Mycosystema

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Difference of nutritional components and metabolites in basidiomata of Auricularia cornea cultivated with three different substrates

LUO Yanglan, CHEN Lixin, WANG Canqin, WEI Jiaojun, LAN Taojü, WU Shengjin, WEI Shiyan

Mycosystema, 2024, 43(5): 230222. DOI: 10.13346/j.mycosystema.230222

试验组 Group	序号 Serial number	代谢物名称 Metabolite	变量权重值 Variable important in projection	P值 P-value	差异倍数 Fold change	物质含量变化 Change of substance content
B&A	1	山嵛酸Docosanoic acid	3.97	<0.01	2.62	↑
	2	酵母氨酸Saccharopine	1.18	<0.01	1.75	↑
	3	甘油磷酸胆碱Glycerophosphocholine	7.43	<0.01	1.64	↑
	4	亮氨酸Leucine	1.11	<0.01	1.54	↑
	5	尿苷二磷酸木糖UDP-xylose	1.63	<0.01	1.51	↑
	6	3(20)-菲烯-1,2-二醇 3(20)-Phytene-1,2-diol	3.47	<0.01	1.40	↑
	7	海藻糖-6-磷酸Trehalose-6-phosphate	1.97	<0.01	1.39	↑
	8	原儿茶醛Protocatechualdehyde	1.24	<0.01	1.38	↑
	9	Crobarbatine	1.59	<0.01	1.37	↑
	10	尿苷二磷酸-N-乙酰氨基葡萄糖 Uridine diphosphate-N-acetylglucosamine	5.71	<0.01	1.36	↑
	11	尿苷二磷酸葡糖醛酸 Uridine diphosphate glucuronic acid	1.36	<0.01	1.31	↑
	12	焦谷氨酸Pyroglutamic acid	1.52	<0.01	1.27	↑
	13	衣康酸Itaconic acid	2.91	<0.01	1.27	↑
	14	d-天冬氨酸 d-aspartic acid	1.83	<0.01	1.26	↑
	15	γ氨基丁酸 Gamma-aminobutyric acid	1.11	<0.01	1.25	↑
	16	蔗糖Sucrose	1.94	0.01	1.08	↑
	17	醋酸氯司替勃Clostebol acetate	1.79	0.02	1.05	↑
	18	甘露糖-6-磷酸Mannose 6-phosphate	1.23	<0.01	0.96	↓
	19	3-酮鞘氨醇 3-Ketosphingosine	1.00	<0.01	0.94	↓
	20	顺式13-多考酰胺 13Z-docosenamide	5.69	<0.01	0.90	↓
	21	龙胆二糖Gentiobiose	1.25	<0.01	0.88	↓
	22	溶血型磷脂酰乙醇胺LPE 18:2	1.80	<0.01	0.85	↓
	23	脑磷脂PE (18:2/0:0)	2.02	<0.01	0.81	↓
	24	芸苔素唑Brassinazole	1.05	<0.01	0.78	↓
	25	l-葡萄糖 l-glucose	1.34	<0.01	0.76	↓
	26	乳糖酸Lactobionic acid	1.99	<0.01	0.76	↓
	27	dl-阿拉伯糖 dl-arabinose	2.08	<0.01	0.74	↓
	28	脑磷脂PE (18:1/0:0)	1.34	<0.01	0.73	↓
	29	核糖内酯Ribonolactone	1.26	<0.01	0.72	↓
	30	脑磷脂PE (P-16:0/2:0)	1.68	<0.01	0.71	↓
	31	1-亚麻酰-SN-甘油-3-磷酸胆碱 1-Linoleoyl-SN-glycero-3-phosphorylcholine	3.71	<0.01	0.64	↓
	32	1-油酰基-SN-甘油-3-磷酸胆碱 1-Oleoyl-SN-glycero-3-phosphocholine	1.96	<0.01	0.63	↓
	33	Abiesadine I	1.93	<0.01	0.59	↓
	34	洋地黄甙元Digitoxigenin	1.59	<0.01	0.44	↓
	35	Chagreslactone	1.15	<0.01	0.26	↓
	36	7-[(2-O-α-L-吡喃鼠李糖基-β-D-吡喃葡萄糖基)氧基]- 4ʹ,5-二羟基-3ʹ-甲氧基黄烷酮 7-[(2-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranosyl)oxy]- 4ʹ,5-dihydroxy-3ʹ-methoxyflavanone	1.79	<0.01	0.26	↓
	37	橙皮苷Hesperidin	3.46	<0.01	0.25	↓
	38	川陈皮素Nobiletin	2.95	<0.01	0.07	↓
