多裂骆驼蓬水浸液对多年生黑麦草的化感作用与生理生化表现

doi:10.11733/j.issn.1007-0435.2008.04.011

›› 2008, Vol. 16 ›› Issue (4): 374-379.DOI: 10.11733/j.issn.1007-0435.2008.04.011

多裂骆驼蓬水浸液对多年生黑麦草的化感作用与生理生化表现

刘建新¹, 胡浩斌², 王鑫¹

1. 陇东学院生命科学系, 甘肃, 庆阳, 745000;
2. 陇东学院化学与化工学院, 甘肃, 庆阳, 745000

收稿日期:2007-10-10 修回日期:2008-04-15 出版日期:2008-08-15 发布日期:2008-08-15
作者简介:刘建新(1964- ),男,甘肃通渭人,副教授,主要从事植物生理生态研究,E-mail:liujx1964@163.com
基金资助:
甘肃省教育厅科研基金项目(JY0510-07)

Study on Allelopathy of Aqueous Extract from Peganum multisectum (Maxim.) Bobr. on Perennial Ryegrass (Lolium perenne L.) and Its Physiological-biochemical Manifestation

LIU Jian-xin¹, HU Hao-bin², WANG Xin¹

1. Department of Life Science, Longdong University, Qingyang, Gansu Province 745000, China;
2. College of Chemistry & Chemical Engineering, Longdong University, Qingyang, Gansu Province 745000, China

Received:2007-10-10 Revised:2008-04-15 Online:2008-08-15 Published:2008-08-15

摘要/Abstract

摘要： 为探讨多裂骆驼蓬(Peganum multisectum (Maxim.) Bobr.)对多年生黑麦草(Lolium perenne L.)的化感效应,采用溶液培养和盆栽土培法研究了不同浓度多裂骆驼蓬水浸液对多年生黑麦草种子萌发和幼苗生长的影响及其生理生化表现。结果表明,多裂骆驼蓬水浸液显著抑制多年生黑麦草萌发种子的α-淀粉酶和ATP酶活性及根尖细胞的有丝分裂,提高吲哚乙酸氧化酶活性,使种子萌发率和萌发指数及幼苗根长、株高和干重下降,这种抑制效应随水浸液浓度提高而增强,并与多年生黑麦草的种植密度呈负相关。在多年生黑麦草植株生长过程中浇灌多裂骆驼蓬水浸液提高了其叶片的超氧阴离子(O₂^·-)、H₂O₂和丙二醛(MDA)含量;超氧化物歧化酶(SOD)、过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)活性及抗坏血酸(ASA)含量随着水浸液浓度提高先升后降,过氧化氢酶(CAT)活性升高,谷胱甘肽(GSH)含量及光合速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr)降低,胞间CO₂浓度(Ci)升高。叶绿素荧光动力学资料显示,叶片初始荧光(Fo)随多裂骆驼蓬水浸液浓度提高而增加,最大荧光(Fm)、PSⅡ潜在光化学效率(Fv/Fo)、最大光化学效率(Fv/Fm)、光合电子传递量子效率(ΦPSⅡ)和光化学狡灭系数(qP)降低,非光化学狡灭系数(NPQ)先升后降,表明叶片光合机构受到伤害。

