金生,王政权,方精云. 全球变化下的地下生态学:问题与展望[J]. 科学通报,2004,49(13):1226-1233
[9] Franzlubbers A J, Hons F M, Zuber D A. Seasonal changes in soil microbial biomass and mineralizable C and N in Wheat management systems [J]. Soil Biology & Biochemistry,1994,26(11):1469-1475
[10] 刘世贵,葛绍荣. 川西北退化草地土壤微生物数量与区系研[J]. 草业学报,1994,3(4):70-76
[11] Holt J A. Grazing pressure and soil carbon,microbial biomass and enzyme activities in semi-arid northeastern Australia [J]. Applied Soil Ecology,1997,5(2):143-149
[12] Dalal R C, Henderson P A, Glasby J M. Organic matter and microbial biomass in a vertisol after 20 yr of zero-tillage [J]. Soil Biology & Biochemistry,1991,23(5):435-441
[13] 曹淑宝,王立群. 放牧对草原土壤微生物影响研究进展[J]. 中国农学通报,2011,27(29):271-275
[14] 钟文辉,蔡祖聪. 土壤微生物多样性研究方法[J]. 应用生态学报,2005,15(5):899-904
[15] 吴永胜,李浩,马万里,等. 分子生物学技术在草原土壤微生物研究中的应用[J]. 内蒙古师范大学学报:自然科学汉文版,2008,37(2):214-221
[16] Jim H. Soil Microbial Communities restoration Ecology: Facilitators or Followers [J]. Science,2009,325(5940):573-574
[17] Amann R I, Ludwing. Phylogenetic identification and in situdetection of individual microbial cells without cultivation [J]. Microbiology and Molecular Biology Reviews,1995,59(1):143-169
[18] 刘国华,叶正芳,吴为中. 土壤微生物群落多样性解析法:从培养到非培养[J]. 生态学报,2012,32(14):4421-4433
[19] 张秋芳,刘波,林营志,等. 土壤微生物群落磷脂脂肪酸PLFA生物标记多样性[J]. 生态学报,2009,29(8):4127-4137
[20] 吴愉萍. 基于磷脂脂肪酸(PLFA)技术分析土壤微生物群落结构多样性的研究[D]. 杭州:浙江大学,2009:2-10
[21] 颜慧,蔡祖聪,钟文辉. 磷脂脂肪酸分析方法及其在土壤微生物多样性研究中的应用[J]. 土壤学报,2006,43(5):851-85
[22] Balkwill D L, Leach F R, Wilson J T, et al. Equivalenee of mierobial biomass measures based on membrane lipid and cell wall components,adenosine triphosphate and direct counts in subsurface aquifer sediments [J]. Microbial Ecology,1988,16(1):73-84
[23] 郑华,欧阳志云,方治国,等. BIOLOG在土壤微生物群落功能多样性研究中的应用[J]. 土壤学报,2004,41(3):456-461
[24] 车玉伶,王慧,胡洪营,等. 微生物群落结构和多样性解析技术研究进展[J]. 生态环境,2005,14(1):127-133
[25] Ibekwe A M, Kennedy A C. Phospholipid fatty acid profiles and carbon utilization patterns for analysis of microbial community structure under field and greenhouse conditions [J]. FEMS Microbiology Ecology,1998,26(1):151-163
[26] 李丹,王秋玉. 变性梯度凝胶电泳及其在土壤微生物生态学中的应用[J]. 中国农学通报,2011,27(3):6-9
[27] Muyzer G, Waal E C, Uitterlinden A G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA [J]. Applied and Environmental Microbiology,1993,59(3):695-700
[28] Caldern F J, Jackson L E, Scow K M, et al. Microbial responses to simulated tillage and in cultivated and uncultivat ed soils [J]. Soil Biology & Biochemistry,2000,32(11):1547-1559
[29] 甄莉娜,王康,杨俊霞,等. 农牧交错带不同利用方式下土壤菌群数量及剖面分布特征[J]. 中国草地学报,2012,34(2):87-91
