草地学报

›› 2004, Vol. 12 ›› Issue (3): 223-226.DOI: 10.11733/j.issn.1007-0435.2004.03.012

• 研究论文 • 上一篇    下一篇

红豆草空间诱变突变体叶片同工酶及细胞超微结构分析

张蕴薇1, 任卫波1, 刘敏2, 韩建国1, 舒彬1, 陆伟1   

  1. 1. 中国农业大学草业科学系, 北京, 100094;
    2. 中国科学院遗传与发育研究所, 北京, 100101
  • 收稿日期:2004-06-07 修回日期:2004-08-26 出版日期:2004-08-15 发布日期:2004-08-15
  • 作者简介:张蕴薇(1974- ),女,蒙古族,博士,讲师,从事牧草育种研究,E-mail:zywei@cau.edu.cn
  • 基金资助:
    农业部"948"项目"退化草地综合改良与草地建设配套技术"(202099)

Study on Peroxidase and Esterase Isoenzymes and the Ultrastructure of the Space Induced Mutant Leaves of Sainfoin(Onobrychis viciaefolia Scop.)

ZHANG Yun-wei1, REN Wei-bo1, LIU Min2, HAN Jian-guo1, SHU Bin1, LU Wei1   

  1. 1. Institute of Grassland Science, China Agricultural University, Beijing, 10094, China;
    2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China
  • Received:2004-06-07 Revised:2004-08-26 Online:2004-08-15 Published:2004-08-15

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摘要： 经神州4号飞船搭载的红豆草种子,当代出现匍匐型突变体,其叶片同工酶谱及细胞超微结构的分析结果表明:匍匐型突变体、空间诱变直立型植株及对照之间过氧化物酶同工酶酶带数没有明显差异,其中匍匐型突变体脂酶同工酶酶带数与对照相同,空间诱变直立型植株酶带数多于对照;空间诱变的红豆草,叶片细胞壁不规则增厚,细胞质稀薄,液泡大,叶绿体变小,形状多不规则,叶绿体内淀粉粒细小、数量多,基粒片层直径小,但数量明显多于对照,匍匐型突变体表现尤为明显。

关键词: 红豆草, 空间诱变, 过氧化物酶同工酶, 脂酶同工酶, 超微结构

Abstract: Some of the sainfoin (Onobrychis viciaefolia Scop.)seeds returning from a trip to space with the airship "ShenZhou NO.4" sprouted creepy mutants. An analysis of the peroxidase and esterase isoenzymes and the ultrastructure of the leaves of the mutants, those of the space-travelled but stand-up sainfoin plants, and those of the control, was carried out. It shows that all the three examined types have similar band number of peroxidase isoenzymes. The mutant and the control give the same band number of esterase isoenzymes, while the stand-up sainfoin possesses more band number of esterase isoenzymes than the other two. It also shows that, compared to the ultrastructure of the control, that of the two types of space induced sainfoin plants, more particularly the creepy mutant, exhibit the following abnormalities: irregularly thickening of the cell wall, thinning of the cytoplasm, bigger vacuoles, smaller chloroplasts of irregular shapes, smaller but more numerous amyloid granules contained in chloroplasts, and shorter diameter of stroma thylakoid membranes but more membrane layers.

Key words: Sainfoin(Onobrychis viciaefolia Scop.), Space induced, Peroxidase, Esterase, Ultrastructure

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