关键词: 豌豆蚜, 次生共生菌, 有性世代, 发育, 繁殖

Abstract: To clarify the effects of bacterial secondary symbionts on the biological characteristics of sexual generations of Acyrthosiphon pisum red morph,A. pisum red morph infected with both Hamiltonella and Regiella were used as initial aphids. First,clones uninfected with bacterial secondary symbionts were obtained using the antibiotic feeding method,then 3 artificially infected clones were prepared. Each infected clone was placed under a short daylight environment to reveal the sex ratio and then 9 different mating treatments all of which were sexual aphids were prepared and after mating the longevity of the sexual aphids,reproduction and fertilisation success rate of each mating treatment were counted,and the incubation period and hatching rate of fertilised eggs were further investigated. The results showed that the sex ratio becomes female biased and the longevity of sexual aphids reduced when infected with Hamiltonella or Regiella. Infection of oviparous females reduced the number of eggs,while infection of males resulted in reduced fertilisation rates. Whether the fertilised eggs infected with bacterial secondary symbionts or not had no significant effect on the hatching calendar,however,the hatching rate of fertilised eggs infected with bacterial secondary symbionts was significantly higher than that of uninfected eggs. There was no additive effect after simultaneous infection with Hamiltonella and Regiella. These results indicate that Hamiltonella and Regiella had a negative effect on the longevity of sexual aphids of the A. pisum red morph clone,however,their ability to enhance host fitness by regulating the sex ratio and increasing the hatching rate of fertilised eggs. There might be a competitive effect between Hamiltonella and Regiella.

Key words: Acyrthosiphon pisum, Bacterial secondary symbionts, Sexual generation, Development, Reproduction