Effects of Exogenous Hormone Treatment on the Growth and Development of Rihzome of Caucasian Clover

doi:10.11733/j.issn.1007-0435.2026.03.017

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (3): 929-939.DOI: 10.11733/j.issn.1007-0435.2026.03.017

Effects of Exogenous Hormone Treatment on the Growth and Development of Rihzome of Caucasian Clover

YANG Ming-hao¹, LONG Xi¹, Yan Xu¹, JIANG Ding-yu¹, WANG Zhao-ming², ZHAO Si-wen¹, NIE Wan-ting¹, JIANG Jing-wen¹, WANG Zi-cheng¹, CHEN Zi-rui¹, YANG Zi-yuan¹, YIN Xiu-jie¹

1. Northeast Agricultural University, Harbin, Heilongjiang Province 150006, China;
2. National Grassland Technology Innovation Center (Provisional), Hohhot, Inner Mongolia 010000, China

Received:2025-02-10 Revised:2025-04-23 Published:2026-03-23

外源激素处理对高加索三叶草根蘖芽生长发育的影响

杨明浩¹, 龙羲¹, 闫旭¹, 姜丁予¹, 王召明², 赵思文¹, 聂婉婷¹, 姜婧雯¹, 王孜成¹, 陈子瑞¹, 杨子源¹, 殷秀杰¹

1. 东北农业大学, 黑龙江哈尔滨 150006;
2. 国家草业技术创新中心(筹), 内蒙古呼和浩特 010000

通讯作者: 殷秀杰，E-mail：yinxiujie@126.com
作者简介:杨明浩（2001-），男，汉族，黑龙江鸡西人，硕士研究生，主要从事草种质资源与育种研究，E-mail：13763669989@163.com；
基金资助:
国家草业技术创新中心（筹）重大创新平台建设专项（CCPTZX2023N04，CCPTZX2024GJ01-1-2）；国家自然科学基金项目（32471760）；国家重点研发计划（2023YFD1200302）；黑龙江省联合重点基金（ZL2024C018）资助

Abstract

Abstract: Plant hormones play important regulatory roles in crop growth， but the relationship and mechanism by which exogenous hormones induce the growth and development of Caucasian clover root tillers are still unclear. In this study， the effects of indole acetic acid （IAA）， cytokinin （CTK） and kinetin （KT） on the growth and development， endogenous hormone content and expression of related genes in Caucasian clover root tillers were compared. The results showed when the concentration of IAA was 8.0 mg·L^-1， exogenous IAA treatment had the strongest effect on the growth of Caucasian clover root tillers. And when CTK concentration was 100.0 mg·L^-1， exogenous CTK treatment had the strongest inhibitory effect on the growth of Caucasian clover. Exogenous KT treatment had the greatest effect on the growth of Caucasian clover root tillers while KT concentration was 0.5 mg·L^-1. Compared with the control group， exogenous hormone treatment had a significant effect on BRI1， AP2/ERF， KNOX， MAPK gene family， PAL， POD and other related genes. In conclusion， 8.0 mg·L^-1 IAA and 0.5 mg·L^-1 KT work best. This study provides a practical guidance for the artificial propagation and rapid grassland planting of Caucasian clover.

Key words: Caucasian clover, Plant hormone, Complex hormone, Gene family

摘要： 植物激素在作物生长过程中起着重要的调节作用，然而外源激素诱导高加索三叶草（Trifolium ambiguum）根蘖芽生长发育的关系和机制尚不清楚。本研究比较了添加外源生长素（Indole acetic acid，IAA）、细胞分裂素（Cytokinin， CTK）和激动素（Kinetin，KT）对高加索三叶草根蘖芽生长发育、内源激素含量以及相关基因表达的影响，并选取对植株有正向响应的IAA和KT进行复合处理，以研究其对根蘖芽生长的影响。结果表明：IAA浓度为8.0 mg·L^-1时，外源IAA处理对高加索三叶草根蘖芽生长促进作用最强；CTK浓度为100.0 mg·L^-1时，外源CTK处理对高加索三叶草生长抑制作用最强；KT浓度为0.5 mg·L^-1时，外源KT处理对高加索三叶草根蘖芽生长影响最大。外源激素通过影响内源激素含量来调控植株生长发育，与对照组相比，外源激素处理对BRI1，AP2/ERF，KNOX，MAPK基因家族和PAL，POD等相关基因有着显著影响。复合激素中8.0 mg·L^-1 IAA与0.5 mg·L^-1 KT效果最佳。本研究为高加索三叶草的人工扩繁和草地快速建植提供了实践指导依据。

关键词: 高加索三叶草, 植物激素, 复合激素, 基因家族

CLC Number:

S541.2

YANG Ming-hao, LONG Xi, Yan Xu, JIANG Ding-yu, WANG Zhao-ming, ZHAO Si-wen, NIE Wan-ting, JIANG Jing-wen, WANG Zi-cheng, CHEN Zi-rui, YANG Zi-yuan, YIN Xiu-jie. Effects of Exogenous Hormone Treatment on the Growth and Development of Rihzome of Caucasian Clover[J]. Acta Agrestia Sinica, 2026, 34(3): 929-939.

杨明浩, 龙羲, 闫旭, 姜丁予, 王召明, 赵思文, 聂婉婷, 姜婧雯, 王孜成, 陈子瑞, 杨子源, 殷秀杰. 外源激素处理对高加索三叶草根蘖芽生长发育的影响[J]. 草地学报, 2026, 34(3): 929-939.

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Effects of Exogenous Hormone Treatment on the Growth and Development of Rihzome of Caucasian Clover

外源激素处理对高加索三叶草根蘖芽生长发育的影响

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