苜蓿根系酯酶及内源激素变化与根蘖发生的研究

doi:10.11733/j.issn.1007-0435.1994.02.001

›› 1994, Vol. 2 ›› Issue (2): 1-11.DOI: 10.11733/j.issn.1007-0435.1994.02.001

• 研究论文 • 下一篇

苜蓿根系酯酶及内源激素变化与根蘖发生的研究

高振生, 王培, 洪绂曾, 谢雪菊

北京农业大学动物科技学院草地所, 北京100094

出版日期:1994-08-15 发布日期:2012-07-11
基金资助:
高等学校博士点基金

Change of Esterase Isozyme and Endogenous Hormone in Root System and Ontogenesis of Creeping-Rooted Character in Alfalfa

Gao Zhensheng, Wang Pei, Hong Fuzeng, Xie Xueju

Institute of Grassland Science, Beijing Agricultural University Beijing 100094, China

Online:1994-08-15 Published:2012-07-11

摘要/Abstract

摘要： 在植物生长发育的不同阶段(5/16、6/25、8/9、9/21)测定了根蘖苜蓿和非根蘖苜蓿植株根组织中的酯酶同工酶及根系不同组织部位中五种内源激素(Z、ZR、IAA、GA₃和ABA)含量,并比较、分析根蘖植株与非根蘖植株酯酶和内源激素动态及根蘖植株根蘖芽的形成和生长与激素的关系。研究结果表明,根蘖与非根蘖苜蓿植株根组织酯酶同工酶带数明显不同,根蘖苜蓿植株明显高于非根蘖苜蓿植株2～4条谱带,这说明苜蓿根组织酯酶同工酶可能与苜蓿根蘖性状的基因表达有关。根系中内源激素分析结果表明,根蘖与非根蘖苜蓿植株根颈和根组织中(Z+ZR)、IAA、GA₃含量均在植物生长发育旺盛时期(6/25、8/9)最高。ABA含量随植株生长发育而呈上升趋势。根蘖植株的(Z+ZR)、IAA、GA₃和ABA含量在整个生育期中的变化趋势与非极蘖植株不同,而来源于根蘖型品系中非根蘖植株与当地品种主根植株的变化规律相似。根蘖苜蓿植株根系中IAA/(Z+ZR)低于非根蘖植株。苜蓿植株根系中内源激素(Z+ZR)、IAA、GA₃含量与苜蓿根蘖芽形成和生长有关。根蘖植株根系内不同组织部位内源激素分布不一致,根蘖苜蓿植株水平根膨大与内源激素GA₃含量显著增加有关。

关键词: 根蘖苜蓿, 内源激素, 酯酶同工酶

Abstract: The esterase isozyme in the root tissues and the contents of endogenous hormones such as Z, ZR, IAA, GA, and ABA, in the different parts of root system for the creeping-roofed and noncreepingrooted alfalfa plants were measured at various growing stages (May 16th, June 25th, August 9th and Sept.21th).The dynamics of esterase isozyme and endogenous hormones in the creeping-rooted and noncreeping rooted alfalfa plants and the relationship between the formation and growth of adventitious bud and the hormone contents were compared and analysed.It is showed that the esterase isozyme bands in the root system of creeping-rooted and noncreepingrooted alfalfa are obviously different, the band numbers of esterase isozyme in the creeping-rooted alfalfa plant is significantly higher than those in the noncreeping-rooted alfalfa plant.It means that the esterase isozyme in the alfalfa root system is consistent with the gene expression of its creeping-rooted character.The analysis results also showed that the peak contents of Z+ZR, GA₃ and IAA in the crown and root tissues of creeping-rooted and noncreeping-rooted alfalfa plants are at the vigorous growing stage (June 25th and August 9th).The ABA content increases along with the continuous growth and development.The seasonal change of Z+ZR, IAA and GA, in the creeping-rooted plant is different from that in the noncreeping-rooted plant, while those in the noncreeping-rooted plants of two different genotypes are similar.The ratio of IAA/(Z+ZR), or (IAA + GA₃)/(Z + ZR) in the root system of creeping-rooted plant are lower than that of noncreeping-rooted plant.It is concluded that the initiation and growth of stem primordia is associated with the contents of Z+ZR, IAA and GA₃.The distribution of endogenous hormones in the different root tissues of creeping-rooted alfalfa is uneven.The formation of expansion in horizontal root of creeping-rooted alfalfa is related with the significant increase of ondogenous GA₃.

Key words: Creeping-rooted alfalfa, Endogenous hormone, Esterase isozyme

高振生, 王培, 洪绂曾, 谢雪菊. 苜蓿根系酯酶及内源激素变化与根蘖发生的研究[J]. , 1994, 2(2): 1-11.

