Effects of Biogas Slurry Application on the Soil Microorganisms of P.americanum × P.purpureum Grassland

doi:10.11733/j.issn.1007-0435.2016.01.008

Acta Agrestia Sinica ›› 2016, Vol. 24 ›› Issue (1): 54-60.DOI: 10.11733/j.issn.1007-0435.2016.01.008

Effects of Biogas Slurry Application on the Soil Microorganisms of P.americanum × P.purpureum Grassland

ZHONG Zhen-mei^1,2, SONG Ya-na³, HUANG Xiu-sheng^1,2, YOU Xiao-feng^1,2, WENG Bo-qi^1,2, HUANG Qin-lou^2,4, CHEN Zhong-dian^1,2, FENG De-qing^1,2

1. Agricultural Ecology Institute, Fujian Academy of Agricultural Sciences (FAAS), Fuzhou, Fujian Province 350003, China;
2. Fujian Engineering and Technology Research Center for Hilly Prataculture, Fuzhou, Fujian Province 350003, China;
3. Institute of biotechnology, FAAS, Fuzhou, Fujian Province 350003, China;
4. Institute of Animal Husbandry and Veterinary Medicine, FAAS, Fuzhou, Fujian Province 350003, China

Received:2015-02-07 Revised:2015-07-07 Online:2016-02-15 Published:2016-04-26

沼液对狼尾草地土壤微生物群落的影响

钟珍梅^1,2, 宋亚娜³, 黄秀声^1,2, 游小凤^1,2, 翁伯琦^1,2, 黄勤楼^2,4, 陈钟佃^1,2, 冯德庆^1,2

1. 福建省农业科学院农业生态研究所, 福建福州 350003;
2. 福建省丘陵地区循环农业工程技术研究中心, 福建福州 350003;
3. 福建省农业科学院生物技术研究所, 福建福州 350003;
4. 福建省农业科学院畜牧兽医研究所, 福建福州 350003

通讯作者: 翁伯琦
作者简介:钟珍梅(1975-),女,畲族,博士,副研究员,主要从事农业生态和污染生态研究,E-mail:mume19@126.com
基金资助:
国家科技支撑项目(2012BAD14B15,2011BAD17B02);福建省公益类科研院所基本科研专项(2014R1017-7);福建省水土保持重大专项(2012NZ0002)资助

Abstract

Abstract:

In order to reveal the effects of biogas slurry application on improving soil fertility, the change of soil microorganisms under different amounts of biogas slurry (equal to 200, 150, 100 and 50 kg N·ha^-2pure N) application on Pennisetum.americanum × Pennisetum.purpureum grassland was studied by chloroform fumigation, fluorescence quantitative PCR and PCR-DGGE methods based on field experiment. The results showed that soil microbial biomass C and N of 4 treatments significantly increased by biogas slurry application compared to control, and there was a significant correlation between the slurry application amount and the increment of soil microbial biomass C and N. Biogas slurry significantly increased the abundance of 16S rDNA and SSU rDNA gene which was significantly related with the application amount of biogas slurry, and the quantities of 16S rDNA-containing bacterial and SSU rDNA-containing fungi were the maximum in the treatment of containing 200 kg N·ha^-2 biogas slurry, while the abundance of nifH -containing gene significantly decreased. As a result, it could conclude that the application of biogas slurry has no significant effect on the community diversity of soil bacteria, fungi and nitrogen-fixing bacteria, but promotes the growth of microorganisms containing 16S rDNA gene and SSU rDNA genes, inhibits bacterial growth containing nifH gene, and changes the number of microorganisms.

Key words: Biogas slurry, Bacterial, Fungi, Azotobacter, Gene abundance, Microbial community diversity

摘要：

为探明沼液浇灌对土壤肥力及微生物的影响,揭示沼液提高土壤肥力的机制,本文采用氯仿熏蒸法、荧光定量PCR(fluorescence quantitative PCR)和PCR-DGGE法研究不同梯度沼液浇灌量(折纯N量200,150,100,50kgN·hm^-2)对狼尾草(P.americanum×P.purpureum)地土壤微生物的影响。结果表明,与对照相比,沼液浇灌能显著提高土壤微生物生物量N和C,且沼液浇灌量与微生物量N和C的增量显著相关。沼液浇灌还能显著增加细菌16SrDNA基因和真菌SSUrDNA基因丰度,其中200kgN·hm^-2处理含16SrDNA基因和SSUrDNA基因的微生物数量最多,沼液浇灌量与土壤中含16SrDNA基因和SSUrDNA基因的微生物数量呈显著正相关,但显著降低固氮菌nifH的基因丰度。沼液浇灌人工草地对包括细菌、真菌和固氮菌在内的土壤微生物群落多样性无显著影响,可促进含16SrDNA基因和SSUrDNA基因的微生物繁殖,但在一定程度上会抑制含nifH基因的微生物的繁殖,改变土壤含16SrDNA基因、SSUrDNA基因和nifH基因的微生物数量。

