草地学报

草地学报 ›› 2015, Vol. 23 ›› Issue (5): 963-967.DOI: 10.11733/j.issn.1007-0435.2015.05.009

• 研究论文 • 上一篇    下一篇

NH4+-N对紫花苜蓿输导组织解剖结构的影响

郝凤, 刘晓静, 张晓玲   

  1. 甘肃农业大学草业学院草业生态系统教育部重点实验室中美草地畜牧业可持续发展研究中心, 甘肃 兰州 730070
  • 收稿日期:2015-04-30 修回日期:2015-08-31 出版日期:2015-10-15 发布日期:2015-12-01
  • 通讯作者: 刘晓静
  • 作者简介:郝凤(1985-),女,黑龙江鹤岗人,博士研究生,研究方向为牧草营养,E-mail:haofeng1026@163.com
  • 基金资助:

    国家自然科学基金项目(31460622);公益性行业(农业)科研专项(201403048-8)资助

The Effect of NH4+-N on Anatomical Structure of Alfalfa Conducting Tissue

HAO Feng, LIU Xiao-jing, ZHANG Xiao-ling   

  1. College of Grassland Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem of Ministry of Education, Sino-U. S. Centers for Grazing Land Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China
  • Received:2015-04-30 Revised:2015-08-31 Online:2015-10-15 Published:2015-12-01

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摘要：

以甘农3号紫花苜蓿(Medicago sativa 'Gannong No.3')为材料,采用石蜡切片技术和光学显微技术,比较研究了5个NH4+-N水平(0,105,210,315,420 mg·L-1)对紫花苜蓿根、茎和叶输导组织解剖结构的影响。结果表明:随着氮浓度的增加,贯通于根、茎、叶3个器官的维管组织先增大后减小,在NH4+-N浓度为210 mg·L-1时,维管束面积最大,木质部区域宽,韧皮部发达,导管数目多,其输导能力最强。此外,各营养器官的其他组织也发生了相应的改变,在0~210 mg·L-1随着氮浓度的增加,根皮层增厚,薄壁细胞体积减小;髓腔呈片状的髓组织,面积增大;叶主脉突起程度增大,表皮由长圆形排列更为紧密的单层细胞组成,在210 mg·L-1时处于最优状态,再提高浓度效果反而降低。

关键词: 紫花苜蓿, NH4+-N, 输导组织, 解剖结构, 维管束

Abstract:

Gannong NO.3 was used as experimental materials with five different NH4+-N concentrations(0,105,210,315,420 mg·L-1). Anatomical structure characteristics of three types of organs(roots, stems and leaflets)were observed by using paraffin section techniques and photomicrography. The results showed that vascular tissue throughout the roots,stems and leaflets was increased at the begining, and then reduced with the further increase of nitrogen treatment. When NH4+-N treatment was 210 mg·L-1, the function of conducting tissue achieved the best performance with more vessels, more multi-vascular bundles, larger and better developed xylem and phloem area as well as more xylem conduits. Changes in other tissues of vegetative organs were also observed, the roots cortex was thicked and parenchyma cell volume decreasesd. The intramedullary cavity had flaky tissue and the area was increased. Leaves midrib became more prominent and epidermis consisted of a layer of oblong, more compactly arranged epidermal cells with increased NH4+-N concentration within a range of 0 to 210 mg·L-1. The optimal concentration was at 210 mg·L-1. Further increase of the concentration of NH4+-N concentration reduced the improvement effects instead.

Key words: Alfalfa, NH4+-N, Conducting tissue, Anatomical structure, Palisade tissue

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