草地学报

草地学报 ›› 2014, Vol. 22 ›› Issue (4): 828-833.DOI: 10.11733/j.issn.1007-0435.2014.04.023

• 研究论文 • 上一篇    下一篇

60Co-γ辐射扁穗牛鞭草剂量筛选及表型变异研究

曾捷1, 黄琳凯1, 张新全1, 张瑜1, 张博涛1, 张婧1, 曹蔚1, 刘欢1, 严海东1   

  1. 四川农业大学草业科学系, 四川 雅安 625014
  • 收稿日期:2013-09-29 修回日期:2013-11-11 出版日期:2014-08-15 发布日期:2014-08-04
  • 通讯作者: 黄琳凯
  • 作者简介:曾捷（1992-），女，四川德阳人，学士，主要从事草类植物资源及育种研究，E-mail：zengjiezz@163.com
  • 基金资助:

    四川省育种攻关（2011YZGG-11）；国家现代牧草产业技术体（CARS-35-05）；四川农业大学大学生科研兴趣计划（2013020）资助

Study on Phenotype Mutation of Hemarthria compressa and Dose Selection of 60Co-γ Irradiation

ZENG Jie1, HUANG Lin-kai1, ZHANG Xin-quan1, ZHANG Yu1, ZHANG Bo-tao1, ZHANG Jing1, CAO Wei1, LIU Huan1, YAN Hai-dong1   

  1. Department of Grassland Science, Sichuan Agricultural University, Ya'an, Sichuan Province 625014, China
  • Received:2013-09-29 Revised:2013-11-11 Online:2014-08-15 Published:2014-08-04

PDF

摘要：

以牧草型‘雅安’扁穗牛鞭草（Hemarthria compressa‘Ya'an’）和草坪型‘H055’扁穗牛鞭草（H.compressa‘H055’）为材料，用60Co-γ射线分别照射种茎，研究其形态变异及变异的适宜射线剂量，旨在为扁穗牛鞭草辐射诱变育种提供基本信息。结果表明：诱变材料的存活率随辐照剂量增加而降低，‘雅安’扁穗牛鞭草种茎适宜诱变剂量为80~100Gy，‘H055’扁穗牛鞭草种茎适宜诱变剂量为70~90Gy；辐射当代材料在植株形态性状上发生了较明显变异，其变异频率分别为47.5%和37.5%。并获得75%叶片变小、株高变矮、茎变细的草坪型‘H055’扁穗牛鞭草优异种质和10%植株变高的牧草型‘雅安’扁穗牛鞭草突变体，表明辐射育种方法更适合于草坪型扁穗牛鞭草的选育。

关键词: 扁穗牛鞭草, 60Co-&gamma, 辐射诱变, 临界剂量, 半致死剂量, 表型变异

Abstract:

The phenotype mutation of Hemarthria compressa induced by 60Co-γ and the appropriate dosage of 60Co-γ used for irradiation were studied using both forage type H. compressa ‘Ya'an’ andturf type H. compressa ‘H055’ in order to provide basic information for mutation breeding. Results showed that the survival rates of individual plants decreased with the increasing of irradiation dosage. The appropriate dosage of 60Co-γ was 80~100 Gy for inducing H. compressa ‘Ya'an’ and 70~90 Gy for H. compressa‘H055’. The phenotypic variations of all tested materials were observed obviously and variation frequencies were 47.5% and 37.5%, respectively. The positive mutants of turf type H. compressa ‘H055’ with smaller leaves, more dwarf and slender stalks were obtained and accounted for 75% of all tested materials, while the positive mutants of forage type H. compressa ‘Ya'an’ accounted for 10%. These results indicate that radiation breeding method is more suitable for the turf type of H. compressa.

Key words: Hemarthria compressa, 60Co-&gamma, irradiation, Critical dose, LD50, Phenotypic variation

中图分类号: