草地学报 ›› 2022, Vol. 30 ›› Issue (3): 587-593.DOI: 10.11733/j.issn.1007-0435.2022.03.010

• 研究论文 • 上一篇    

叠氮化钠诱变燕麦M1代的主要性状分析

蔺豆豆, 赵桂琴, 柴继宽, 宫文龙, 苏玮娟, 张丽睿   

  1. 甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 甘肃省草业工程实验室, 中-美草地畜牧业可持续发展研究中心, 甘肃 兰州 730000
  • 收稿日期:2022-01-03 修回日期:2022-02-19 发布日期:2022-03-30
  • 通讯作者: 赵桂琴,E-mail:zhaogq@gsau.edu.cn
  • 作者简介:蔺豆豆(1995-),女,汉族,甘肃敦煌人,硕士研究生,主要从事牧草种质资源及育种研究,E-mail:1843118097@qq.com
  • 基金资助:
    甘肃省重大专项(19ZD2NA002-3-1);财政部和农业农村部:国家现代农业产业技术体系(CARS-07-C-1)资助

Analysis of Main Agronomic Traits of M1 Generation of Oat Mutagenized by NaN3

LIN Dou-dou, ZHAO Gui-qin, CHAI Ji-kuan, GONG Wen-long, SU Wei-juan, ZHANG Li-rui   

  1. College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province/Sino-U. S. Centers for Grazingland Ecosystem Sustainability, Lanzhou, Gansu Province 730000, China
  • Received:2022-01-03 Revised:2022-02-19 Published:2022-03-30

摘要: 为明确叠氮化钠(NaN3)处理后燕麦(Avena sativa) M1代植株主要性状的变化,本研究以两个燕麦品种‘爱沃’(‘Everleaf’)和‘青永久709’(‘Qingyongjiu 709’)种子为材料,设置不同叠氮化钠浓度(0,5,10,15,20mM)和不同处理时间(1,2,3h),将处理后的种子播到田间,观察M1代植株的农艺性状,并对主要性状进行测定分析。结果表明,NaN3处理后燕麦M1代的生育期较对照推迟2~5d,株高和主穗粒数明显下降且‘青永久709’的降幅更大,在15mM/3h处理下,与对照相比,其株高和主穗粒数分别下降了24.06%和76.69%。另外,两品种燕麦M1代旗叶长也低于对照,但旗叶宽却高于对照,从而对旗叶面积造成影响。其中‘青永久709’的M1代旗叶面积总体大于对照,但‘爱沃’平均减小了7.23%。NaN3处理后燕麦M1代各指标均发生了不同程度的变化,其中有效分蘖数和主穗粒重的变化最大。‘爱沃’和‘青永久709’的M1代主穗粒重最大变异系数分别为87.92%和90.80%。主成分分析表明,两品种被清晰地划分为两个彼此独立的类群,不同品种的诱变群体也分属不同的亚群,且‘青永久709’的M1代各指标的变化幅度总体大于‘爱沃’。

关键词: 燕麦, 化学诱变, 叠氮化钠, 诱变一代, 性状

Abstract: In order to clarify the changes of main agronomic traits of oat (Avena sativa) M1 generation plants after different sodium azide (NaN3) treatments,the seeds of two oat varieties ‘Everleaf’ and ‘Qingyongjiu 709’were used as materials. Set different NaN3 concentrations (0,5,10,15,20 mM) and different treatment time (1,2,3 h),and the treated seeds were sown in the field to observe the agronomic traits of the M1 generation plants,and the main traits were measured and analyzed. The results showed that the growth period of oat M1 generation after NaN3 treatment was delayed by 2~5 d compared with the control,and the plant height and grain number of the main panicle decreased significantly,and the decline of ‘Qingyongjiu 709’ was more significant. Under 15 mM/3 h treatment,the plant height and grain number of the main panicle decreased by 24.06% and 76.69% compared with the control,respectively. In addition,the flag leaf length of the two varieties of oat M1 generation also decreased compared with the control,but the flag leaf width increased,which affected the flag leaf area. Among them,the flag leaf area of M1 generation of ‘Qingyongjiu 709’ increased as a whole compared with the control,but ‘Everleaf’ decreased by 7.23% on average. After NaN3 treatment,the indexes of oat M1 generation changed in varying degrees,among which the number of effective tillers and grain weight of the main panicle changed the most. The maximum coefficient of variation of main panicle grain weight in M1 generation of ‘Everleaf’ and ‘Qingyongjiu 709’ were 87.92% and 90.80% respectively. PCA analysis showed that the two varieties were clearly divided into two independent groups,the mutagenic groups of different varieties also belonged to different subgroups,and the variation range of each index of the M1 generation of ‘Qingyongjiu 709’ was generally greater than that of ‘Everleaf’.

Key words: Oats, Chemical mutagenesis, Sodium azide, M1 generation, Traits

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