Evaluation of Production Performance and Fingerprint Construction of Pennisetum Varieties

doi:10.11733/j.issn.1007-0435.2026.10.016

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (10): 3693-3703.DOI: 10.11733/j.issn.1007-0435.2026.10.016

Evaluation of Production Performance and Fingerprint Construction of Pennisetum Varieties

ZUO Su-tian, YU Shuai, ZHANG Xian-fang, NIE Gang, JIANG Zhe-ji, PENG Yan, ZHANG Xin-quan

College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan Province 611130, China

Received:2026-02-09 Revised:2026-06-26 Published:2026-10-10

狼尾草品种（系）生产性能评价及指纹图谱构建

左粟田, 余帅, 张献芳, 聂刚, 姜哲基, 彭燕, 张新全

四川农业大学草业科技学院, 四川成都 611130

通讯作者: 张新全, E-mail: zhangxq@sicau.edu.cn;彭燕, E-mail: pengyanlee@163.com
作者简介:左粟田（2001-），男，汉族，四川泸州人，硕士研究生，主要从事草种质资源创新及育种研究，E-mail：2831191073@qq.com

Abstract

Abstract: To screen new strains of Pennisetum with high yield and cold tolerance， a two-year field experiment was conducted to comprehensively evaluate the productive performance and cold tolerance of 14 newly developed Pennisetum strains， and to construct their DNA fingerprints using simple sequence repeat （SSR） molecular markers. The results showed that plant height and stem diameter of the tested materials ranged from 330.11 to 400.11 cm and 16.32 to 22.64 mm， respectively， while fresh yield and dry matter yield ranged from 162.06 to 252.47 t·hm^-2 and 23.28 to 43.06 t·hm^-2， respectively. Among all strains， GM1×FY189 and GM1×FA591 exhibited superior productive performance， with dry matter yield reaching 39.19 t·hm^-2 and 37.70 t·hm^-2， respectively. Regarding to cold tolerance， the control variety ‘Guiminyin’ （GMY） showed the earliest regreening time in the following spring， while GM1×LP176 showed the latest. The winter survival rate of GM1×FY189 was 97%， 7% higher than the control ‘Guimu No.1’ （GM1）. The winter survival rate of all tested materials exceeded 84%， with 13 strains outperforming the control， indicating that these Pennisetum strains had satisfactory overwintering ability and strong adaptability in the experimental region. Based on a comprehensive evaluation of yield and cold tolerance， two elite strains， GM1×FY189 and GM1×FA591， were selected. For molecular identification， a fingerprinting system was constructed using four previously developed core SSR primer pairs （SSR7， SSR10， SSR14， and SSR19）， which could completely distinguish all 20 tested accessions. This fingerprinting system provides a technical basis for accurate identification of elite Pennisetum germplasm and new strains.

Key words: Pennisetum, Production performance, Cold tolerance, SSR molecular markers, Fingerprint

摘要： 为筛选高产抗寒狼尾草新品系，本研究在大田条件下对创制的14份狼尾草新品系的生产性能和抗寒性进行了连续2年的综合评价，并利用简单重复序列（Simple sequence repeats，SSR）分子标记构建其指纹图谱。结果表明：供试材料的株高、茎粗范围分别为330.11~400.11 cm和16.32~22.64 mm，鲜草和干草产量范围分别为162.06~252.47 t·hm^-2和23.28~43.06 t·hm^-2。其中GM1×FY189与GM1×FA591的生产性能较优，干草产量分别达39.19 t·hm^-2和37.7 t·hm^-2。在抗寒性方面，对照品种‘桂闽引’（GMY）返青时间最早，而GM1×LP176最晚；GM1×FY189越冬率为97%，比对照品种‘桂牧1号’（GM1）高7%，供试材料越冬率均高于84%，其中13份新品系越冬率超过对照，表明供试狼尾草材料在试验区域越冬性好，具有较强的适应性。对产量与抗寒性进行综合评价，筛选出GM1×FY189和GM1×FA591两个优良新品系。分子鉴定方面，本研究利用前期开发的4对SSR核心引物（SSR7，SSR10，SSR14和SSR19）构建了供试材料的指纹图谱，该图谱可完全区分20份材料，为狼尾草优异种质及新品系的精准鉴定提供技术支撑。

关键词: 狼尾草属, 生产性能, 抗寒性, SSR分子标记, 指纹图谱

CLC Number:

S543+.9

ZUO Su-tian, YU Shuai, ZHANG Xian-fang, NIE Gang, JIANG Zhe-ji, PENG Yan, ZHANG Xin-quan. Evaluation of Production Performance and Fingerprint Construction of Pennisetum Varieties[J]. Acta Agrestia Sinica, 2026, 34(10): 3693-3703.

左粟田, 余帅, 张献芳, 聂刚, 姜哲基, 彭燕, 张新全. 狼尾草品种（系）生产性能评价及指纹图谱构建[J]. 草地学报, 2026, 34(10): 3693-3703.

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Evaluation of Production Performance and Fingerprint Construction of Pennisetum Varieties

狼尾草品种（系）生产性能评价及指纹图谱构建

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