关键词: 沼液, 细菌, 真菌, 固氮菌, 基因丰度, 微生物多样性

CLC Number:

S182

ZHONG Zhen-mei, SONG Ya-na, HUANG Xiu-sheng, YOU Xiao-feng, WENG Bo-qi, HUANG Qin-lou, CHEN Zhong-dian, FENG De-qing. Effects of Biogas Slurry Application on the Soil Microorganisms of P.americanum × P.purpureum Grassland[J]. Acta Agrestia Sinica, 2016, 24(1): 54-60.

钟珍梅, 宋亚娜, 黄秀声, 游小凤, 翁伯琦, 黄勤楼, 陈钟佃, 冯德庆. 沼液对狼尾草地土壤微生物群落的影响[J]. 草地学报, 2016, 24(1): 54-60.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2016.01.008

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2016/V24/I1/54

References

[1] 张树清,张夫道,刘秀梅,等. 规模化养殖畜禽粪主要有害成分测定分析研究[J]. 植物营养与肥料学报,2005,11(6):822-829
[2] 李丙智,王桂芳,秦晓飞,等. 沼液配施钾肥对果园土壤理化特性和微生物及果实品质影响[J]. 中国农业科学,2010,43(22):4671-4677
[3] 耿晨光,段婧婧,李汛,等. 沼液的园林地消解处理利用及其对土壤微生物碳、氮与酶活性的影响[J]. 农业环境科学学报,2012,31(10):1965-1971
[4] 杨青华,韩锦峰. 棉田不同覆盖方式对土壤微生物和酶活性的影响[J]. 土壤学报,2005,42(2):348-351
[5] 付国占,李潮海,王俊忠,等. 残茬覆盖与耕作方式对土壤性状及夏玉米水分利用效率的影响[J]. 农业工程学报,2005,21(1):52-56
[6] Henriksen T M,Breland T A.Carbon mineralization,fungal and bacterial growth and enzyme activities as affected by contact between crop residues and soil[J].Biology and Fertility of Soils,2002,35(1):41-48
[7] 管涛,冯伟,王化岑,等. 追施沼液对冬小麦根际土壤生物活性的影响[J]. 麦类作物学报,2010,30(4):721-726
[8] 张玉平,刘强,荣湘民,等. 不同有机肥与化肥配施对稻田土壤微生物活性的影响[J]. 土壤通报,2013,44(6):1434-1439
[9] 郝鲜俊,洪坚平,乔志伟. 沼液对甘蓝连作土壤生物学性质的影响[J]. 应用与环境生物学报,2011,17(3):384-387
[10] 冯伟,管涛,王晓宇,等. 沼液与化肥配施对冬小麦根际土壤微生物数量和酶活性的影响[J]. 应用生态学报,2011,22(4):1007-1012
[11] 刘文科,杨其长,王顺清. 沼液在蔬菜上的应用及其土壤质量效应[J]. 中国沼气,2009,27(1):43-47
[12] Abubaker J, Cederlund H, Arthurson V, et al. Bacterial community structure and microbial activity in different soils amended with biogas residues and cattle slurry[J]. Applied Soil Ecology,2013,72(10):171-180
[13] 曹云,常志州,马艳,等. 沼液施用对辣椒疫病的防治效果及对土壤生物学特性的影响[J]. 中国农业科学,2013,46(3):507-516
[14] 鲁如坤. 土壤农业化学分析方法[M]. 北京:中国农业科技出版社,2000
[15] 林先贵. 土壤微生物研究原理与方法[M]. 北京:高等教育出版社,2010:73
[16] He J Z, Shen J P, Zhang L M, et al. Quantitative analyses of the abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea of a Chinese upland red soil under long-term fertilization practices[J]. Environmental Microbiology,2007,9(9):2364-2374
[17] Muyzer G, De Waal E C, Uitterlinden A G. Profiling of complex microbial population by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA[J]. Applied and Environmental Microbiology,1993,59(3):695-700
