草地早熟禾转CMO-BADH双基因和转CMO基因耐盐性分析

doi:10.11733/j.issn.1007-0435.2008.04.007

›› 2008, Vol. 16 ›› Issue (4): 353-358.DOI: 10.11733/j.issn.1007-0435.2008.04.007

草地早熟禾转CMO-BADH双基因和转CMO基因耐盐性分析

徐冰¹, 韩烈保^1,2, 姚娜¹, 王瑶瑶¹, 程晓霞¹, 曾会明¹

1. 北京林业大学草坪研究所, 北京, 100083;
2. 长江大学园艺园林学院, 湖北省荆州市, 430025

收稿日期:2007-09-27 修回日期:2007-11-20 出版日期:2008-08-15 发布日期:2008-08-15
通讯作者: 韩烈保,E-mail:hanlb@tom.com
作者简介:徐冰(1982- ),男,硕士研究生,研究方向为草地早熟禾转基因育种,E-mail:foye866@163.com
基金资助:
国家863计划(2006AA10Z132);国家植物转基因产业化专项(J2002-B-006)

Salt Tolerance Analysis of Transgenic Kentucky Bluegrass with CMO-BADH Double Gene and CMO Gene

XU Bing¹, HAN Lie-bao^1,2, YAO Na¹, WANG Yao-yao¹, CHENG Xiao-xia¹, ZENG Hui-ming¹

1. Institute of Turfgrass Science, Beijing Forestry University, Beijing 100083, China;
2. College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei Province 430025, China

Received:2007-09-27 Revised:2007-11-20 Online:2008-08-15 Published:2008-08-15

摘要/Abstract

摘要： 通过对非转基因草地早熟禾(Poa pratensis L.)、转CMO-BADH双基因草地早熟禾、转CMO基因草地早熟禾进行不同浓度的NaCl胁迫试验,测定其细胞膜的透性,丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、氧化氢酶(CAT)活性,评定各株系耐盐能力的强弱,同时还验证并比较了CMO-BADH双基因和CMO基因的耐盐性功能,为耐盐新品种的选育提供了理论依据。结果表明:所有株系的相对电导率、MDA含量均随盐浓度增加而增大,在NaCl胁迫下相对电导率和MDA含量的大小顺序为:非转基因株系>转CMO基因株系>转CMO-BADH双基因株系;在NaCl的胁迫下,各个株系的SOD活性、POD活性、CAT活性都有明显的提高,此3种指标的大小顺序为:转CMO-BADH双基因株系>转CMO基因株系>非转基因株系;综合考虑各个指标,各株系耐盐性强弱顺序为:转CMO-BADH双基因株系>转CMO基因株系>非转基因株系。

关键词: CMO-BADH双基因, CMO基因, 草地早熟禾, 耐盐性

Abstract: Kentucky bluegrass is one of the main cool-season turfgrasses in North of China and the market demand is huge.The seeds of Kentucky bluegrass used in China are all from the overseas so far and their adaptability is poor in view of plant diseases and pest damages.Therefore,the breeding of Kentucky bluegrass is very important in China.In order to test and compare the salt tolerance of all lines and the functions of CMO-BADH double gene and CMO gene and provide theoretical evidence for salt-tolerant new variety breeding,the effect of NaCl stresses was studied in this paper on the tissue culture of transgenic Kentucky bluegrass lines with CMO-BADH double gene and CMO gene.The relative electrical conductivity,MDA content,SOD activity,CAT activity,and POD activity were measured.The results show that the relative electrical conductivity and MDA content of all lines were increased along with increased salt stress and the order of all lines in those two physiological traits was CK>transgenic Kentucky bluegrass with CMO gene>transgenic Kentucky bluegrass with CMO-BADH double gene.The SOD activity,CAT activity,and POD activity of all lines were also increased under the salt stress and the order of all lines in those three physiological traits was transgenic Kentucky bluegrass with CMO-BADH double gene>transgenic Kentucky bluegrass with CMO Gene>CK.In conclusion,the CMO gene and CMO-BADH double genecould both enhance the salt tolerance of transgenic Kentucky bluegrass,but the CMO-BADH double gene performed better than CMO gene. In summary of all the physiological traits,the salt tolerance order of all tested lines was transgenic Kentucky bluegrass with CMO-BADH double gene>transgenic Kentucky bluegrass with CMO gene>CK.

