禾本科牧草及草坪草原生质体培养与融合技术研究进展

doi:10.11733/j.issn.1007-0435.2016.04.005

草地学报 ›› 2016, Vol. 24 ›› Issue (4): 738-746.DOI: 10.11733/j.issn.1007-0435.2016.04.005

禾本科牧草及草坪草原生质体培养与融合技术研究进展

牛奎举, 马晖玲

甘肃农业大学草业学院草业生态系统教育部重点实验室中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070

收稿日期:2015-03-18 修回日期:2016-02-29 出版日期:2016-08-15 发布日期:2016-11-29
通讯作者: 马晖玲,E-mail:mahl@gsau.edu.cn
作者简介:牛奎举(1991-),男,甘肃通渭人,博士研究生,主要研究方向为草种质资源及育种,E-mail:niukuiju@163.com
基金资助:
国家自然科学基金（草地早熟禾种间体细胞杂交的研究-31160482）资助

Research Progress in Protoplast Culture and Fusion Technologies of Gramineous Forage and Turfgrass Plants

NIU Kui-ju, MA Hui-ling

Pratacultural College, Gansu Agricultural University, Key laboratory of Grassland Ecosystem, Ministry of Education, Sino-U.S. Centers for Grazingland Ecosystem Sustainability, Lanzhou 730070, China

Received:2015-03-18 Revised:2016-02-29 Online:2016-08-15 Published:2016-11-29

摘要/Abstract

摘要：

禾本科牧草与草坪草在可持续农业发展、城市绿化、生态保护等方面起着至关重要的作用。植物体细胞杂交是有性杂交的重要补充，在转移由多基因控制和尚未克隆目标基因控制的性状方面具有极大的潜力。本文综述了禾本科牧草及草坪草的原生质体培养与融合技术，包括原生质体分离材料的选择、培养基成分、培养密度及培养方式、融合的方法与方式。同时，对禾本科牧草及草坪草体原生质体培养与融合技术的应用前景作出展望。

关键词: 禾本科牧草, 草坪草, 原生质体培养, 原生质体融合

Abstract:

Gramineous forage and turf grass plants play important roles in supporting the sustainable agriculture developments, afforestation of city and environmental protection. Somatic hybridization, which has great potential in transferring the target characters controlled by multigenes or uncloned genes, is an important supplement to the sexual hybridization. In this paper, the technologies of protoplast culture and fusion of gramineous forage and turf grass were reviewed. It included selecting the materials for protoplast isolation, culture media, culture density and methods, fusion methods. Moreover, the prospects of the protoplast culture and fusion technologies of gramineous forage and turf grass plants were made.

Key words: Gramineous forage plants, Turfgrass, Protoplast culture, Protoplast fusion

中图分类号:

牛奎举, 马晖玲. 禾本科牧草及草坪草原生质体培养与融合技术研究进展[J]. 草地学报, 2016, 24(4): 738-746.

NIU Kui-ju, MA Hui-ling. Research Progress in Protoplast Culture and Fusion Technologies of Gramineous Forage and Turfgrass Plants[J]. Acta Agrestia Sinica, 2016, 24(4): 738-746.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2016.04.005