	39	橘皮素Tangeretin	2.77	<0.01	0.05	↓
	40	甜菜碱Betaine	2.14	<0.01	0.02	↓
	41	垂盆草苷Sarmentosin	1.61	0.04	0.94	↓
C&A	1	尿苷-5′-二磷酸钠盐Uridine 5ʹ-diphosphate	1.45	<0.01	2.17	↑
	2	川陈皮素Nobiletin	2.55	<0.01	2.07	↑
	3	左旋肉碱l-carnitine	2.69	<0.01	1.91	↑
	4	橘皮素Tangeretin	2.17	<0.01	1.86	↑
	5	海藻糖-6-磷酸Trehalose-6-phosphate	2.34	<0.01	1.82	↑
	6	山嵛酸Docosanoic acid	2.24	<0.01	1.76	↑
	7	腺苷Adenosine	1.33	<0.01	1.74	↑
	8	橙皮苷Hesperidin	2.71	<0.01	1.71	↑
	9	甘油磷酸胆碱Glycerophosphocholine	6.44	<0.01	1.71	↑
	10	γ氨基丁酸 Gamma-aminobutyric acid	1.55	<0.01	1.69	↑
	11	7-[(2-O-α-L-吡喃鼠李糖基-β-D-吡喃葡萄糖基)氧基]- 4ʹ,5-二羟基-3ʹ-甲氧基黄烷酮 7-[(2-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranosyl)oxy]- 4ʹ,5-dihydroxy-3ʹ-methoxyflavanone	1.29	<0.01	1.58	↑
	12	9-羟基-10,12-十八碳二烯酸 9-Hydroxy-10,12-octadecadienoic acid	1.03	<0.01	1.57	↑
	13	5ʹ-单磷酸尿苷Uridine 5ʹ-monophosphate	1.28	<0.01	1.55	↑
	14	Crobarbatine	1.60	<0.01	1.54	↑
	15	尿苷二磷酸木糖UDP-xylose	1.31	<0.01	1.48	↑
	16	L-谷氨酰胺L-glutamine	1.15	<0.01	1.47	↑
	17	尿苷二磷酸葡糖醛酸 Uridine diphosphate glucuronic acid	1.31	<0.01	1.41	↑
	18	单磷酸腺苷Adenosine monophosphate	1.62	<0.01	1.36	↑
	19	甘露糖-6-磷酸Mannose-6-phosphate	3.83	<0.01	1.38	↑
	20	焦谷氨酸Pyroglutamic acid	1.42	<0.01	1.34	↑
	21	尿苷二磷酸-N-乙酰氨基葡萄糖 Uridine diphosphate-N-acetylglucosamine	4.09	<0.01	1.27	↑
	22	2-巯基苯并噻唑2-Mercaptobenzothiazole	2.03	<0.01	1.25	↑
	23	d-天冬氨酸 d-aspartic acid	1.25	<0.01	1.18	↑
	24	l-谷氨酸 l-glutamic acid	1.16	<0.01	1.13	↑
	25	衣康酸Itaconic acid	1.52	<0.01	1.12	↑
	26	3(20)-菲烯-1,2-二醇 3(20)-Phytene-1,2-diol	1.11	<0.01	1.07	↑
	27	3-酮鞘氨醇3-Ketosphingosine	1.03	<0.01	0.92	↓
	28	柠檬酸Citric acid	3.86	<0.01	0.86	↓
	29	4-氧代异佛尔酮Ketoisophorone	1.20	<0.01	0.79	↓
	30	脑磷脂PE (18:1/0:0)	1.02	<0.01	0.77	↓
	31	醋酸氯司替勃Clostebol acetate	3.91	<0.01	0.75	↓
	32	脑磷脂PE (P-16:0/2:0)	1.32	<0.01	0.72	↓
	33	1-己醇1-Hexanol	1.77	<0.01	0.71	↓
	34	龙胆二糖Gentiobiose	1.74	<0.01	0.70	↓
	35	乳糖酸Lactobionic acid	1.88	<0.01	0.69	↓
	36	蔗糖Sucrose	3.62	<0.01	0.69	↓
	37	鞘氨醇Sphinganine	2.87	<0.01	0.68	↓
	38	1-庚醇1-Heptanol	5.27	<0.01	0.68	↓
	39	N,N-二甲基十二胺-N-氧化物 N,N-dimethyldodecylamine N-oxide	1.07	<0.01	0.68	↓
	40	脑磷脂PE(18:2/0:0)	2.17	<0.01	0.68	↓
	41	溶血型磷脂酰乙醇胺LPE 18:2	2.46	<0.01	0.64	↓
	42	4-p-樟烷-1,8-二醇 Cis-p-menthan-1,8-diol	1.78	<0.01	0.58	↓
	43	芸苔素唑Brassinazole	1.29	<0.01	0.54	↓
	44	Abiesadine I	1.69	<0.01	0.53	↓
	45	洋地黄甙元Digitoxigenin	1.28	<0.01	0.47	↓
	46	1-油酰基-SN-甘油-3-磷酸胆碱 1-Oleoyl-SN-glycero-3-phosphocholine	2.09	<0.01	0.38	↓
