草地学报

草地学报 ›› 2014, Vol. 22 ›› Issue (6): 1308-1317.DOI: 10.11733/j.issn.1007-0435.2014.06.025

• 研究论文 • 上一篇    下一篇

高温胁迫对草地早熟禾抗氧化酶活性及其同工酶图谱的影响

张梅1, 刘君2, 杨志民2, 刘信宝1, 李志华1   

  1. 1. 南京农业大学动物科技学院, 江苏 南京 210095;
    2. 南京农业大学草坪研究与开发工程技术中心, 江苏 南京 210095
  • 收稿日期:2014-01-06 修回日期:2014-03-13 出版日期:2014-12-15 发布日期:2014-12-01
  • 通讯作者: 李志华
  • 作者简介:张梅(1987-),女,福建邵武人,硕士研究生,主要从事草坪生态与管理方面的研究,E-mail:zhangmei2112@163.com
  • 基金资助:

    江苏省自然基金项目(BK2009480)资助

Effects of High Temperature Stress on the Activities and Isozymes of Antioxidant Enzymes in Kentucky Bluegrass

ZHANG Mei1, LIU Jun2, YANG Zhi-min2, LIU Xin-bao1, LI Zhi-hua1   

  1. 1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China;
    2. Turf Engineering and Technology Center of Nanjing Agricultural University; Nanjing, Jiangsu Province 210095, China
  • Received:2014-01-06 Revised:2014-03-13 Online:2014-12-15 Published:2014-12-01

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摘要：

为阐明冷季型草坪草抗氧化酶对高温的胁迫响应,以草地早熟禾(Poa pratensis)耐热品种‘热力’(Thermal Blue)、中耐热品种‘大师’(Utmost)和热敏感品种‘长征’ (Excursion)为试验材料,动态研究45℃/40℃(昼/夜)高温胁迫下叶片中5种抗氧化酶活性的变化.结果表明:45℃/40℃高温胁迫使得3个草地早熟禾品种的超氧化物歧化酶(SOD)活性升高;耐热品种‘热力’ 过氧化物酶(POD)活性呈先升高后降低的趋势,‘大师’和‘长征’ POD活性变化不显著;3个草地早熟禾品种随高温胁迫时间的延长过氧化氢酶(CAT)和谷胱甘肽还原酶(GR)活性明显降低;‘热力’抗坏血酸过氧化物酶(APX)活性在胁迫第1 d和第1.5 d出现显著降低,‘大师’ APX活性胁迫第0.5 d显著降低,而第1 d显著增加,‘长征’胁迫1.5 d出现显著升高,且3个草地早熟禾品种在胁迫第2 d时APX活性均恢复到胁迫前的水平,与胁迫前相比差异不显著.同工酶表达上,3个草地早熟禾品种的SOD, POD和CAT同工酶表达量变化不显著,GR同工酶表达量减弱,APX同工酶表达量在耐热品种‘热力’中增加,在不耐热品种‘长征’中变化不大.

关键词: 草地早熟禾, 高温胁迫, 抗氧化酶, 同工酶

Abstract:

In order to understand the responses of antioxidant enzymes in cool-season turf grass under heat stress, the dynamic changes of five antioxidant enzyme activities and isozymes among different Kentucky bluegrass cultivars were investigated under 45℃/40℃(day/night) stress. The experimental cultivars were heat-tolerant ‘Thermal Blue’, heat-moderate ‘Utmost’ and heat-sensitive ‘Excursion’. The results showed that in three cultivars the activities of superoxide dismutase(SOD)were increased under 45℃/40℃(day/night) stress, whereas the activities of POD in the‘Utmost’ and ‘Excursion’ did not change significantly. The activities of peroxidase (POD) in the ‘Thermal Blue’ first increased then decreased. The activities of catalase (CAT) and glutathione reductase (GR) in the three cultivars decreased significantly with the period of high temperature stress prolonged. The activities of ascorbic acid peroxidase (APX) in the ‘Thermal Blue’ decreased significantly under heat stress for 1 d and 1.5 d, whereas the activities of APX in the ‘Utmost’ decreased under heat stress for 0.5 d then increased after 1 d, the activities of APX in the ‘Excursion’ increased significantly after 1.5 d. And the activities of APX in three tested cultivars had no significant difference after heat stress compared with before heat stress. Meanwhile, the isozymes of SOD, POD, CAT in the three cultivars remained unchanged. The expression levels of GR isozymes declined and the expression levels of APX isozymes increased in heat-tolerant cultivar ‘Thermal Blue’, whereas the expression levels of APX isozymes did not change in heat-sensitive cultivar ‘Excursion’.

Key words: Kentucky bluegrass, High temperature stress, Antioxidant enzyme, Isozyme

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