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2016/V24/I4/738

参考文献

[1] 郭仰东,李蔚,李仁,等. 禾本科牧草和草坪草转基因育种研究进展[J]. 中国农业科学,2011,44(18):3812-3821
[2] Guo Y, Mizukami Y, Yamada T. Genetic characterization of androgenic progeny derived from Lolium perenne x Festuca pratensis cultivars[J]. New Phytologist,2005,166(2):455-464
[3] Eeckhaut T, Lakshmanan P S, Deryckere D, et al. Progress in plant protoplast research[J]. Planta,2013,238(6):991-1003
[4] Davey M R, Anthony P, Patel D, et al. Plant cell culture:essential methods[M]. New York:Wiley-Blackwell,2010:153-173
[5] Johnson A A,Veilleux R E. Somatic hybridization and applications in plant breeding[J]. Plant Breeding Reviews,2010,20:167-225
[6] 赵宏波,陈发棣,房伟民. 菊科植物原生质体融合研究进展[J]. 园艺学报,2006,33(4):908-914
[7] Xia G M. Progress of chromosome engineering mediated by asymmetric somatic hybridization[J]. Journal of Genetics and Genomics,2009,36(9):547-556
[8] Vasil V, Vasil I K. Isolation and culture of cereal protoplasts[J]. Theoretical and Applied Genetics,1980,56(3):97-99
[9] Lu C, Vasil V, Vasil I K. Isolation and Culture of Protoplasts of Panicum maximum Jacq. (Guinea Grass):Somatic Embryogenesis and Plantlet Formation[J]. Zeitschrift für Pflanzenphysiologie,1981,104(4):311-318
[10] Vasil V, Wang D, Vasil I K. Plant Regeneration From Protoplasts of Napier Grass (Pennisetum purpureum Schum.)[J]. Zeitschrift für Pflanzenphysiologie,1983,111(3):233-239
[11] Aftab F, Zafar Y, Malik K A, et al. Plant regeneration from embryogenic cell suspensions and protoplasts in sugarcane (Saccharum spp. hybrid cv. CoL-54)[J]. Plant Cell,Tissue and Organ Culture,1996,44(1):71-78
[12] Srinivasan C, Vasil I K. Plant Regeneration from Protoplasts of Sugarcane (Saccharum officinarum L.)[J]. Journal of Plant Physiology,1986,126(1):41-48
[13] Jones M G K, Dale P J. Reproducible Regeneration of Callus from Suspension Culture Protoplasts of the Grass Lolium multiflorum[J]. Zeitschrift für Pflanzenphysiologie,1982,105(3):267-274
[14] Folling M, Madsen S, Olesen A. Effect of nurse culture and conditioned medium on colony formation and plant regeneration from Lolium perenne protoplasts[J]. Plant Science,1995,108(2):229-239
[15] Horn M E, Conger B V, Harms C T. Plant regeneration from protoplasts of embryogenic suspension cultures of orchardgrass (Dactylis glomerata L.)[J]. Plant Cell Reports,1988,7(6):371-374
[16] 张根发,黄百渠,王丽,等. 大赖草悬浮细胞原生质体再生植株[J]. 植物学报,1993,35(6):422-428
[17] 陈东方,夏镇澳. 从棒头草(Polypogon fugax Nees ex Steud)原生质体培养获得再生成熟植株[J]. 中国科学(B辑),1987(1):54-60
[18] Stolarz A, Rz H L. Protoplast culture and transformation studies of Triticale (Triticosecale Wittmack)[J]. Plant Cell, Tissue and Organ Culture,1988,12(2):227-230
[19] Hahne B, Rz H L, Hahne G. Oat mesophyll protoplasts:their response to various feeder cultures[J]. Plant Cell Reports,1990,8(10):590-593
[20] Hahne B, Fleck J, Hahne G. Colony formation from mesophyll protoplasts of a cereal, oat[J]. Proceedings of the National Academy of Sciences,1989,86(16):6157-6160
[21] Zaghmout O M F, Torello W A. Isolation and culture of protoplasts from embryogenic suspension cultures of red fescue (Festuca rubva L.)[J]. Plant Cell Reports,1990,9(6):340-343
[22] 马晖玲,赵小强,白小明. 草地早熟禾午夜Ⅱ号原生质体培养及植株再生[J]. 草地学报,2010,18(1):103-107
[23] 赵小强,马晖玲,林栋,等. 草地早熟禾新格莱德胚性愈伤组织原生质体培养及植株再生的研究[J]. 草业学报,2010,19(2):55-60
