Identification of the Related microRNA of Cytoplasmic Male Sterile Line of Alfalfa

doi:10.11733/j.issn.1007-0435.2019.05.004

Acta Agrestia Sinica ›› 2019, Vol. 27 ›› Issue (5): 1138-1146.DOI: 10.11733/j.issn.1007-0435.2019.05.004

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Identification of the Related microRNA of Cytoplasmic Male Sterile Line of Alfalfa

GUO Qiang¹, WANG Ying-zhe², CHEN Jing-jing¹, ZHOU Le¹, XU Bo¹

1. College Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province 130118, China;
2. Jilin Academy of Agricultural Sciences, Changchun, Jilin Province 130033, China

Received:2019-04-28 Revised:2019-07-04 Online:2019-10-15 Published:2019-11-09

紫花苜蓿细胞质雄性不育系相关microRNA的鉴定

郭强¹, 王英哲², 陈晶晶¹, 周仂¹, 徐博¹

1. 吉林农业大学动物科学技术学院, 吉林长春 130118;
2. 吉林省农业科学院, 吉林长春 130033

通讯作者: 徐博
作者简介:郭强(1993-),男,黑龙江大庆人,硕士研究生,主要从事牧草种质资源开发与利用研究,E-mail:278315633@qq.com
基金资助:
吉林省科技厅科技支撑计划“优质饲草品种选育及其配套种植技术研究与示范”（20180201072ny）；吉林省教育厅科学技术研究项目“紫花苜蓿细胞质雄性不育系的细胞学和生理生化特性的研究资助”（JJKH20180666KJ）共同资助

Abstract

Abstract: Alfalfa (Medicago sativa L.) is a superior leguminous herbage. It is important to use cytoplasmic male sterile line of alfalfa for seed production. The identification of related microRNA is an important part of the research on its molecular mechanism. In this study,the buds of the cultivated cytoplasmic male sterile line MSGN1A and its homologous maintainer line MSGN1B of alfalfa were used as experimental materials. The differentially expressed microRNA and target genes bioinformatics of the MSGN1A and MSGN1B of alfalfa at anther development stage were studied using small RNA sequencing techniques. Through identification,1 478 miRNAs were detected in this study,including 1 351 known miRNAs and 127 novel miRNAs. A total of 130 differentially expressed microRNAs were screened,including 90 up-regulated miRNAs and 40 down-regulated microRNAs. Differentially expressed miRNA target genes results showed that the major metabolic pathways included "metabolic process","cell process","signal biological process","plant hormone signaling","oxidative phosphorylation",and "starch and sucrose metabolism". The differentially expressed microRNAs had the function of target genes to regulate the development of anthers,followed by the regulation of flowering and signal transduction,while the differentially expressed microRNA-targeted transcription factors were mostly factors controlling the processes of flowering,anther development and signal transduction. The abortion of the breeding system had a certain impact.

Key words: Medicago sativa L., Cytoplasmic male sterility, Differentially expressed microRNA, MicroRNA target genes

摘要： 紫花苜蓿（Medicago sativa L.）是一种多年生豆科苜蓿属牧草，利用紫花苜蓿细胞质雄性不育系育种有着重要意义，相关microRNA的鉴定工作是研究其分子机制的重要内容。本试验以培育的紫花苜蓿细胞质雄性不育系MSGN1A及其同型保持系MSGN1B的花蕾为试验材料，采用small RNA测序技术对MSGN1A及MSGN1B在花药发育阶段的鉴定差异表达microRNA以及差异表达microRNA靶基因功能进行注释。结果表明：共计检测到1 478个miRNA，其中known miRNA 1 351个，novel miRNA 127个；筛选出差异表达miRNA 130个，包括90个上调表达的miRNA和40个下调表达的miRNA；差异表达micro RNA靶基因主要涉及的通路有：“代谢过程”、“细胞过程”、“信号生物过程”、“植物激素信号传导”、“氧化磷酸化”和“淀粉蔗糖代谢”等。差异表达microRNA靶基因的功能主要是调控花药的发育，其次是调控花期以及信号传导，而差异表达microRNA靶向的转录因子中多为控制花期、花药发育和信号传导等过程的因子，对不育系的败育起到一定的影响。

关键词: 紫花苜蓿, 细胞质雄性不育, 差异表达microRNA, microRNA靶基因

CLC Number:

S541

GUO Qiang, WANG Ying-zhe, CHEN Jing-jing, ZHOU Le, XU Bo. Identification of the Related microRNA of Cytoplasmic Male Sterile Line of Alfalfa[J]. Acta Agrestia Sinica, 2019, 27(5): 1138-1146.

郭强, 王英哲, 陈晶晶, 周仂, 徐博. 紫花苜蓿细胞质雄性不育系相关microRNA的鉴定[J]. 草地学报, 2019, 27(5): 1138-1146.

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Identification of the Related microRNA of Cytoplasmic Male Sterile Line of Alfalfa

紫花苜蓿细胞质雄性不育系相关microRNA的鉴定

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