[18] Vainio E J, Hantula J. Direct analysis of wood-inhabiting fungi using denaturing gradient gel electrophoresis of amplified ribosomal DNA[J]. Mycological Research,2000,104(8):927-936
[19] Poly F, Monrozier L J, Bally R. Improvement in the RFLP procedure for studying the diversity of nifH genesin communities of nitrogen fixers in soil[J]. Research in Microbiology,2001,152(1):95-103
[20] Zak J C, Willig M R, Moorhead D L, et al. Functional diversity of microbial communities: a quantitative approach[J]. Soil Biology & Biochemistry,1994,26(9):1101-1108
[21] Jenkinson D S, Ladd J N. Microbial biomass in soil: Measurement and turnover[M]. New York,1981:415-471
[22] Allison S D, Martiny J B H. Resistance, resilience, and redundancy in microbial communities[J]. Proceedings of the National Academy of Sciences of the USA,2008,105(S1):11512-11519
[23] 周桔,雷霆. 土壤微生物多样性影响因素及研究方法的现状与展望[J]. 生物多样性,2007,15(3):306-311
[24] 李娜,张利敏,张雪萍. 土壤微生物群落结构影响因素的探讨[J]. 哈尔滨师范大学自然科学学报,2012,28(6):70-74
[25] Geisseler D, Scow K M. Long-term effects of mineral fertilizers on soil microorganisms-A Review[J]. Soil Biology & Biochemistry,2014,75(8):54-63
[26] 王远远,刘荣厚. 沼液综合利用研究进展[J]. 安徽农业科学,2007,35(4):1089-1091
[27] Coelho M R R, Marriel I E, Jenkins S N, et al.Molecular detection and quantification ofnifH gene sequences in the rhizosphere of sorghum ( Sorghum bicolor) sown with two levels of nitrogen fertilizer[J]. Applied Soil Ecology,2009,42(1):48-53
[28] Ogilvie L A, Hirsch P R, Johnston A W. Bacterial diversity of the Broadbalk 'classical' winter wheat experiment in relation to long-term fertilizer inputs[J]. Microbial Ecology,2008,56(3):525-537
[29] Juraeva D, George E, Davranov K, et al.Detection and quantification of the nifH gene in shoot and root of cucumber plants[J]. Canadian Journal of Microbiology,2006,52(8):731-739
[30] 张于光,王慧敏,李迪强,等. 三江源高寒草甸土固氮基因(nifH) 的多样性和系统发育研究[J]. 微生物学报,2005,45(2):166-171
[31] 董志新,孙波,殷士学,等. 气候条件和作物对黑土和潮土固氮微生物群落多样性的影响[J]. 土壤学报,2012,49(1):130-138
[32] Shu W, Pablo G P, Jun Y, et al.Abundance and diversity of nitrogen-fixing bacteria in rhizosphere and bulk paddy soil under different duration of organic management[J]. World Journal of Microbiology and Biotechnology,2012,28(2):493-503

