Mycosystema. 2012, 31(5): 775-783.
The compounds from the EtOH-extract of Fomes fomentarius were isolated and purified by using silicagel and Sephadex LH-20 column chromatography. NMR, MS and other spectroscopy were used to identify the structures of compounds. Twelve compounds were determined: ergosta-7,22-dien-3-palmitate (1), stearic acid (2), palmitic acid (3), ergosta-7,22-dien-3-one (4), ergosta-7,22-dien-3-ol (5), 5,8-epidioxyergosta-6,22-dien-3-ol (6), ergosta-7,22-dien-3- one,dimethyl acetal (7), lup-20(29)-ene-3,28-diol,28-acetate (8), betulin (9), 3-hydroxyoctadecanoic acid (10), 9,10- dihydroxystearic acid (11), daphnetin (12). The anti-tumor activities against NCI-H 460 and SGC-7901 were evaluated by Alamar Blue method, and the results showed that compound 4 had the highest antitumor activity against NCI-H 460 and compound 9 against SGC-7901.