[30] 韩芳,邵玉琴,赵吉,等. 皇甫川流域不同土地利用方式下的土壤微生物多样性[J]. 内蒙古大学学报:自然科学版,2003,34(3):289-303
[31] 文倩,林启美,赵小蓉,等. 北方农牧交错带林地、耕地和草地土壤微生物群落结构特征的PLFA分析[J]. 土壤学报,2008,45(2):321-327
[32] 王国荣,陈秀蓉,张俊忠,等. 东祁连山高寒灌丛草地土壤微生物生理功能群的动态分布研究[J]. 草业学报,2011,20(2):31-38
[33] 文都日乐,李刚,杨殿林,等. 呼伦贝尔草原土壤固氮微生物nifH 基因多样性与群落结构[J]. 生态学杂志,2011,30(4):790-797
[34] 李刚,范宝莉,文都日乐,等. 呼伦贝尔针茅草原土壤真菌群落结构分析[J]. 土壤学报,2011,48(5):1096-1101
[35] 张俊忠,陈秀蓉,杨成德,等. 东祁连山高寒草地土壤可培养真菌多样性分析[J]. 草业学报,2010,16(2):124-132
[36] Poly F, Ranjard L, Nazaret S, et al. Comparison of nifH gene pools in soils and soil microenvironments with contrasting properties[J]. Applied and Environmental Microbiology, 2001,67(5):2255-2262
[37] Marschner P, Yang C H, Lieberei R, et al. Soil and plant specific effects on bacterial community composition in the rhizosphere [J]. Soil Biology & Biochemistry,2001,33(11):1437-1445
[38] 蔡晓布,周进,钱成,等. 不同退化程度高寒草原土壤微生物活性变化特征研究[J]. 土壤学报,2008,45(6):1110-1118
[39] 高雪峰,武春燕,韩国栋. 不同利用强度下草原土壤微生物的生物量和数量的动态研究[J]. 干旱区资源与环境,2011,25(5):188-191
[40] 吴永胜,马万里,李浩,等. 内蒙古退化荒漠草原土壤有机碳和微生物生物量碳含量的季节变化[J]. 应用生态学报,21(2):321-316
[41] Bardgett R D, Lovell R D, Hobbs P J, etal. Seasonal changes in soil microbial communities along a fertility gradient of temperate grasslands [J]. Soil Biology and Biochemistry,1999,31(7):1021-1030
[42] 李梓正,朱立博,林叶春,等. 呼伦贝尔草原不同退化梯度土壤细菌多样性季节变化[J]. 生态学报,2010,30(9):2883-2889
[43] 邵玉琴,敖晓兰,宋国宝,等. 皇甫川流域退化草地和恢复草地土壤微生物生物量的研究[J]. 生态学杂志,2005,24(5):578-580
[44] 赵吉,廖仰南,张桂枝,等. 草地生态系统的土壤微生物生态[J]. 中国草地,1999(3):57-67
[45] 姚拓,龙瑞军. 天祝高寒草地不同扰动生境土壤三大类微生物数量动态研究[J]. 草业学报,2006,15(2):93-99
[46] 蔡晓布,钱成,张永清. 退化高寒草原土壤生物学性质的变化[J]. 应用生态学报,2007,18(8):1733-1738
[47] 尚占环,丁玲玲,龙瑞军,等. 江河源区退化高寒草地土壤微生物与地上植被及土壤环境的关系[J]. 草业学报,2007,16(1):34-40
[48] 吴永胜,马万里,李浩,等. 内蒙古退化荒漠草原土壤细菌群落结构特征[J]. 生态学报,2010,30(23):6355-6362
[49] 林超峰,陈占全,薛泉宏,等. 青海三江源区植被退化对土壤养分和微生物区系的影响[J]. 应用与环境生物学报,2007,13(6):788-793
[50] 何亚婷,董云社,齐玉春,等. 草地生态系统土壤微生物量及其影响因子研究进展[J]. 地理科学进展,2010,29(11):1350-1359
[51] 张成霞,南志标. 不同放牧强度下陇东天然草地土壤微生物三大类群的动态特征[J]. 草业科学,2010,27(11):131-136
[52] 闫瑞瑞,闫玉春,辛晓平,等. 不同放牧梯度下草甸草原土壤微生物和酶活性研究[J]. 生态环境学报,2011,20(2):259-265
[53] 高雪峰,武春燕,韩国栋,等. 放牧对典型草原土壤中几种生态因子影响的研究[J]. 干旱区资源与环境,2010,24(4):130-133
[54] 邵玉琴,杨桂霞,崔宇新,等. 锡林郭勒典型草原不同放牧强度下土壤微生物数量的分布特征[J]. 中国草地学报,2011,33(2):63-68
[55] 宋俊峰,韩国栋,张功,等. 放牧强度对草甸草原土壤微生物数量和微生物生物量的影响[J]. 内蒙古师范大学学报,2008,37(2):237-239
[56] Bardgett R D, Hobbs P J, Frostegård Å. Changes in soilfungal: bacterial biomass ratios following reductions in the intensity of management of an upland grassland [J]. Biology and Fertility of Soils,1996,22(3):261-264
