苜蓿青贮接种菌系Li6、Al2、Ru2的筛选及其发酵特性

doi:10.11733/j.issn.1007-0435.2004.02.010

›› 2004, Vol. 12 ›› Issue (2): 124-128.DOI: 10.11733/j.issn.1007-0435.2004.02.010

苜蓿青贮接种菌系Li6、Al2、Ru2的筛选及其发酵特性

王小芬, 崔宗均, 胡跃高, 陈晓燕, 高丽娟, 张涛

中国农业大学农学与生物技术学院, 北京, 100094

收稿日期:2003-03-27 修回日期:2004-04-02 出版日期:2004-05-15 发布日期:2004-05-15
通讯作者: 崔宗均
作者简介:王小芬(1976- ),女,博士研究生,主要从事微生物群体功能及有机物资源再利用研究
基金资助:
农业部跨越计划项目"青刈黑麦、苜蓿产业化生产技术集成试验示范"(2000-24)

Selection of Inoculants of Li6、Al2、Ru2 for Alfalfa Silage and Analysis of Their Fermented Characteristics

WANG Xiao-fen, CUI Zong-jun, HU Yao-gao, CHEN Xiao-yan, GAO Li-juan, ZHANG Tao

College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China

Received:2003-03-27 Revised:2004-04-02 Online:2004-05-15 Published:2004-05-15

摘要/Abstract

摘要： 以pH值的降低迅速、稳定,L(+)乳酸积累量多为指标,通过连续的限制性培养,从发酵饲料、青贮苜蓿及乳酸菌制剂中分别筛选出Li6、Al2和Ru2三组菌系,以期作为苜蓿青贮用菌剂。该三组菌系均pH下降迅速,发出香甜气味,在玉米粉培养基、苜蓿汁培养基、MRS培养基中将pH稳定到3.8～4.0之间,所需时间分别为10h、16h和20h。气质联机分析结果表明,发酵液中含有较高的乙酸、乳酸,还有少量甲酸、丙酸等挥发性脂肪酸及丁二醇、乙酸乙酯、异丁醛、乙醛和乙酰胺等风味物质;在代谢产物中乙酸和乳酸占总代谢物的98%以上,其中L(+)乳酸的比率高达60%～70%。

关键词: 草原学, 苜蓿, 青贮, 菌系, 筛选, 发酵特性

Abstract: Aiming at a silage inoculant that could quickly debase and stabilize the pH value of fresh grass, and add to it more L(+) lactic acid, a continuous restricted cultivation of the material was carried on until three groupsof microbial community-Li6, A12-, Ru2-have been selected from fermented forage, a lfalfa silage and a lactic bacteria agent. The three groups of microbial community alignith the target: debasing the pH value quickly and inducing the silage to emit a sugary fragrance. Uultured in corn medium, alfalfa medium, and MRS,the three groups of microbial community cause the mediums’pH value to debase to 3.8~4.0 within 10 hours, 16 hours, and 20 hours respectively. Through GC-MS analysis, large amount of metabolic products such as lactic acid and acetic acid, and some formic acid and propionic acid are obtained. Other materials like butanediol, ethyl acetate, acetaldehyde, glycerol are also produced. Among the metabolic products,lactic acid and acetic acid take 98% of the total, in which 60%~70% is L(+) lactic acid.

Key words: Grassland science, Alfalfa, Silage, Microbial community, Selection, Fermenting characteristic

中图分类号:

Q815
S816.53

王小芬, 崔宗均, 胡跃高, 陈晓燕, 高丽娟, 张涛. 苜蓿青贮接种菌系Li6、Al2、Ru2的筛选及其发酵特性[J]. , 2004, 12(2): 124-128.

WANG Xiao-fen, CUI Zong-jun, HU Yao-gao, CHEN Xiao-yan, GAO Li-juan, ZHANG Tao. Selection of Inoculants of Li6、Al2、Ru2 for Alfalfa Silage and Analysis of Their Fermented Characteristics[J]. , 2004, 12(2): 124-128.

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苜蓿青贮接种菌系Li6、Al2、Ru2的筛选及其发酵特性

Selection of Inoculants of Li6、Al2、Ru2 for Alfalfa Silage and Analysis of Their Fermented Characteristics

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