草地学报

›› 2007, Vol. 15 ›› Issue (1): 7-12.DOI: 10.11733/j.issn.1007-0435.2007.01.002

• 研究论文 • 上一篇    下一篇

超薄层等电聚焦电泳技术检验燕麦种子的真实性

刘敏轩1, 韩建国1, 王赟文1, 周青平2, 乔安海3   

  1. 1. 中国农业大学草地研究所, 农业部牧草与草坪草种子质检中心(北京), 北京, 100094;
    2. 青海牧科院草原研究所, 西宁, 810003;
    3. 青海省草原工作站, 西宁, 810008
  • 收稿日期:2006-02-21 修回日期:2006-08-28 出版日期:2007-02-15 发布日期:2007-02-15
  • 通讯作者: 王贇文,E-mail:wyw @cau.edu.cn
  • 作者简介:刘敏轩(1981- ),女,内蒙乌盟人,博士研究生,研究方向为牧草种子科学
  • 基金资助:
    “十五”国家攻关子课题(2004BA528B-2-4)

Varietal Identification of Oat(Avena spp.)by Ultrathin-layer Isoelectric Focusing of Seed Protein

LIU Min-xuan1, HAN Jian-guo1, WANG Yun-wen11, ZHOU Qing-ping2, QIAO An-hai3   

  1. 1. Institute of Grassland Science, China Agricultural University, Beijing 100094, China;
    2. Institute of grassland Science, Qinghai University, Xining, Qinghai Province 810003, China;
    3. Grassland Service Station of Qinghai Province, Xining, Qinghai Province 810008, China
  • Received:2006-02-21 Revised:2006-08-28 Online:2007-02-15 Published:2007-02-15

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摘要： 利用种子醇溶蛋白超薄层等电聚焦电泳(UTL IEF)技术对青海和河北省燕麦属(Avena spp)16个品种真实性进行了比较分析。结果表明,参试样品的种子醇溶蛋白UTL IEF电泳图谱共分离出34条不同迁移率的谱带;依据蛋白电泳图谱等电点(P I值)为4.8、6.55、6.9、7.7、8.4和8.6等位置谱带的有无以及数目,可鉴别16个参试样品中的13个样品;对同一样品的整粒种子、1/2粒和1/4粒种子,以及人工老化处理获得的同一样品不同发芽率的种子进行超薄层等电聚焦电泳,结果表明破损程度到1/2粒种子和不同生活力种子甚至死种子对电泳鉴定结果基本没有影响。

关键词: 燕麦, 超薄层等电聚焦电泳, 种子贮藏蛋白, 品种真实性鉴定

Abstract: Seed prolamine variation among sixteen oat(Avena spp.)seed samples,consisting of nine varieties and four samples of unknown names cultivated in Qinghai Province,and three varieties cultivated in Hebei Province,was analyzed using ultrathin-layer isoelectric focusing electrophoresis(UTLIEF).The results show that among the tested varieties,a total of 34 bands were identified.Based on distinctive bands at PI point of 4.8,6.55,6.9,7.7,8.4,and 8.6,respectively.Thirteen of the 16 tested samples differentiated from each other.In the present paper,the whole seed,1/2 seed units and 1/4 seed units of Qingyongjiu No.473,Canadian oat,Bayou No.5,and U4,as well as the seeds of Canada oat after aging treatment were electrophoresed by UTLIEF.The results prove that seed variability and damaged seeds of half of their original size,or even dead seeds did not affect the electrophoresis results.

Key words: Oat, Ultrathin-layer isoelectric focusing, Seed storage protein, Varieties identification

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