[57] Wang K H, McSorleya R, Bohlenb P, et al. Cattle grazingincreases microbial biomass and alters soil nematode communities in subtropical pastures [J]. Soil Biology & Biochemistry,2006,38(7):1956-1965
[58] Kieft T L. Grazing and plant-canopy effects on semiarid soil microbial biomass and respiration[J]. Biology Fertility of Soils,1994,18(2):155-162
[59] Moussa A S, Rensburg L A, Kellner K, et al. Soil microbial biomass in semi-arid communal sandy rangelands in the Western Bophrima district, South Africa [J]. Applied Ecology and Environmental Research,2007,5(1): 43-56
[60] 赵帅,张静妮,赖欣,等. 放牧与围栏内蒙古针茅草原土壤微生物生物量碳、氮变化及微生物群落结构PLFA分析[J]. 农业环境科学学报,2011,30(6):1126-1134
[61] Clegg C D. Impact of cattle grazing and inorganic fertiliser additions to managed grasslands on the microbial community composition of soils [J]. Applied Soil Ecology,2006,31(1):73-82
[62] Ingram L J, Stahl P D, Schuman G E, et al. Grazing Impacts on Soil Carbon and Microbial Communities in a Mixed-Grass Ecosystem [J]. Soil Biology & Biochemistry,2008,72(4):939-948
[63] 高英志,韩兴国,汪诗平. 放牧对草原土壤的影响[J]. 生态学报,2004,24(4):790-797
[64] 朱瑞芬,唐凤兰,张月学,等. 不同利用方式对东北羊草草地土壤微生物数量的影响[J]. 草地学报,2012,20(5):842-847
[65] 邵玉琴,赵吉,刘钟龄,等. 割草频率对土壤微生物数量和植物地下生物量的影响[J]. 生态学杂志,2006,25(10):1191-1195
[66] 邹雨坤,张静妮,杨殿林,等. 不同利用方式下羊草草原土壤生态系统微生物群落结构的PLFA分析[J].草业学报,2011,20(4):27-33
[67] Hilde A, Pål A O. Relative importance of burning, mowing and species translocation in the restoration of a former boreal hayfield: Responses of plant diversity and the icrobial community [J]. Journal of Applied Ecology,2005,42(2):337-347
[68] Denef K, Roobroeck D, Mihiri C W, etal. Microbial community composition and rhizodeposit-carbon assimilation in differently managed temperate grassland soils [J]. Soil Biology & Biochemistry,2009,41(1):144-153
[69] 包青海,宝音陶格涛,仲延凯,等. 不同轮割制度对典型草原主要种群的影响[J]. 应用生态学报,2005,16(12):2333-2338
[70] 邵玉琴,刘钟龄,贾志斌,等. 不同治理措施对退化草原土壤可培养微生物区系的影响[J]. 中国草地学报,2011,33(5):77-81
[71] 张海芳,李刚,宋晓龙,等. 内蒙古贝加尔针茅草原不同利用方式土壤微生物功能多样性[J]. 生态学杂志,2012,31(5):1143-1149
[72] 沈艳,马红彬,谢应忠,等. 宁夏典型草原土壤微生物特征对不同管理方式的响应[J]. 草业科学,2012,29(6):863-868
[73] 单贵莲,初晓辉,罗富成,等. 围封年限对典型草原土壤微生物及酶活性的影响[J]. 草原与草坪,2012,32(1):1-6
[74] Plassart P, Vinceslas M K, Gangneux C, et al. Molecular and functional responses of soil microbial communities under grassland restoration [J]. Agriculture,Ecosystems and Environment,2008,127(3):286-293
[75] Mckinley V L, Peacock A D, White D C. Microbial community PLFA and PHB responses to ecosystem restoration in tallgrass prairie soils [J]. Soil Biology & Biochemistry,2005,37(10):1946-1958
