草地学报

›› 2012, Vol. 20 ›› Issue (5): 907-913.DOI: 10.11733/j.issn.1007-0435.2012.05.018

• 研究论文 • 上一篇    下一篇

NaCl胁迫对唐古特白刺幼苗生理指标的影响

王文1,2, 蒋文兰1,2, 谢忠奎3, 张德罡1,2, 寇江涛1,2   

  1. 1. 甘肃农业大学草业学院, 甘肃 兰州 730070;
    2. 草业生态系统教育部重点实验室 甘肃省草业工程实验室 中-美草地畜牧业可持续研究中心, 甘肃 兰州 730070;
    3. 中国科学院寒区旱区环境与工程研究所, 甘肃 兰州 730000
  • 收稿日期:2012-04-18 修回日期:2012-06-25 出版日期:2012-10-15 发布日期:2012-11-01
  • 通讯作者: 蒋文兰,E-mail:jwl66666@sina.com
  • 作者简介:王文(1964-),男,甘肃庆阳人,博士研究生,研究方向为草地生态、资源与环境,E-mail:gswwen@126.com
  • 基金资助:
    国家自然科学基金"地表覆盖层结构特征的参数及与水文过程的关系研究"(41171027);国家牧草产业技术体系岗位科学家基金(CARS-35)资助

Effect of NaCl Stress on Physiological Index of Nitraria tangutorum Seedling

WANG Wen1,2, JIANG Wen-lan1,2, XIE Zhong-kui3, ZHANG De-gang1,2, KOU Jiang-tao1,2   

  1. 1. College of Pratacultural Science, Gansu Agricaltural University, Lanzhou, Gansu Province 730070, China;
    2. Key Laboratory of Ecosystem Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-US Center for Grazingland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China;
    3. Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, Gansu Province 730000, China
  • Received:2012-04-18 Revised:2012-06-25 Online:2012-10-15 Published:2012-11-01

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摘要： 为明确唐古特白刺(Nitraria tangutorum Bobr.)对NaCl胁迫的生理响应特征,采用盆栽法研究不同NaCl浓度(25,50,75,100,200,300,400 mmol·L-1,以0 mmol·L-1为对照)对其幼苗叶片抗氧化物酶活性及渗透调节物质含量的影响。结果表明:胁迫10,20,30 d后,抗氧化物酶活性随胁迫浓度的增大呈相似的规律性变化,超氧化物歧化酶(SOD)、过氧化氢酶(CAT) 和过氧化物酶(POD)活性均在低浓度盐胁迫(25~100 mmol·L-1)时上升,高浓度盐胁迫(200~400 mmol·L-1)时下降;丙二醛(MDA)含量随盐浓度增加持续上升;脯氨酸(Pro)含量在胁迫10,20,30 d后,均随盐浓度增高而持续上升,同一盐浓度下,10~20 d上升幅度较大、20~30 d上升幅度较小;可溶性蛋白质(SP)含量呈整体下降趋势,可溶性糖(SS)含量在低盐浓度胁迫时上升,高盐浓度胁迫时下降。

关键词: NaCl胁迫, 唐古特白刺幼苗, 抗氧化酶, 渗透调节物质

Abstract: The response of antioxidase and osmoregulation materials in leaf of Nitraria tangutorum seedling to NaCl stress with different concentration (25,50,75,100,200,300,400 mmol稬-1) for 10, 20 and 30 days was studied with pot experiment. The result indicated that the activity of antioxidase increased along with the stress concentration. The activity of superoxide dismutase (SOD), catalases (CAT) and peroxidase (POD) increased under the low concentration stress (25 to 100 mmol稬-1) and decreased under high concentration (200 to 400 mmol稬-1). The content of Malonate (MDA) kept increasing under stress, The proline (Pro) content increased along with the stress concentration. And the increasing amount was higher within 10 to 20 days compared to that within 20 to 30 days under same stress concentration. Soluble protein (SP) content showed a decreasing pattern in general. Soluble sugar (SS)content increased under low concentration stress and decreased under high concentration stress.

Key words: NaCl stress, Nitraria tangutorum seedling, Antioxidants enzyme, Osmosis regulatory material

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