[24] Nielsen K A, Knudsen E. Regeneration of green plants from embryogenic suspension on cultures of kentucky blue grass (Poa pratensis L.)[J]. Journal of Plant Physiology,1993,141(5):589-595
[25] 王铁邦,钱迎倩,李集临,等. 小偃麦原生质体培养及植株再生[J]. 植物学报,1990,32(5):329-336
[26] Inokuma C, Sugiura K, Cho C, et al. Plant regeneration from protoplasts of Japanese lawngrass[J]. Plant cell reports,1996,15(10):737-741
[27] Asano Y, Sugiura K. Plant regeneration from suspension culture-derived protoplasts of Agrostis alba L.(Redtop)[J]. Plant Science,1990,72(2):267-273
[28] Takamizo T, Suginobu K I, Ohsugi R. Plant regeneration from suspension culture derived protoplasts of tall fescue (Festuca arundinacea Schreb.) of a single genotype[J]. Plant science,1990,72(1):125-131
[29] Wang Z Y, Valles M P, Montavon P, et al. Fertile plant regeneration from protoplasts of meadow fescue (Festuca pratensis Huds.)[J]. Plant cell reports,1993,12(2):95-100
[30] Wang X, Rz H L. Plant regeneration from protoplasts of wild barley (Hordeum murinum L.)[J]. Plant Cell Reports,1994,13(3-4):139-144
[31] 戴雪梅,黄天带,孙爱花,等. 橡胶树原生质体培养研究进展[J]. 热带作物学报,2011,32(10):1973-1976
[32] 陈发棣,赵宏波,房伟民,等. 菊科植物原生质体研究进展[J]. 西北植物学报,2005,25(9):1913-1920
[33] Chabane D, Assani A, Bouguedoura N, et al. Induction of callus formation from difficile date palm protoplasts by means of nurse culture[J]. Comptes Rendus Biologies,2007,330(5):392-401
[34] 马晖玲,李玉珠. 植物原生质体培养的影响因素分析[J]. 甘肃农业大学学报,2007,42(4):38-43
[35] Gupta H S, Pattanayak A. Plant regeneration from mesophyll protoplasts of rice (Oryza sativa L.)[J]. Nature Biotechnology, 1993,11(1):90-94
[36] Sairam R V, Seetharama N, Devi P S, et al. Culture and regeneration of mesophyll-derived protoplasts of sorghum[Sorghum bicolor (L.) Moench] [J]. Plant Cell Reports,1999,18(12):972-977
[37] Kao K N, Michayluk M R. Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media[J]. Planta,1975,126(2):105-110
[38] 陈文品,吴琴,刘大钧,等. 黑麦草原生质体培养条件的初步研究[J]. 南京农业大学学报,1992,15(2):10-15
[39] 董晋江,夏镇澳. 谷子原生质体培养再生植株[J]. 科学通报,1990(7):538-540
[40] Grzebelus E, Szklarczyk M, Greń J, et al. Phytosulfokine stimulates cell divisions in sugar beet (Beta vulgaris L.) mesophyll protoplast cultures[J]. Plant Growth Regulation,2012,67(1):93-100
[41] Niewska E W, Majewska-Sawka A. Arabinogalactan-proteins stimulate the organogenesis of guard cell protoplasts-derived callus in sugar beet[J]. Plant Cell Reports,2007,26(9):1457-1467
[42] Riazunnisa K, Padmavathi L, Scheibe R, et al. Preparation of Arabidopsis mesophyll protoplasts with high rates of photosynthesis[J]. Physiologia Plantarum,2007,129(4):879-886
[43] Rákosy-Tican E, Aurori A, Vesa S, et al. In vitro morphogenesis of sunflower (Helianthus annuus) hypocotyl protoplasts:the effects of protoplast density,haemoglobin and spermidine[J]. Plant Cell,Tissue and Organ Culture,2007,90(1):55-62
[44] Potters G, Jansen M A K, Horemans N, et al. Dehydroascorbate and glutathione regulate the cellular development of Nicotiana tabacum L. SR-1 protoplasts[J]. In Vitro Cellular & Developmental Biology-Plant,2010,46(3):289-297
[45] Azad M A K, Yokota S, Ishiguri F, et al. Plant regeneration from mesophyll protoplasts of a medicinal plant,Phellodendron amurense rupr.[J]. In Vitro Cellular & Developmental Biology-Plant,2006,42(6):502-507