Key words: CMO-BADH double gene, CMO gene, Kentucky bluegrass, Salt tolerance

中图分类号:

Q943

徐冰, 韩烈保, 姚娜, 王瑶瑶, 程晓霞, 曾会明. 草地早熟禾转CMO-BADH双基因和转CMO基因耐盐性分析[J]. , 2008, 16(4): 353-358.

XU Bing, HAN Lie-bao, YAO Na, WANG Yao-yao, CHENG Xiao-xia, ZENG Hui-ming. Salt Tolerance Analysis of Transgenic Kentucky Bluegrass with CMO-BADH Double Gene and CMO Gene[J]. , 2008, 16(4): 353-358.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2008.04.007

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2008/V16/I4/353

参考文献

[1] 赵桂琴.早熟禾育种的研究进展与现状[J].甘肃农业大学学报,2000,35(6):119-126
[2] 张丽霞,姚爱兴.国内外草地早熟禾育种技术及其研究进展[J].宁夏农学院学报,2002,23(2):76-79
[3] 江香梅,黄敏仁,王明庥.植物甜菜碱合成途径及基因工程研究进展[J].中国生物工程杂志,2002,22(4):49-56
[4] Rathinasabapathi B,Burnet M,Russell B L,et al.Choline monooxygenease,an unusual iron sulfur enzyme catalyzing the first step of glycine betaine synthesis in plants:prosthetic group characterization and cDNA cloning[J].Proc.Natl.Acad.Sci.,1997,94:3454-3458
[5] Weretilnky E A,Hanson A D.Molecular cloning of a plant betaine-aldehyde dehydrogenase and enzyme implicated in adaptation to Salinity and drought[J].Proc.Natl.Acad.Sci.,1990,87:2745-2749
[6] Zhang G.Enhancing salt and drought tolerance of Poa and triploid Cynodon with BADH gene and somaclonal variation[D] :[Dissertation].New Brunswick,NJ:Rutgers University,2003
[7] 申晓辉.菠菜胆碱单氧化酶(CMO)基因克隆表达及对北美乔松转化的研究[D].北京:北京林业大学,2002
[8] 信金娜,韩烈保,刘君,等.基因枪转化法获得草地早熟禾(Poa pratensis L. )转基因植株[J] ,中国生物工程杂志,2006,26(8):10-14
[9] 何宇锋,刘君,韩烈保,等.CMO,BADH双基因植物表达载体的构建[J] ,生物技术通报,2005,05:63-66
[10] 中国科学院上海植物生理研究所,上海市植物生理学会.现代植物生理学实验指南[M].北京:科学出版社,1999.302-303
[11] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.260-261
[12] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.167-169
[13] 高俊凤.植物生理学实验技术[M].西安:世界图书出版社公司,2000.194-195
[14] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.164-165
[15] 李艳霞,姜静,于影,等.转柽柳eIF1A基因烟草的耐盐性分析[J].生物技术通讯,2006,17(03):328-331
[16] 姜静,于影,赵鑫,等.转LEA基因烟草的耐盐性分析[J].生物技术,2006,16(01):16-20
[17] 白爽,宋启平,刘桂丰,等.转betA基因的小黑杨花粉植株耐盐性分析[J].分子植物育种,2006,4(01):41-44
[18] 佘建明,张保龙,何晓兰,等.草地早熟禾农杆菌介导法基因转化条件[J].草地学报,2005,(13)1:39-42

草地早熟禾转CMO-BADH双基因和转CMO基因耐盐性分析

Salt Tolerance Analysis of Transgenic Kentucky Bluegrass with CMO-BADH Double Gene and CMO Gene

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	左郎, 王树森, 马迎梅, 温苏雅勒图, 白刚, 郁鑫明. 火炬树浸提液对两种草坪草种子萌发的影响[J]. 草地学报, 2021, 29(9): 1927-1933.
[2]	金一锋, 陈阳, 齐欣, 高岩松, 金忠民, 赵清峰, 王琦. 草地早熟禾NRT1/PTR FAMILY 8.3基因的克隆及表达分析[J]. 草地学报, 2021, 29(7): 1397-1405.
[3]	朱琨, 郑桂华, 刘丽杰, 周璐, 李珊珊, 范震宇, 钟玥, 尼尼. 草地早熟禾叶片表皮解剖结构与抗白粉病性的研究[J]. 草地学报, 2021, 29(7): 1430-1435.
[4]	张韦钰, 王春勇, 杜红梅. 富氢水对草地早熟禾耐盐性的影响以及与抗氧化酶活性的关系[J]. 草地学报, 2021, 29(7): 1436-1445.
[5]	朱琨, 刘丽杰, 周璐, 李珊珊, 范震宇, 吴纪一, 尼尼. 外源激素对染白粉菌草地早熟禾叶片膜透性及表面结构的影响[J]. 草地学报, 2021, 29(6): 1210-1216.
[6]	朱瑞婷, 牛奎举, 张娣君, 杨阳, 宋云, 马晖玲. 外源2,4-表油菜素内酯对镉胁迫下草地早熟禾叶绿素代谢的影响[J]. 草地学报, 2021, 29(4): 635-643.
[7]	高娅楠, 韩烈保, 许立新. 乙烯利对干旱胁迫下草地早熟禾抗氧化酶基因表达的影响[J]. 草地学报, 2021, 29(10): 2200-2213.
[8]	张然, 马祥, 朱瑞婷, 牛奎举, 赵春旭, 马晖玲. 青海野生草地早熟禾响应干旱胁迫的代谢通路及转录调控分析[J]. 草地学报, 2020, 28(6): 1508-1518.
[9]	赵春旭, 张然, 牛奎举, 朱瑞婷, 王勇, 马祥, 马晖玲. 青海野生草地早熟禾响应低温胁迫的代谢组学研究[J]. 草地学报, 2020, 28(4): 904-914.
[10]	周彤, 师尚礼, 陈建纲, 吴芳, 宫文龙, 来幸樑, 阿芸, 张成君. 紫花苜蓿与草地早熟禾轮作模式对其农艺性状和营养品质的影响[J]. 草地学报, 2020, 28(3): 720-726.
[11]	赵春旭, 马祥, 董文科, 牛奎举, 张然, 马晖玲. 低温胁迫下不同青海野生草地早熟禾的转录组比较分析[J]. 草地学报, 2020, 28(2): 305-318.
[12]	李雪, 沙栢平, 高雪芹, 王焱, 伏兵哲. 不同紫花苜蓿种质材料萌发期耐盐性鉴定与综合评价[J]. 草地学报, 2020, 28(2): 437-445.
[13]	张则宇, 李雪, 王焱, 常巍, 高雪芹, 伏兵哲. 59份苜蓿种质材料苗期耐盐性评价及耐盐指标筛选[J]. 草地学报, 2020, 28(1): 112-121.
[14]	张金青, 陈金龙, 李凡, 赵炳森, 贾新凤, 马晖玲. 草地早熟禾种子萌发和幼苗生长对铅胁迫的适应性[J]. 草地学报, 2020, 28(1): 130-140.
[15]	鲜靖苹, 王勇, 马晖玲. 外源NO缓解草地早熟禾镉胁迫的转录组分析[J]. 草地学报, 2019, 27(6): 1467-1476.