Gao Zhensheng, Wang Pei, Hong Fuzeng, Xie Xueju. Change of Esterase Isozyme and Endogenous Hormone in Root System and Ontogenesis of Creeping-Rooted Character in Alfalfa[J]. , 1994, 2(2): 1-11.

参考文献

[1] 洪级曾、吴义顺、白水和、高振生、樊奋成、程渡,1991,苜蓿根蘖性状的研究,中国草地,1:27-30
[2] 胡能书、万贤国编,1985,《同工酶技术及其应用》,湖南科学技术出版社,96~104
[3] 刘杰、曹亦芬、符义坤、曹致中,1992,应用同工酶分析及研究不同首楷品种的生态学特性,甘肃农业大学学报,2:138~143
[4] 马兴林、梁振兴,1993,小麦分蘖发生、衰亡过程中内源激素的研究,《第一届全国青年作物栽培、作物生理学术会论文集》,中国科学技术出版社,32~35
[5] 张明先、郝润鲜,1993,苜蓿品种生态型及其演变的同工酶电泳数据分析,中国草地,3:49~55
[6] 张辉、陈鸿山、王宜林、罗忠旺、丁维,1991,核不育亚麻不育株与可育株同工酶分析,作物学报,Vol. 17,3:198~203
[7] 周时佳、韩敬花,1985,太谷核不育小麦可育株、不育株及T型不育系小麦同工酶的比较研究,作物学报,Vol. 11:109~114
[8] 周瑞莲、张承烈,1991, PEG渗透胁迫对不同抗逆性紫花首蓓品种过氧化物酶和过氧化氢酶同工酶的影响,中国草地,5:21~25
[9] 王承斌、赵来喜、海淑珍、王雪丽,1989,牧草过氧化物酶同工酶与抗寒性的初步研究,中国草地,5:72~74
[10] 李聪、毛培胜、杨青川,1992, Report of the thirty-third North American alfalfa improvement conference:p47
[11] Becana,M.,P. Aparicio-tejo.,J. J. Irigoyen,and M. Sanchez-diaz, 1986,Plant plysiology 82 (4):1169~1171
[12] Chen, T. H. H. and F. S. C. Chen, 1988, Can. J. Bot. Vol. 66:2491~2498
[13] Cowett,E. R. and M. A. Sprague,L962,Agron. J. 54:294~297
[14] Daday,H.,A. Grassia,and J. Peak,1974,Aust. J. Exp. Agric. Anim. Husb,14:735~741
[15] Davies,F. T. Jr. and B. C. Moser.,1980,J. Amen Soc. Hort. Sic,105:27~30
[16] Davies,P. J.,1987,Plant hormones and their role in plant growth and development. Martin Nijhoff Publ. New York.,NY
[17] Gramshaw, D.,H. G. Bishop,and D. H. Ludke.,1982,Aust. J. Exp. Agric. Anim. Husb,22:177~181
[18] Heinrichs,D. H.,1954,Can. J. Agr. Sci,34:269~280
[19] Heinrichs,D. H, 1958, Can. Dept. Agr. Publ 1030:5~15
[20] Heinrichs,D. H.,1963,Adv. Gron,15:317~337
[21] Irigoyen, J. J.,D. W. Emerich,and M. Sanchez-diaz.,1992,Physiol. Plant,84(1):67~72
[22] Kilcher,M. R. and D. H. Heinrichs.,1969,Can. J. Plant Sic,Vol. 49:307~311
[23] Mackay, J. H.,1977,J. Austr. Insti. Agri. Sci,43. 182~183
[24] Massengale,M. A. and J. T. Meddler.,1958, Agron. J,50: 377~380
[25] Murray,B. E.,1957,Can. J. Botany, 35:463~475
[26] Rikin,A. M.,Waldman,A. E. Richmond, and A. Dovrat.,1975,J. Exp. Bot,Vol. 26:175~183
[27] Sharif , R. and J. E. Dale.,1980.,J. Exp. Rot.,31:921~930
[28] Tomkins,J. P. and M. Hall.,1991.,Published in Agron. J 83:577~581
[29] Vance,C. P. 1978. Phytochemistry. 17(11) 1889~1891
[30] Waldman,M,A. Rikin,A. Dovrat,and A. E. Richmond,1975,J. Exp. Bot,26:353~859

苜蓿根系酯酶及内源激素变化与根蘖发生的研究

Change of Esterase Isozyme and Endogenous Hormone in Root System and Ontogenesis of Creeping-Rooted Character in Alfalfa

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