[46] Duquenne B, Eeckhaut T, Werbrouck S, et al. Effect of enzyme concentrations on protoplast isolation and protoplast culture of Spathiphyllum and Anthurium[J]. Plant Cell,Tissue and Organ Culture,2007,91(2):165-173
[47] Bajaj Y P S. Plant protoplasts and genetic engineering II[J]. Plant Growth Regulation,1991,10(2):173-174.
[48] Pati P K, Sharma M, Ahuja P S. Rose protoplast isolation and culture and heterokaryon selection by immobilization in extra thin alginate film[J]. Protoplasma,2008,233(1-2):165-171
[49] Sheahan M B, Rose R J, Mccurdy D W. Actin-filament-dependent remodeling of the vacuole in cultured mesophyll protoplasts[J]. Protoplasma,2007,230(3-4):141-152
[50] Pati P K, Sharma M, Ahuja P S. Extra thin alginate film:an efficient technique for protoplast culture[J]. Protoplasma,2005,226(3-4):217-221
[51] Grzebelus E, Szklarczyk M, Baranski R. An improved protocol for plant regeneration from leaf-and hypocotyl-derived protoplasts of carrot[J]. Plant Cell,Tissue and Organ Culture,2012,109(1):101-109
[52] Kowska A K, Adamus A. An alginate-layer technique for culture of Brassica oleracea L. protoplasts[J]. In Vitro Cellular & Developmental Biology-Plant,2012,48(2):265-273
[53] Tabaeizadeh Z, Ferl R J, Vasil I K. Somatic hybridization in the Gramineae:Saccharum officinarum L.(sugarcane) and Pennisetum americanum (L.) K. Schum.(pearl millet)[J]. Proceedings of the National Academy of Sciences,1986,83(15):5616-5619
[54] Ozias-Akins P, Ferl R J, Vasil I K. Somatic hybridization in the gramineae:Pennisetum americanum (L.) K. Schum. (Pearl millet)+Panicum maximum Jacq. (Guinea grass)[J]. Molecular and General Genetics MGG,1986,203(3):365-370
[55] Xia G M, Chen H M. Plant regeneration from intergeneric somatic hybridization between Triticum aestivum L. and Leymus chinensis (Trin.) Tzvel[J]. Plant Science,1996,120(2):197-203
[56] Xia G M, Li Z Y, Wang S L, et al. Asymmetric somatic hybridization between haploid common wheat and UV-irradiated Haynaldia villosa[J]. Plant Science,1998,137(2):217-223
[57] 夏光敏,向凤宁,周爱芬,等. 小麦与高冰草属间体细胞杂交获可育杂种植株[J]. 植物学报,1999,41(4):9-12
[58] 向凤宁,夏光敏,周爱芬,等. 普通小麦与无芒雀麦不对称体细胞杂交的研究[J]. 植物学报,1999,41(5):9-13
[59] Xiang F, Xia G, Chen H. Asymmetric somatic hybridization between wheat (Triticum aestivum) and Avena sativa L.[J]. Science in China Series C:Life Sciences,2003,46(3):243-252
[60] Feng D, Xia G, Zhao S, et al. Two quality-associated HMW glutenin subunits in a somatic hybrid line between Triticum aestivum and Agropyron elongatum[J]. Theoretical and Applied Genetics,2004,110(1):136-144
[61] Li C L, Xia G M. Asymmetric somatic hybridization between mixed wheat and Psathyrostachys juncea[J]. Chinese journal of biotechnology,2004,20(4):610-614.
[62] Ge T M, Lin X H, Qin F L, et al. Protoplast electrofusion between common wheat (Triticum aestivum L.) and Italian ryegrass (Lolium multiflorum Lam.) and regeneration of mature cybrids[J]. In Vitro Cellular & Developmental Biology-Plant,2006,42(2):179-187.
[63] Cai Y, Xiang F, Zhi D, et al. Genotyping of somatic hybrids between Festuca arundinacea Schreb. and Triticum aestivum L[J]. Plant Cell Reports,2007,26(10):1809-1819
[64] Liu C, Li S, Wang M, et al. A transcriptomic analysis reveals the nature of salinity tolerance of a wheat introgression line[J]. Plant Molecular Biology,2012,78(1-2):159-169
