草地学报

›› 2000, Vol. 8 ›› Issue (3): 233-236.DOI: 10.11733/j.issn.1007-0435.2000.03.012

• 研究报告 • 上一篇    

披碱草和野大麦及其杂种F1与BC1酯酶同工酶分析

李造哲, 云锦凤, 马青枝, 于卓   

  1. 内蒙古农业大学, 呼和浩特, 010019
  • 收稿日期:2000-03-09 修回日期:2000-07-05 出版日期:2000-08-15 发布日期:2000-08-15

Analysis of Esterase Isozymes of Elymus dahuricus,Hordeum brevisubulatum Their Hybrid F1 and BC1

Li Zaozhe, Yun Jinfeng, Ma Qingzhi, Yu Zhuo   

  1. Inner Mongolia Agricultural University, Huhhot, 010019
  • Received:2000-03-09 Revised:2000-07-05 Online:2000-08-15 Published:2000-08-15

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摘要： 采用聚丙烯酰胺垂直平板电泳技术对披碱草和野大麦及其杂种F1与回交1代酯酶同工酶进行比较分析。结果表明,亲本披碱草和野大麦有位点相同的9条基带,从酶蛋白分子水平验证,两亲本有较近的亲缘关系。杂种F1的酯酶同工酶谱主要表现为双亲酶带的互补类型,亲本的个别酶带在杂种F1有丢失现象,正反交杂种F1均产生一条杂种带。杂种F1酶谱有偏向母本遗传的倾向。正反交的回交1低酯酶同工酶谱基本相同,但也存在一定的差异,回交1代的酯酶同工酶谱明显地偏向轮回亲本野大麦。酯酶同工酶具有多态性,可作为遗传标记用于杂种鉴定和回交后代目标性状植株的检测。

关键词: 披碱草, 野大麦, 杂种F1, 回交1代(BC1), 酯酶同工酶

Abstract: The esterase(EST)isozymes of Elymus dahuricus,Hordeum brevisubulatum,their Hybrid F1 and BC1 were analyzed by polyacrylamid gel electrophoresis.The results showed that toth E.dahuricus and H.brevisubulatum had 9 base bands at same location,whhich meant that the relationship between E.dahuricus and H.brevisubulatum was closer;the zymogram types of the hybrid F1 were mainly the mutual suplementary isozymes of the parents,very few isozyme bands of the parents were lost,the hybrid F1 had a hybrid band,the zymograms of the hybrid F1 showed a tendency toward the maternal type;there were no significant differences in EST zymograms between H.brevisubulatum×E.dahuricus×H.brevisubulatum(BC1)and E.dahuricus×H.brevisubulatum×H.brevisubulatum(BC1),but some differences existed,the zymograms of BC1 showed a tendency toward the recurrent parent H.brevisublatum obviously.There were polymorphisms in isozymes among the parents,their hybrid F1 and BC1,which can be used as genetic marker to identify hybrid and determine the plants with object characteristics in backcross desendants.

Key words: Elymus dahuricus, Hordeum brevisubulatum, Hybrid F1, Backcross1(BC1), Esterae isozyme

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