Transcriptomic Analysis of Anthocyanin Synthesis in Alfalfa

doi:10.11733/j.issn.1007-0435.2021.05.002

Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (5): 866-875.DOI: 10.11733/j.issn.1007-0435.2021.05.002

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Transcriptomic Analysis of Anthocyanin Synthesis in Alfalfa

PAN Xin-yi^1,2, SHI Kun², GONG Pan¹, WEI Bao¹, WU Xin-ming³, WANG Zan²

1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China;
3. Institute of Animal Sciences, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi Province 030032, China

Received:2021-02-25 Revised:2021-03-18 Online:2021-05-15 Published:2021-06-02

紫花苜蓿花青苷合成的转录组分析

潘新怡^1,2, 史昆², 龚攀¹, 韦宝¹, 吴欣明³, 王赞²

1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
2. 中国农业大学草业科学与技术学院, 北京 100193;
3. 山西省农业科学院畜牧兽医研究所, 山西太原 030032

通讯作者: 王赞,E-mail:zanwang@cau.edu.cn
作者简介:潘新怡(1994-)，女，河北廊坊人，硕士研究生，主要从事牧草基因资源发掘与利用方面的研究，E-mail：panxinyi19@163.com
基金资助:
国家自然科学基金（31761143013）；国家林业和草原种质资源库（2005DKA21003）资助

Abstract

Abstract: To explore the molecular mechanism of flower color formation in alfalfa,this experiment used alfalfa and its white-flowered mutants as research materials and performed transcriptome sequencing analysis. The results showed that there were 4 857 differentially expressed genes(DGEs). The metabolic pathway enrichment analysis showed that six metabolic pathways,including phenylalanine biosynthesis pathway,were significantly enriched; The study also found that the expression of Dihydroflavonol 4-reductase (DFR),a key gene in the flavonoid synthesis pathway,was significantly down-regulated in the white flower mutant， there were binding sites of MYB and bHLH in its promoter; The results of transcriptome and real-time PCR showed that the transcription factor family genes such as MYB,bHLH and WD40 were differentially expressed. These results indicated that MYB transcription factor or MBW complex may affect the formation of alfalfa flower color by regulating the transcription of DFR. This study provided a theoretical basis for the study of the molecular mechanism of alfalfa anthocyanin synthesis.

Key words: Medicago sativa, RNA-Seq, Anthocyanins, Transcriptional regulation

摘要： 为探究紫花苜蓿（Medicago sativa）花色形成的分子机制，本试验以紫花苜蓿及其白花突变体为研究材料，进行转录组测序。结果表明：紫花和白花之间共有差异表达基因4 857个；代谢通路富集分析中，苯丙氨酸生物合成途径等6条代谢通路显著富集；研究还发现类黄酮合成通路中关键基因二氢黄酮醇4-还原酶（Dihydroflavonol 4-reductase，DFR）在白花突变体中的表达量显著下调，其启动子中存在MYB和bHLH等转录因子的结合位点；转录组以及荧光定量PCR的结果均表明：MYB，bHLH和WD40等基因的表达量都具有显著性差异。这些结果表明MYB转录因子或MBW复合物可能通过调控DFR的转录影响紫花苜蓿花色形成。本试验为探明紫花苜蓿花青苷合成的分子机制提供了理论基础。

关键词: 紫花苜蓿, 转录组, 花青苷, 转录调控

CLC Number:

S963.22+3.3

PAN Xin-yi, SHI Kun, GONG Pan, WEI Bao, WU Xin-ming, WANG Zan. Transcriptomic Analysis of Anthocyanin Synthesis in Alfalfa[J]. Acta Agrestia Sinica, 2021, 29(5): 866-875.

潘新怡, 史昆, 龚攀, 韦宝, 吴欣明, 王赞. 紫花苜蓿花青苷合成的转录组分析[J]. 草地学报, 2021, 29(5): 866-875.

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Transcriptomic Analysis of Anthocyanin Synthesis in Alfalfa

紫花苜蓿花青苷合成的转录组分析

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