草地学报

›› 2007, Vol. 15 ›› Issue (1): 66-69.DOI: 10.11733/j.issn.1007-0435.2007.01.012

• 研究论文 • 上一篇    下一篇

柠条锦鸡儿的组织培养

宋俊双, 王赞, 孙桂芝, 高洪文   

  1. 中国农业科学院北京畜牧兽医研究所, 北京, 100094
  • 收稿日期:2006-08-08 修回日期:2006-11-27 出版日期:2007-02-15 发布日期:2007-02-15
  • 通讯作者: 王赞,E-mail:gaohongwen@263.net
  • 作者简介:宋俊双(1980- ),女,河南人,硕士,主要从事牧草种质资源研究,E-mail:songjs2004@yahoo.com.cn
  • 基金资助:
    国家“863”项目(2002AA241091)

Study on the Tissue Culture of Horqin Peashrub

SONG Jun-shuang, WANG Zan, SUN Gui-zhi, GAO Hong-wen   

  1. Institute of Animal Science, Chinese Academy of Agriculture Science, Beijing 100094, China
  • Received:2006-08-08 Revised:2006-11-27 Online:2007-02-15 Published:2007-02-15

PDF

摘要： 以柠条锦鸡儿(Caragana korshinskii Kom.)无菌苗的子叶和下胚轴为外植体,研究其愈伤组织诱导及植株再生。结果表明:柠条锦鸡儿子叶和下胚轴均能诱导出愈伤组织,其适宜诱导培养基分别为M S+2,4-D2.0(mg/L)+KT0.1(mg/L)和M S+2,4-D2.0(mg/L)+BA0.5(mg/L);子叶诱导不定芽的适宜培养基为M S+6-BA0.2(mg/L)+NAA0.05(mg/L),下胚轴诱导不定芽的适宜培养基为MS+6-BA0.5(mg/L)+NAA0.05(mg/L);适宜生根培养基为1/2M S+IBA0.5(mg/L)+NAA0.5(mg/L)。本试验结果可为柠条锦鸡儿的快速繁殖利用及品种改良提供依据。

关键词: 柠条锦鸡儿, 组织培养, 外殖体, 激素浓度

Abstract: A study on callus induction and plantlet regeneration of Horqin peashrub(Caragana korshinskii Kom.) was carried out.The results show that both the cotyledon and hypocotyl of the plant could successfully induce callus,for which the optimum media were MS+2,4-D 2.0(mg/L)+KT 0.1(mg/L),and MS+2,4-D 2.0(mg/L)+BA 0.5(mg/L),respectively.The best media for inducing adventitious buds by cotyledon and hypocotyl were MS+6-BA 0.2(mg/L)+NAA 0.05(mg/L),and MS+6-BA 0.5(mg/L)+ NAA 0.05(mg/L),respectively,while the optimum rooting medium was 1/2MS+IBA 0.5(mg/L)+ NAA 0.5(mg/L).Some suggestions for the rapid propagation and variety improvement of Caragana korshinskii are offered in this study.

Key words: Horqin peashrub(Caragana korshinskii), Tissue culture, Callus, Concentrations of hormones

中图分类号: