草地学报

›› 2011, Vol. 19 ›› Issue (5): 795-802.DOI: 10.11733/j.issn.1007-0435.2011.05.014

• 研究论文 • 上一篇    下一篇

航天诱变紫花苜蓿第一代植株(SP1)表型变异及基因多态性分析

范润钧1,2, 邓波2, 陈本建1, 柴小琴3, 张文娟1   

  1. 1. 甘肃农业大学草业学院, 甘肃兰州730070;
    2. 中国农业大学动物科技学院草业科学系, 北京100193;
    3. 甘肃省天水市农科所, 甘肃天水741000
  • 收稿日期:2010-03-15 修回日期:2011-03-25 出版日期:2011-10-15 发布日期:2012-07-12
  • 通讯作者: 邓波,E-mail:dengbo67@cau.edu.cn
  • 作者简介:范润钧(1984- ),女,甘肃兰州人,硕士,研究方向为牧草育种,E-mail:105240523@qq.com
  • 基金资助:
    “十一五”国家科技支撑项目“牧草多因素诱变育种及聚合选育技术研究”(2008BADB3B04);农业部牧草种质资源保护(2009)资助

Phenotypic Variation and Gene Polymorphism of First Generation(SP1) of Spaceflight Carrying Alfalfa Seeds

FAN Run-jun1,2, DENG Bo2, CHEN Ben-jian1, CHAI Xiao-qin3, ZHANG Wen-juan1   

  1. 1. College of Grassland Science, Gansu Agricultural Univercity, Lanzhou, Gansu Province 730070, China;
    2. Departerment of Grassland Science, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
    3. Gansu Tianshui esearch Insititute of Agricultural Sciences, Tianshui, Gansu Province 741000, China
  • Received:2010-03-15 Revised:2011-03-25 Online:2011-10-15 Published:2012-07-12

PDF

摘要： 采用正交设计优化紫花苜蓿(Medicagosativa L.)SSR-PCR反应体系,从17对SSR引物中筛选出6对扩增产物具有稳定多态性的引物,检测第1代植株的基因多态性。结果表明:4个紫花苜蓿品种德福(Defi)、德宝(Derby)、阿尔刚金(Algonguin)、三得利(Sanditi)共检测到25个等位基因,每对引物检测出2～8个等位基因,平均为4.17个。结合植株较高、叶色较深、叶片较大、多叶4个表型突变指标,检测经过表型变异筛选的植株等位基因频率及每个位点的多态性信息量(PIC),PIC在0.2216～0.8328之间变化,平均为0.6366。分析多态基因植株与表型变异的相关性,根据检测结果初步确定了13株突变植株,为后继世代遗传变异的多代跟踪及选育奠定基础。

关键词: 航天诱变, 紫花苜蓿, 表型变异, 基因多态性

Abstract: To shorten breeding time and screen mutations,alfalfa(Medicago sativa L.) seeds were carried by spaceflight.Phenotypic variation and gene polymorphism of first generation(SP1) from the alfalfa seeds were analyzed using optimized SSR-PCR amplification system with orthogonal design.DNA polymorphism was detected by 6 SSR primers out of 17 SSR primers.Results show that 2 to 8 DNA bands were amplified by each primer and the average were 4.17.Total 25 DNA bands were amplified.Four characteristic indexes(plant height,leaf color,leaf size,leaf number) were measured.Polymorphism information content(PIC) changed from 0.2216 to 0.8328 and the average is 0.6366.These results preliminary confirm 13 mutations and provide foundation for selecting mutants’ phenotypic variation and genomic polymorphism.

Key words: Spaceflight mutation, Alfalfa, Phenotypic variation, Genomic polymorphism

中图分类号: