禾谷缢管蚜与内生真菌互作对醉马草幼苗的生理影响

doi:10.11733/j.issn.1007-0435.2008.03.006

›› 2008, Vol. 16 ›› Issue (3): 239-244,261.DOI: 10.11733/j.issn.1007-0435.2008.03.006

禾谷缢管蚜与内生真菌互作对醉马草幼苗的生理影响

张兴旭, 陈娜, 李春杰, 南志标

农业部草地农业生态系统学重点开放实验室, 兰州大学草地农业科技学院, 甘肃草原生态研究所, 甘肃, 兰州, 730020

收稿日期:2007-08-06 修回日期:2007-11-09 出版日期:2008-06-15 发布日期:2008-06-15
作者简介:张兴旭(1983- ),男,甘肃临泽人,硕士研究生,研究方向为禾草-内生真菌共生体
基金资助:
国家重点基础研究发展计划(973项目)课题(2007CB108902);国家自然科学基金项目(30771531);甘肃省中青年科技基金项目(3ZS041-A25-003)资助

Physiological Effects of Rhopalosiphum padi on Achnatherum inebrians Keng Infected by Endophyte

ZHANG Xing-xu, CHEN Na, LI Chun-jie, NAN Zhi-biao

Key Laboratory of Grassland Argo-Ecosystem, Ministry of Agriculture;College of Pastoral Agriculture Science and Technology, Lanzhou University;Gansu Grassland Ecological Research Institute;Lanzhou, Gansu Province 730020, China

Received:2007-08-06 Revised:2007-11-09 Online:2008-06-15 Published:2008-06-15

摘要/Abstract

摘要： 通过测定一系列生理变化指标,研究人工接种条件下禾谷缢管蚜(Rhopalosiphumpadi (L.))对带内生真菌(E+)与不带菌(E-)醉马草(Achnatherum inebrians (Hance) Keng)幼苗的影响.结果表明:在一定时期内,禾谷缢管蚜刺吸可使醉马草幼苗叶片相对含水量和叶绿素含量显著降低(P<0.05),同一个虫口密度E+与E-植株之间差异显著(P<0.05);幼苗体内SOD活性和POD活性均随危害时间的延长而显著升高(P<0.05),同一个虫口密度E+与E-植株之间差异显著(P<0.05);接种蚜虫可使MDA和游离脯氨酸的含量显著提高(P<0.05),同一个虫口密度内E+与E-植株之间差异显著(P<0.05).综上所述,内生真菌的侵染可以在一定程度上间接影响禾谷缢管蚜取食,进而影响醉马草幼苗生理生化的变化.

关键词: 醉马草, 禾谷缢管蚜, 内生真菌, 叶绿素, 保护酶, 脯氨酸

Abstract: The physiological indices,e.g.leaf relative water content(LRWC),chlorophyll content(CC),the activities of SOD and POD,and the contents of MDA and praline,of the endophyte-infected(E+) and endophyte-free(E-) drunken horse grass(Achnatherum inebrians(Hance) Keng) seedlings inoculated by oat-bird cherry aphid(Rhopalosiphum padi(L.)) were measured under different pest densities and different feeding periods.The results show that LRWC and CC were decreased significantly(P<0.05) by the aphid feeding in comparison with the control,the difference between E+ and E-were significant(P<0.05).The activities of SOD and POD were increased significantly(P<0.05) with the feeding periods and aphid densities,respectively,and significant(P<0.05) difference existed between E+ and E-only under 20 aphids/plant for 4 days.The contents of MDA and proline were increased significantly(P<0.05) by the inoculation of aphid for a given period and the difference between E+ and E-were significant(P<0.05).In summary,the grass endophyte could affect the feeding behavior of R.padi to a certain extent and further the physiological responses of the drunken horse grass.

Key words: Drunken horse grass, Oat-bird cherry aphid, Neotyphodium endophyte, Chlorophyll, Protective enzyme, Proline

中图分类号:

张兴旭, 陈娜, 李春杰, 南志标. 禾谷缢管蚜与内生真菌互作对醉马草幼苗的生理影响[J]. , 2008, 16(3): 239-244,261.

ZHANG Xing-xu, CHEN Na, LI Chun-jie, NAN Zhi-biao . Physiological Effects of Rhopalosiphum padi on Achnatherum inebrians Keng Infected by Endophyte[J]. , 2008, 16(3): 239-244,261.