[65] Takamizo T, Spangenberg G, Suginobu K, et al. Intergeneric somatic hybridization in Gramineae:somatic hybrid plants between tall fescue (Festuca arundinacea Schreb.) and Italian ryegrass (Lolium multiflorum Lam.)[J]. Molecular and General Genetics,1991,231(1):1-6
[66] Spangenberg G, Valles M P, Wang Z Y, et al. Asymmetric somatic hybridization between tall fescue (Festuca arundinacea Schreb.) and irradiated Italian ryegrass (Lolium multiflorum Lam.) protoplasts[J]. Theoretical and applied genetics,1994,88(5):509-519
[67] Spangenberg G, Wang Z Y, Legris G, et al. Intergeneric symmetric and asymmetric somatic hybridization in Festuca and Lolium[J]. Euphytica,1995,85(1-3):235-245
[68] Creemers-Molenaar J, Hall R D, Krens F A. Asymmetric protoplast fusion aimed at intraspecific transfer of cytoplasmic male sterility (CMS) in Lolium perenne L[J]. Theoretical and applied genetics,1992,84(5-6):763-770
[69] 梁雪莲,陈平,佘伟标,等. 匍匐翦股颖和狗牙根原生质体融合研究[J]. 中国草地学报,2009,31(4):63-68
[70] 赵小强,马晖玲,周万海,等. 草地早熟禾原生质体培养与融合[J]. 核农学报,2010,24(4):737-743
[71] Power J B, Cummins S E, Cocking E C. Fusion of isolated plant protoplasts[J]. Nature,1970,225(5237):1016-1018
[72] Kao K N, Michayluk M R. A method for high-frequency intergeneric fusion of plant protoplasts[J]. Planta,1974,115(4):355-367
[73] Sencia M, Takeda J, Abe S, et al. Induction of cell fusion of plant protoplasts by electrical stimulation[J]. Plant and cell physiology,1979,20(7):1441-1443
[74] Olivares-Fuster O, Duran-Vila N, Navarro L. Electrochemical protoplast fusion in citrus[J]. Plant Cell Reports,2005,24(2):112-119
[75] 戴雪梅,黄天带,孙爱花,等. 植物原生质体融合研究进展及其在育种中的应用[J]. 热带作物学报,2012,33(8):1516-1521
[76] Melchers G, Sacristán M D, Holder A A. Somatic hybrid plants of potato and tomato regenerated from fused protoplasts[J]. Carlsberg Research Communications,1978,43(4):203-218
[77] 谢从华,柳俊. 植物细胞工程[M]. 北京:高等教育出版社,2004,180-204
[78] 徐小勇,孔芬,王汝艳,等. 植物亚原生质体分离及融合研究进展[J]. 中国生物工程杂志,2009(9):97-101
[79] Spangenberg G, Freydl E, Osusky M, et al. Organelle transfer by microfusion of defined protoplast-cytoplast pairs[J]. Theoretical and Applied Genetics,1991,81(4):477-486
[80] Xu X Y, Liu J H, Deng X X. Isolation of cytoplasts from Satsuma mandarin (Citrus unshiu Marc.) and production of alloplasmic hybrid calluses via cytoplast-protoplast fusion[J]. Plant Cell Reports,2006,25(6):533-539
[81] Sigareva M A, Earle E D. Direct transfer of a cold-tolerant Ogura male-sterile cytoplasm into cabbage (Brassica oleracea ssp. capitata) via protoplast fusion[J]. Theoretical and Applied Genetics,1997,94(2):213-220
[82] 夏光敏. 山融3号[J]. 中国农业信息.2009(8):38
[83] Hall R D, Krens F A, Rouwendal G J. DNA radiation damage and asymmetric somatic hybridization:Is UV a potential substitute or supplement to ionising radiation in fusion experiments[J]. Physiologia Plantarum,1992,85(2):319-324
[84] 付春华,余龙江. 细胞融合与植物细胞质雄性不育性状改良[J]. 植物学通报,2005(22):99-103
[85] 杨明亮,王继安. 原生质体融合技术在大豆遗传改良上的应用[J]. 大豆科技,2012(2):13-16
[86] Sundberg E, Glimelius K. A method for production of interspecific hybrids within Brassiceae via somatic hybridization, using resynthesis of Brassica napus as a model[J]. Plant science,1986,43(2):155-162
[87] Wu J, Ferguson A R, Mooney P A. Allotetraploid hybrids produced by protoplast fusion for seedless triploid Citrus breeding[J]. Euphytica,2005,141(3):229-235