关键词: 多裂骆驼蓬, 多年生黑麦草, 化感作用, 光合作用, 叶绿素荧光参数

Abstract: In order to investigate the allelopathy of Peganum multisectum(Maxim.) Bobr.on perennial ryegrass(Lolium perenne L.),the effects of aqueous extract in different concentrations from P.multisectum were studied on the seed germination,seedling growth,and physiological-biochemical manifestation of perennial ryegrass using solution and pot-soil culture methods.The results show that the aqueous extract from P.multisectum significantly inhibited the activities of α-amylase and ATPase during seed germination and the mitosis in the meristem tissue of root tips but increased IAAoxidase activity of perennial ryegrass,and decreased the seed germination rate,germination index,root length,aboveground height,and dry weight;those inhibitory effects were enhanced along with the increased concentration of aqueous extract and negatively correlative with perennial ryegrass planting densities.Watering perennial ryegrass seedlings with the aqueous extract of P.multisectum increased the contents of O₂^·-,H₂O₂,and malondialdehy-de (MDA),catelase(CAT) activities,and intercellular carbon dioxide concentration of perennial ryegrassleaves,decreased glutathion(GSH) contents,net photosynthetic rate,stomatal conductance,and transpiration rate;the activities of superoxide dismutase(SOD),peroxidase(POD) and ascorbate peroxidase(APX) and ascorbic acid(ASA) content increased initially and then decreased with the increasing of aqueous extract concentration.The kinetic data of chlorophyll fluorescence revealed that,when the aqueous extract concentration increased,the minimal fluorescence(Fo) of perennial ryegrass leaves increased,the maximum fluorescence(Fm),PSⅡ potential fluorescence efficiency(Fv/Fo),PSⅡ maximum fluorescence efficiency(Fv/Fm),the actual photochemical efficiency of PSⅡ(ΦPSⅡ),and photochemical quenching(qP) decreased,and the non-photo chemical quenching(NPQ) increased at the beginning and then decreased,which indicated the leaf photosynthetic system was injured.

Key words: Peganum multisectum(Maxim.) Bobr., Perennial ryegrass, Allelopathy, Photosynthesis, Chlorophyll fluorescence parameter

中图分类号:

Q946

刘建新, 胡浩斌, 王鑫. 多裂骆驼蓬水浸液对多年生黑麦草的化感作用与生理生化表现[J]. , 2008, 16(4): 374-379.

LIU Jian-xin, HU Hao-bin, WANG Xin. Study on Allelopathy of Aqueous Extract from Peganum multisectum (Maxim.) Bobr. on Perennial Ryegrass (Lolium perenne L.) and Its Physiological-biochemical Manifestation[J]. , 2008, 16(4): 374-379.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2008.04.011