[76] Lovell R D, Jarvis S C, Bardgett R D. Soil microbial biomass and activity in long-term grassland: Effects of management changes [J]. Soil Biology & Biochemistry,1995,27(7):969-975
[77] Lovell R D, Hatch D J. Stimulation of microbial activity following spring application of nitrogen [J]. Soil Biology & Biochemistry,1998,26(1):28-30
[78] Bradley K, Drijber R A, Knops J. Increased N availability in grassland soils modifies their microbial communities and decreases the abundance of arbuscular mycorrhizal fungi [J]. Soil Biology & Biochemistry,2006,38(1):1583-1595
[79] Dijkstra F A, Hobbie S E, Reich P B, et al. Divergent effects of elevated CO₂, N fertilization, and plant diversity on soil C and N dynamics in a grassland field experiment [J]. Plant and Soil,2005,272(1/2):41-52
[80] Johnson D, Leake J R, Read D J. Liming and nitrogen fertilization affects phosphatase activities, microbial biomass and mycorrhizal colonization in upland grassland[J]. Plant and Soil,2005,271(1-2):157-164
[81] Liu W X, Xu W H, Han Y , et al. Responses of microbialbiomass and respiration of soil to topography, burning and nitrogen fertilization in a temperate steppe [J]. Biology Fertility of Soils,2007,44(2):259-268
[82] 何亚婷,齐玉春,董云社,等. 外源氮输入对草地土壤微生物特性影响的研究进展[J]. 地球科学进展,2010,25(8):965-973
[83] 毕捷,张乃莉,梁宇,等. 氮水添加对半干旱地区草原土壤微生物群落结构的交互影响[J]. 广东农业科学,2012(19):166-170
[84] 毕捷,张乃莉,梁宇,等. 施氮和增雨对内蒙古半干旱地区草原土壤微生物群落碳源利用潜力的影响[J]. 中国生态农业报,2012,20(12):1586-1593
[85] 张惠文,张倩茹,周启星,等. 分子微生物生态学及其研究进展[J]. 应用生态学报,2003,12(2):286-292
[86] 吕昌勇,陈朝银,葛锋,等. 微生物分子生态学研究方法的新进[J]. 中国生物工程杂志,2012,32(8):111-118

Advances on Soil Microbiological Characteristics of Grassland Ecosystems and Its Response to Human Disturbances

草地土壤微生物特性及其对人为干扰响应的研究进展

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	JIN Yan-xia, CHEN Zhe, ZHOU Hua-kun, FU Jing-jing. Research Progress of Canopy Interception of Vegetation in Grassland Ecosystem [J]. Acta Agrestia Sinica, 2021, 29(S1): 1-9.
[2]	LIN Chun-ying, LI Xi-lai, SUN Hai-song, SUN Hua-fang, MA Cheng-biao, HAN Hui-bang, ZHANG Yu-Peng, LI Cheng-yi. Responses of Soil Organic Carbon Component on Different Degrees of Degradation of Alpine Wetland in the Source of Yellow River [J]. Acta Agrestia Sinica, 2021, 29(7): 1540-1548.
[3]	YANG Yang, ZHANG Ni, JIANG Li-li, CHEN Ke-long. Effects of Simulated Precipitation on Soil Edaphic Physicochemical Factors and Microbial Community Characteristics in Bird Island of Qinghai Lake on The Tibetan Plateau [J]. Acta Agrestia Sinica, 2021, 29(5): 1043-1052.