	47	1-亚麻酰-SN-甘油-3-磷酸胆碱 1-Linoleoyl-SN-glycero-3-phosphorylcholine	4.22	<0.01	0.31	↓
	48	Chagreslactone	1.04	<0.01	0.12	↓
	49	甜菜碱Betaine	1.77	<0.01	0.01	↓
B&C	1	鞘氨醇Sphinganine	2.84	<0.01	1.42	↑
	2	1-己醇1-Hexanol	1.94	<0.01	1.46	↑
	3	茉莉酮Jasmone	1.10	<0.01	1.75	↑
	4	1-庚醇1-Heptanol	5.54	<0.01	1.47	↑
	5	3(20)-菲烯-1,2-二醇 3(20)-Phytene-1,2-diol	2.69	<0.01	1.31	↑
	6	4-p-樟烷-1,8-二醇 Cis-p-menthan-1,8-diol	1.88	<0.01	1.76	↑
	7	1-亚麻酰-SN-甘油-3-磷酸胆碱 1-Linoleoyl-SN-glycero-3-phosphorylcholine	2.98	<0.01	2.02	↑
	8	醋酸氯司替勃Clostebol acetate	4.50	<0.01	1.40	↑
	9	1-油酰基-SN-甘油-3-磷酸胆碱 1-Oleoyl-SN-glycero-3-phosphocholine	1.35	<0.01	1.63	↑
	10	蔗糖Sucrose	4.24	<0.01	1.57	↑
	11	山嵛酸Docosanoic acid	2.46	<0.01	1.49	↑
	12	原儿茶醛 Protocatechualdehyde	1.31	<0.01	1.69	↑
	13	柠檬酸Citric acid	4.33	<0.01	1.18	↑
	14	4-氧代异佛尔酮Ketoisophorone	1.34	<0.01	1.27	↑
	15	龙胆二糖Gentiobiose	1.39	<0.01	1.26	↑
	16	12-O-methylsordariol	1.07	<0.01	1.21	↑
	17	溶血型磷脂酰乙醇胺 LPE 18:2	1.90	<0.01	1.32	↑
	18	衣康酸 Itaconic acid	1.79	<0.01	1.13	↑
	19	脑磷脂PE(18:2/0:0)	1.35	<0.01	1.19	↑
	20	尿苷二磷酸-N-乙酰氨基葡萄糖 Uridine diphosphate-N-acetylglucosamine	2.19	<0.01	1.07	↑
	21	海藻糖-6-磷酸 Trehalose-6-phosphate	1.72	<0.01	0.77	↑
	22	甘露糖-6-磷酸Mannose 6-phosphate	4.15	<0.01	0.70	↑
	23	橙皮素Hesperetin	1.12	<0.01	0.17	↓
	24	7-[(2-O-α-L-吡喃鼠李糖基-β-D-吡喃葡萄糖基)氧基]- 4ʹ,5-二羟基-3ʹ-甲氧基黄烷酮 7-[(2-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranosyl)oxy]- 4ʹ,5-dihydroxy-3ʹ-methoxyflavanone	2.04	<0.01	0.16	↓
	25	橘皮素 Tangeretin	3.27	<0.01	0.03	↓
	26	川陈皮素Nobiletin	3.66	<0.01	0.04	↓
	27	6-去甲氧基桔皮素6-Demethoxytangeretin	1.08	<0.01	0.03	↓
	28	左旋肉碱 l-carnitine	2.92	<0.01	0.48	↓
	29	l-葡萄糖 l-glucose	1.54	<0.01	0.65	↓
	30	5ʹ-单磷酸尿苷Uridine 5ʹ-monophosphate	1.54	<0.01	0.52	↓
	31	核糖内酯Ribonolactone	1.05	<0.01	0.73	↓
	32	尿苷-5′-二磷酸钠盐Uridine 5ʹ-diphosphate	1.28	<0.01	0.61	↓
	33	丙酰肉碱Propionylcarnitine	1.09	<0.01	0.31	↓
	34	橙皮苷Hesperidin	4.10	<0.01	0.14	↓
	35	腺苷Adenosine	1.32	<0.01	0.61	↓
	36	dl-阿拉伯糖 dl-arabinose	1.79	<0.01	0.74	↓
	37	9-羟基-10,12-十八碳二烯酸 9-Hydroxy-10,12-octadecadienoic acid	1.19	<0.01	0.55	↓
	38	单磷酸腺苷Adenosine monophosphate	1.90	<0.01	0.65	↓
	39	γ氨基丁酸 Gamma-aminobutyric acid	1.26	<0.01	0.74	↓
	40	l-谷氨酰胺 l-glutamine	1.08	<0.01	0.73	↓
	41	2-巯基苯并噻唑2-Mercaptobenzothiazole	2.12	<0.01	0.79	↓
	42	顺式13-多考酰胺 13Z-docosenamide	4.74	<0.01	0.90	↓
	43	麦角硫因Ergothioneine	1.09	<0.01	0.86	↓
	44	甘油磷酸胆碱Glycerophosphocholine	1.81	<0.01	0.96	↓

