Acta Agrestia Sinica ›› 2014, Vol. 22 ›› Issue (6): 1252-1257.DOI: 10.11733/j.issn.1007-0435.2014.06.016

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Identification of Endophytic Bacteria from Kobresia humilis and Determination of Phosphate-solubilizing, IAA Secretion and Antagonistic Abilities

YAO Yu-ling, WANG Ying, WANG Yu-qin, YANG Cheng-de   

  1. Key Laboratory of Grassland Ecosystem, Ministry of Education, College of Pratacultural Science, Gansu Agricultural University, Pratacultural Engineering Laboratory of Gansu Province, Sino-U.S. Centers for Grazingland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China
  • Received:2014-01-06 Revised:2014-04-09 Online:2014-12-15 Published:2014-12-01

矮生嵩草内生细菌溶磷、抑菌和产IAA能力的测定及鉴定

姚玉玲, 王颖, 王玉琴, 杨成德   

  1. 草业生态系统教育部重点实验室 甘肃农业大学 甘肃省草业工程实验室 中-美草地畜牧业可持续发展研究中心, 甘肃 兰州 730070
  • 通讯作者: 杨成德
  • 作者简介:姚玉玲(1988-),女,湖北宜昌人,硕士研究生,主要从事植物病理学的研究,E-mail:1016456192@qq.com
  • 基金资助:

    国家自然科学基金(31160122);草业生态系统教育部重点实验室(甘肃农业大学)开放课题(CYZS-2011011)资助

Abstract:

The studies focused on endophytic bacteria 263AG5 from Kobresia humilis in alpine grassland near the Eastern Qilian Mountains. The methods of dual culture and Salkowski colorimetric were used to determine the antagonistic effect and IAA secretion ability of endophytic bacteria. Strain 263AG5 was identified by morphological and molecular biological methods. The results showed that 263AG5 inhibited the pathogenic fungi of potato, such as Phoma foveata and Colletotrichum coccodes, and had the abilities of nitrogen fixation and IAA secretion. The phosphorus solubilizing ability of 263AG5 was 60.52 mg·L-1. Endophytic bacteria 263AG5 was rod-shaped with a size of (0.71~1.78)×(0.27~0.48) μm, Gram-positive. The sequence analyses of 16S rDNA gene showed that 263AG5 was Bacillus mojavensis. Therefore, 263AG5 could be developed as microbial fungicide.

Key words: Kobresia humilis, Biological function, Endophytic bacteria, Identification

摘要:

以东祁连山高寒草地矮生嵩草(Kobresia humilis)内生细菌263AG5为研究对象,采用平板对峙法和Salkowski比色法测定其抑菌和分泌植物生长素(IAA)的能力,并利用形态学和分子生物学方法对其进行鉴定.结果表明:263AG5对马铃薯坏疽病菌(Phoma foveata)和炭疽病菌(Colletotrichum coccodes)均有较好的抑制作用,且具有固氮和产IAA的能力,溶磷量为60.52 mg·L-1.263AG5菌体杆状,大小为(0.71~1.78)×(0.27~0.48) μm,呈革兰氏阳性,结合16S rDNA基因序列相似性分析鉴定263AG5为莫海威芽孢杆菌(Bacillus mojavensis),该菌具有开发为微生物农药的潜力.

关键词: 矮生嵩草, 生物功能, 内生细菌, 鉴定

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