[4]	DING Cheng-xiang, LIU Yu. Effects of Solar Photovoltaic Park Construction on Soil Microbial Community in Alpine Desert of Qinghai Tibet Plateau [J]. Acta Agrestia Sinica, 2021, 29(5): 1061-1069.
[5]	YU Lan-hui, WANG Jie, LIAO Li-rong, ZHANG Chao, LIU Guo-bin. Soil Microbial Biomass,Enzyme Activities and Ecological Stoichiometric Characteristics and Influencing Factors along Degraded Meadows on the Qinghai-Tibet Plateau [J]. Acta Agrestia Sinica, 2020, 28(6): 1702-1710.
[6]	DING Cheng-xiang, YANG Xiao-xia, Dong Quan-min. Effects of Grazing Patterns on Vegetation,Soil and Microbial Community in Alpine Grassland of Qinghai-Tibetan Plateau [J]. Acta Agrestia Sinica, 2020, 28(1): 159-169.
[7]	MA Yuan, LI Ling-zhi, ZHANG De-gang, YANG Jie, YAO Yu-jiao, CHEN Jian-gang. Distribution Characteristics of Nutrients and Microbial Biomass in Rhizosphere and Non-rhizosphere Soils of Dominant Plants in Degraded Alpine Meadow [J]. Acta Agrestia Sinica, 2019, 27(4): 797-804.
[8]	QIN Fu-wen, KANG Bin-yue, JIANG Feng-yan, XU Heng-kang, ZHOU Hua-kun, WEI Xiao-ting, LIU Xiao-li, SHAO Xin-qing. Effects of Biological Crusts Succession on Soil Microbial Communities in Alpine Steppe [J]. Acta Agrestia Sinica, 2019, 27(4): 832-840.
[9]	JIANG Lu-fan, LI Ya-heng, YANG Jin-rong, WANG Yong. Effect of Precipitation Change on the Quantity of Soil Fauna in Xilingol Grassland [J]. Acta Agrestia Sinica, 2019, 27(3): 766-774.
[10]	NAN Li-li, WANG Kun, LI Xiao-yan, ZHAO Ke-ming. Effects of Seeding Rate and Row Spacing on Soil Biological Properties in the Rhizosphere of Alfalfa [J]. Acta Agrestia Sinica, 2018, 26(6): 1330-1336.
[11]	CHEN Dong-dong, LI Qi, CHEN Xin, HE Fu-quan, ZHAO Xin-quan, XU Shi-xiao, ZHAO Liang. Response of Soil Microbial Biomass C and N,C Metabolism Characteristics of Microbes to Grass-Legume Mixtures of Annual Artificial Grassland in Sanjiangyuan Region [J]. Acta Agrestia Sinica, 2018, 26(5): 1064-1070.
[12]	WU Jia-hua, WANG Li-xin, ZHANG Jing-hui, ZHUO Yi, WU Sheng-nan, WANG Feng-ge, XU Zhi-chao, QI Yu, WEN Lu. Response of Soil Properties and Microbial Biomass to Different Grazing Intensities in Temperate Typical Steppe [J]. Acta Agrestia Sinica, 2018, 26(4): 832-840.
[13]	QI Yong-hong, CAO Su-fang, LI Xue-ping, LI Min-quan. Study on the Relationship between Root Rot Disease and Nutrient Content,Enzyme Activity and Microbial Quantity in Rhizosphere Soil of Naked Barley in Lintan County,Gannan State [J]. Acta Agrestia Sinica, 2018, 26(4): 877-884.
[14]	YU Hong-qian, JIANG Qi, WANG Zhan-jun, HE Jian-long, HE Chen. Application of VOR and CVOR Index for Health Assessment of Desert Steppe in Ningxia-Taking Yanchi County for Example [J]. Acta Agrestia Sinica, 2018, 26(3): 584-590.
[15]	ZHANG Xiao-lin, ZHAI Peng-hui, HUANG Jian-hui. Advances in the Influences of Precipitation and Nitrogen Deposition Change on the Carbon Cycle of Grassland Ecosystem [J]. Acta Agrestia Sinica, 2018, 26(2): 284-288.