Effects of Biogas Slurry Application on the Soil Microorganisms of P.americanum × P.purpureum Grassland

沼液对狼尾草地土壤微生物群落的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	HUANG Cai-xia, LU Guang-xin, LI Xin, FAN Yue-jun, ZHOU Hua-kun, WANG Ying-cheng, WANG Zhi-hui, YAO Shi-ting. Effect of a Laccase Producing Fungus on the Mineralization Characteristics of Soil Organic Matter in Alpine Meadow [J]. Acta Agrestia Sinica, 2021, 29(S1): 173-178.
[2]	SHI Guo-xi, WANG Fang-ping, MA Li, ZHANG Zhong-hua, WANG Yi-bo, WANG Zhi-bo, YAO Bu-qing, ZHOU Hua-kun. Effects of Long-term and Short-term Warming on Arbuscular Mycorrhizal Fungal Community in Alpine Meadow Ecosystem [J]. Acta Agrestia Sinica, 2021, 29(S1): 179-189.
[3]	WANG Tan-guo-yan, MA Zhi-yuan, LI Pei-yang, GUO Jun-hong, ZHANG Jia-yi, JIANG Sheng-jing. Effects of Short-Term Warming on Arbuscular Mycorrhizal Fungi in the Rhizosphere of Different Plant Species in Alpine Meadows on the Qinghai-Tibetan Plateau [J]. Acta Agrestia Sinica, 2021, 29(9): 1959-1966.
[4]	TANG Li-hui, LIU Yi-ling, ZHAO Wen-xing, ZHANG Yun-hao, WU Ke-xin, GUO Zi-yu, MO Chong-hui, ZHAO Bao-yu, LU Hao. Isolation,Identification and Tissue Distribution Characteristics of Fungal Endophyte in Ligularia sagitta [J]. Acta Agrestia Sinica, 2021, 29(8): 1658-1665.
[5]	ZHENG Min-na, LIANG Xiu-zhi, HAN Zhi-shun, KANG Jia-hui, CHEN Yan-ni. Effects of Different Improvement Measures on the Diversity of Soil Bacteria Communities in Salt-alkali Soil [J]. Acta Agrestia Sinica, 2021, 29(6): 1200-1209.
[6]	SUN Lei-lei, ZHAO Gui-qin, CHAI Ji-kuan, LIU Fu-yuan, Nie Xiumei, WANG Jun. Effects of Storage Methods and Time on Mould Quantity and Species in Alfalfa Hay [J]. Acta Agrestia Sinica, 2021, 29(5): 884-893.
[7]	WANG Xin-yu, SHEN Chen, HUO Wen-jie, LIU Qiang, ZHANG Shuan-lin, WANG Cong, CHEN Lei, XU Qing-fang, WANG Yong-xin, GUO Gang. Analysis of Fermentation Quality and Bacterial Community Structure of Whole Corn Silage of Different Varieties in Yanbei Region [J]. Acta Agrestia Sinica, 2021, 29(5): 1087-1093.
[8]	PAN Yue, ZHOU Ji-qiong, GUO Chuan, YANG Zhe, LIU Lin, YAN Yan-hong, ZHAO Yan-lan, LIU Shan, SUN Fei-da. Arbuscular Mycorrhizal Fungi and Rhizobia Regulate Plant Interspecific Interaction of Three Legumes and Grasses Species [J]. Acta Agrestia Sinica, 2021, 29(4): 644-654.
[9]	LU Yong-xiang, YAN Xian-ming, ZHOU Zhao-xiang, ZHU Hui-xian, GOU Wen-long, YOU Ming-hong, LI Da-xu, CHEN Shi-yong, LI Ping, BAI Shi-qie. Effects of Different Additives on Fermentation Quality and Bacterial Community of High-moisture Whole-plant Corn Silage [J]. Acta Agrestia Sinica, 2021, 29(4): 842-847.
[10]	SU Bei-bei, ZHANG Ying, DAO Ri-na. Study on the Distribution Characteristics of Bacterial Communities in the Rhizosphere Soil of Four Leguminous Cultivated Forages [J]. Acta Agrestia Sinica, 2021, 29(2): 250-258.
[11]	LIU Chong-yi, JIN Xu-mei, WANG Ying-ying, LI Teng-fei, CHEN Xin-yi, CAO Xin-yue, LONG Ming-xiu, HE Shu-bin. Response of Soil Characteristics and Bacterial Communities to Cover Crops in Organic Kiwifruit Orchard in Guanzhong Plain,China [J]. Acta Agrestia Sinica, 2021, 29(12): 2711-2720.
[12]	ZHANG Hai-juan, LU Guang-xin, FAN Yue-jun, ZHOU Hua-kun, ZHOU Xue-li, DOU Sheng-yun, YAN Hui-lin, MA Kun, ZHAO Yang-an. Effect of Different Dying Methods on the Mycorrhizal Infection Rate of Two Gramineous Forages [J]. Acta Agrestia Sinica, 2021, 29(12): 2838-2844.
[13]	QI Chen-xi, YI Guan-tao, FU Xiao-xuan, WANG Hai-long, WANG Jia-lin, WU Jian-hui. Effects of Arbuscular Mycorrhizal Fungi on Physiological Indexes of Potentilla anserina under Drought-Stress [J]. Acta Agrestia Sinica, 2021, 29(11): 2407-2412.
[14]	NAN Li-li, GUO Quan-en, LIU Xue-qiang. Effects of the Alfalfa Post-harvest Rotation on Soil Bacterial Community in Arid Desert Oasis [J]. Acta Agrestia Sinica, 2021, 29(1): 25-32.
[15]	XIE Kai-yun, SUN ling-li, ZHANG Li-wen, ZHENG Wan-ju, WAN Jiang-chun, ZHAO Yun. Effect of Arbuscular Mycorrhizal Fungi and Rhizobium Inoculation on the Biomass of Alfalfa and Smooth Brome in Mixed Culture [J]. Acta Agrestia Sinica, 2021, 29(1): 182-188.