草地学报

›› 1997, Vol. 5 ›› Issue (3): 201-204.DOI: 10.11733/j.issn.1007-0435.1997.03.010

• 研究论文 • 上一篇    下一篇

新麦草幼穗组织培养及再生植株的研究

王淑强, 王善敏, 刘玉红   

  1. 中国科学院国家计委自然资源综合考察委员会, 北京 100010
  • 出版日期:1997-08-15 发布日期:1997-08-15

A Study on Tissue Culture and Plant Regeneration of Psathyrostachys perennis Keng

Wang Shuqiang, Wang Shanmin, Liu Yuhong   

  1. Commission for Integrated Survey of Natural Resources, CAS Beijing 100010
  • Online:1997-08-15 Published:1997-08-15

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摘要： 本文通过组织培养研究不同激素组合和浓度对新麦草助穗愈伤组织的诱导、分化和植株再生的影响。结果表明,在MS附加2,4－D(3mg/L)和6－BA(0.2mg/L)的培养条件下,可从新麦草幼德中获得较高的愈伤组织诱导率和分化率,植株再生率可达80％以上。

关键词: 新麦草, 组织培养, 植株再生

Abstract: Psathyrostachys perennis Keng adapted in semi-arid regions is a good grass resources for its high yield, fine quality and tolerant to harsh stress.In this paper, we have studied the effects of differen hormone combinations and concentrations on callus induchon and plant regeneration of Psathyrostachys perennis Keng.The experimental results showed that the higher rate of callus induction and differentiation were obtained from young inflorescences on MS medium containing 2,4-D(3mg/L)and 6-BA(0.2 mg/L).The rate of plant regeneration can reach to over 80%.

Key words: Psathyrostachys perennis Keng, Tissue culture, Plant regeneration