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参考文献

[1] 史志诚.中国草地重要有毒植物[M].北京:中国农业出版社,1997.166-176
[2] Siegel M R,Latch G C M,Johnson M C.Fungal endophytes of grasses[J].Ann.Rev.Phytopathol.,1987,25:293-315
[3] Nan Z B,Li C J.Neotyphodium in native grasses in China and observations on endophyte/host interaction[A].In:Paul V H,Dapprich P D,eds.Proceedings of the 4th International Neotyphodium/Grass Interactions Symposium[C].Soest.Germany,2000.41-50
[4] Li C J,Nan Z B,Paul V H,et al.A new Neotyphodium species symbiotic with drunken horse grass (Achnatherum inebrians) in China[J].Mycotaxon,2004,90(1):141-147
[5] Li C J,Nan Z B,Li F.Biological and physiological characteristics of Neotyphodium gansuense symbiotic with Achnatherum inebrians[J].Microbiological Research,2006,(doi:10.1016/j.micres.2006.07.007)
[6] 李飞.内生真菌对醉马草抗旱性影响的研究[D] :[硕士学位论文).兰州:兰州大学,2007
[7] 缑小嫒.内生真菌对醉马草耐盐性影响的研究[D] :[硕士学位论文].兰州:兰州大学,2007
[8] Li C J,Gao J,Nan Z B.Interactions of Neotyphodium gansuense,Achnatherum inebrians and plant-pathogenic fungi.Mycological Research,2007,(doi:10.1016/j.mycres.2007.08.012)
[9] Li C J,Zhang X,Li F,et al.Disease and pests resistance of endophyte infected and non-infected drunken horse grass[A].In:Popay A,Thom ER,eds.Proceedings of the 6th International Symposium on Fungal Endophytes of Grasses[C].Dunedin,New Zealand:New Zealand Grassland Association.2007.111-114
[10] Popay A J,Bonos S A.Biotic responses in endophytic grasses[A].In:Roberts C A,West C P and Spiers D E ed.Neotyphodium in Cool-season Grasses,2005,163-185
[11] Malinowski D P,Belesky D P,Lewis G C.Abiotic stresses in endophyte grasses[A].In:Roberts C A,West C P,Spiers D E,eds.Neotyphodium in Cool-season Grasses[C].2005.187-199
[12] 南志标,李春杰.禾草内生真菌共生体在草地农业系统中的作用[J].生态学报,2004,24(3):605-616
[13] Brem D,Leuchtmann A.Intraspecific competition of endophyte infected vs.uninfected plants of two woodland grass species[J].Oikos,2002,96(2):281-290
[14] Mohammad R S,Bijan H,Aghafakhr M.Mealybug,Phenococcus solani,and barley aphid,Sipha maydis,response to endophyte-infected tall fescueand meadow fescues[J].Entomologia Experimentaliset Applicata,2004,113:205-209
[15] 娄永根,程家安.植物的诱导抗虫性[J].昆虫学报,1997,40(3):320-331
[16] Bristow C M.Host development offers new insight in to insect-plant interaction[J].Trends Ecol.Evol.,1989,5(4):123-124
[17] 杨广,徐清元,尤民生.小菜蛾取食上海青的保护酶活力变化[J].华东昆虫学报,2004,13(1):48-54
[18] Woodhead S,Bernays E.Changes in release rates of cyanide in relation to palatability Of sorghum to insects[J].Nature,1997,270:235-236
[19] 王海波,陶芸,金沙.蚕豆叶片几丁质酶活性的蚜虫诱导-植物生理应激反应的趋同性[J].应用生态学报,1994,5(1):68-71
[20] 南志标.内生真菌在我国部分国产和引进禾草品种的幼苗和成株的分布[J].草业学报,1996,5(3):13-18
[21] Hunt M G,Newman J A.Reduced herbivore resistance from a novel grass-endophyte association[J].Journal of Applied Ecology,2005,42:762-769
[22] 李合生.植物生理生化实验原理与技术[M].北京:高等教育出版社,2000:134-278
[23] Zhang Y P,Nan Z B.Growth and anti-oxidative systems changes in Elymus dahuricus is affected by Neotyphodium en-dophyte under contrasting water availability[J].Journal Ag-ronomy & Crop Science,2007,193:377-386
[24] 陈世苹,高玉葆,梁宇,等.水分胁迫下内生真菌感染对黑麦草叶内游离脯氨酸和脱落酸含量的影响[J].生态学报,2001,21(12):1964-1972
[25] Wang Z L,Huang B R.Physiology recovery of kentucky blue-grass from simultaneous drought and heat stress[J].Crop Science,2004,44:1729-1736

禾谷缢管蚜与内生真菌互作对醉马草幼苗的生理影响

Physiological Effects of Rhopalosiphum padi on Achnatherum inebrians Keng Infected by Endophyte

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