禾本科牧草及草坪草原生质体培养与融合技术研究进展

Research Progress in Protoplast Culture and Fusion Technologies of Gramineous Forage and Turfgrass Plants

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	杜建雄, 任尉香, 袁涓文, 黄慧琼, 刘杰, 肖玉. 汞胁迫对4个草坪草、牧草品种幼苗生长和生理的影响[J]. 草地学报, 2021, 29(8): 1712-1718.
[2]	谢开云, 孟翔, 徐珍珍, 张力文, 万江春, 颜安, 李陈建. 新疆半干旱地区不同种类混播草地的牧草产量和营养价值研究[J]. 草地学报, 2021, 29(8): 1835-1842.
[3]	王晶, 伏兵哲. 基于文献计量分析的禾本科牧草染色体加倍研究进展[J]. 草地学报, 2021, 29(3): 413-424.
[4]	张海娟, 芦光新, 范月君, 周华坤, 周学丽, 窦声云, 颜珲璘, 马坤, 赵阳安. 不同染色方法对两种禾本科牧草菌根侵染率的影响[J]. 草地学报, 2021, 29(12): 2838-2844.
[5]	李钰莹, 刘娜, 荆则尧, 温伟, 曹磊, 夏方山, 朱慧森. 黄花蒿浸提液对4种草坪草种子萌发及幼苗生长的影响[J]. 草地学报, 2020, 28(5): 1285-1293.
[6]	崔会婷, 孙熙喏, 马承泽, 胡倩楠, 孙彦. 代谢组学在牧草与草坪草抗逆性中的研究进展[J]. 草地学报, 2020, 28(4): 873-880.
[7]	纪童, 王波, 王占军, 杨军银, 李强, 刘志刚, 关文昊, 何国兴, 潘冬荣, 柳小妮. 草坪草叶绿素高光谱估测模型的建立及Pb²⁺胁迫下的反演研究[J]. 草地学报, 2020, 28(3): 675-683.
[8]	赵娜, 徐庆国, 苏鹏, 梁东鸣, 唐瑶. 7个暖季型草坪草品种对低温胁迫的抗性差异研究[J]. 草地学报, 2020, 28(2): 375-382.
[9]	李京蓉, 马真, 张骞, 刘泽华, 张春辉, 乔安海, 邓艳芳, 王文颖, 邵新庆, 姚步青, 黄小涛, 周华坤. 青海省6种禾本科牧草的抗寒性研究及综合评价[J]. 草地学报, 2020, 28(2): 405-411.
[10]	赵海欣, 朱晓艳, 崔亚垒, 王成章, 李德锋. 禾本科草坪草混播天蓝苜蓿、小苜蓿的效果研究[J]. 草地学报, 2018, 26(5): 1229-1234.
[11]	宋娅丽, 王克勤, 王莎, 钱夏吉, 徐璟. 3种冷季型草坪草对持续干旱、高温及其互作的生理生态响应[J]. 草地学报, 2018, 26(3): 705-717.
[12]	李莲香. 四种多年生禾本科牧草引种试验[J]. 草地学报, 2018, 26(1): 264-267.
[13]	刘佳星, 王雷, 呼丽瑶. 野生羊茅与引进羊茅‘猎狗5号’竞争特性的研究[J]. 草地学报, 2017, 25(6): 1352-1361.
[14]	郭明英, 吴艳玲, 徐丽君, 张木兰, 乌兰, 吕潇潇, 吕世杰. 呼伦贝尔地区6种禾本科牧草养分对应分析[J]. 草地学报, 2017, 25(5): 1160-1164.
[15]	郭志鹏, 宗士寅, 张靖雪, 王苗利, 曲根, 管永卓, 严学兵, 郭玉霞. 郑州地区3种冷季型草坪草根部入侵真菌鉴定[J]. 草地学报, 2017, 25(3): 523-528.