Table 2 Different metabolites among treatment groups

Other figure/table from this article

Fig. 1 The main nutritive components. Figures a-f show the nutritive components of Auricularia cornea cultivated with different substrates respectively. Different lowercase letters indicate the significant difference (P<0.05).
Fig. 2 PCA score plot. A: Miscellaneous sawdust, control; B: Eucalyptus sawdust; C: Eucalyptus bark. The same below.
Fig. 3 OPLS-DA score plot. Figures a, b and c show the OPLS-DA score plot of A&B, A&C and B&C respectively.
Table 1 Main evaluation indexes of OPLS-DA model
Fig. 4 OPLS-DA permutation test plots. Figures aʹ, bʹ and cʹ correspond to comparison groups A&B, A&C and B&C respectively. R² represents the cumulative variance value of the model; Q² represents the cumulative cross validation of the model; R²Y (cum) represents cumulative interpretation rate.
Fig. 5 Hierarchical clustering heat map. Figures a, b and c correspond to comparison groups A&B, A&C and B&C respectively. Each row represents a metabolite; Each column represents metabolites from different samples; Red indicates high metabolite content; Green indicates low metabolite content.
Table 3 KEGG pathway annotation
Fig. 6 KEGG enrichment pathway diagram. Figures a, b and c show the KEGG enrichment pathway of A&B, A&C and B&C respectively. Each bubble represents a pathway; Abscissa represents the impact value in the pathway; Ordinate represents the enrichment significance of metabolites in the pathway -log₁₀ (P-value); Bubble size represents the impact value; The larger the bubble, the greater the importance of the pathway. Bubble color from light to deep indicates that P-value decreases in turn.

模态框（Modal）标题

Figure/Table detail