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2008/V16/I4/374

参考文献

[1] 孔垂华,胡飞.植物化感(相生相克)作用及其应用[M].北京:中国农业出版社,2001.202-226
[2] 马骥,王勋陵.中国荒漠地区骆驼蓬属植物种类与分布[J].中国沙漠,1998,18(2):131-136
[3] 邬彩霞,李志华,沈益新.豆科牧草水浸提液的酚酸物质含量及化感潜力[J].草地学报,2007,15(5):401-406
[4] Turk M A,Tawaha A M.Allelopathic effect of black mustard (Brassicanigra L.) on germination and growth of wild oat (Avena fatua L.)[J].Crop Prot.,2003,22:673-677
[5] 寇建村,杨文权,冯桂丽,等.不同苜蓿品种根、茎、叶水提液化感作用研究[J].草地学报,2008,16(1):70-75
[6] 刘建新,赵国林.多裂骆驼蓬对食荚豌豆的化感作用研究[J].中国生态农业学报,2007,15(1):12-15
[7] 刘建新,胡浩斌,雷蕊霞.多裂骆驼蓬对紫花苜蓿化感作用的生理生化机理[J].2007,29(4):39-44
[8] 耿广东,程智慧,孟焕文,等.西瓜化感作用及其机理研究[J].果树学报,2005,22(3):247-251
[9] 张志良,瞿伟菁.植物生理学实验指导(第三版)[M].北京:高等教育出版社,2003.39-262
[10] Mukherjee S P,Choudhuri M A.Implications of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings[J].Physiol.Plant,1983,58:166-174
[11] 陈建勋,王晓峰.植物生理学实验指导[M].广州:华南理工大学出版社,2002.119-129
[12] 张守仁.叶绿素荧光参数及其意义讨论[J].植物学通报,1999,16(4):444-448
[13] Nilsson M C.Separation of allelopathy and resource competition by the boroal dwarf shrub:Empetrum hermaphrodium[J].Hagerup Oecologia,1998:1-7
[14] 杨石先,陈茹玉,武振亮,等.骆驼蓬中活性物质的研究[J].植物生理学通讯,1987,(1):18-21
[15] Penuelas J,Ribas-Carbo M,Giles L.Effects of allelochemicals on plant respiration and oxygen isotope fractionation by the alternative oxidase[J].J.Chem.Ecol.,1996,22(4):801-805
[16] Baziramakenga R,Simard R R,Leroux G D.Effects of benzoic and cinnamic acids on growth,mineral composition,and chlorophyll content of soybean[J].J.Chem.Ecol.,1994,20(11):2821-2833
[17] Thomaszewiski M,Thimann K V.Interaction of phemolic acids,metabllicions and chlating agents on auxin-induced growth[J].Plant Physiol.,1966,41:1443-1454
[18] Einbhlling F A,Rasmussen J A,Schon M K.Effects of three phenolic acids on chlorophyll content and growth of soybean and grain sorghum seedlings[J].J.Chem.Ecol.,1979,4:425-436
[19] 郁继华,张韵,牛彩霞,等.两种化感物质对茄子幼苗光合作用及叶绿素荧光参数的影响[J].应用生态学报,2006,17(9):1629-1632
[20] Farquhar G D,Sharkey T D.Stomatal conductance and photosynthesis[J].Annual Review of Plant Physiology,1982,33:317-345
[21] Schnettger B,Critchley C,Santore U J,et al.Relationship between photoinhibition of photosynthesis,D1 protein turnover and cholroplast structure:effects of protein synthesis inhibitors[J].Plant Cell Environment,1994,17(1):55-64
[22] Krause G H,Weis E.Chlorophyll fluorescence and photosynthesis:The basics[J].Annu.Rev.Plant Physiol.Plant Mol.Biol.,1991,42:313-349
[23] Griffin J J,Ranney T G,Pharr D M.Photosynthesis,chlorophyll fluorescence,and carbohydrate content of Illicium taxa grown under varied irradiance[J].Journal of the America Society for Horticultural Science,2004,129(1):46-53
[24] 姜述君,强胜.马唐生防菌画眉草弯孢霉毒素α,β-dehydrocurvularin对马唐叶片PSⅡ功能的影响[J].中国农业科学,2005,38(7):1373-1378

多裂骆驼蓬水浸液对多年生黑麦草的化感作用与生理生化表现

Study on Allelopathy of Aqueous Extract from Peganum multisectum (Maxim.) Bobr. on Perennial Ryegrass (Lolium perenne L.) and Its Physiological-biochemical Manifestation

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	左郎, 王树森, 马迎梅, 温苏雅勒图, 白刚, 郁鑫明. 火炬树浸提液对两种草坪草种子萌发的影响[J]. 草地学报, 2021, 29(9): 1927-1933.
[2]	王宝强, 赵颖, 朱晓林, 王旺田, 魏小红. 盐碱胁迫对藜麦幼苗叶片光合特性及抗氧化系统的影响[J]. 草地学报, 2021, 29(8): 1689-1696.
[3]	马银山, 常妍, 王含睿, 贾云凤, 李文英, 漆瑞强. 天仙子种子浸提液对黑麦草种子萌发和幼苗生长的化感作用[J]. 草地学报, 2021, 29(8): 1697-1703.
[4]	张新飞, 佘木子, 李晗玉, 敬松, 姜惠娜, 高浩, 朱延晓, 付娟娟. 琥珀酸黄杆菌促生机理及其对多年生黑麦草生长和抗逆性的生理调控作用[J]. 草地学报, 2021, 29(8): 1704-1711.
[5]	张金花, 何静, 张小彦, 张树衡, 张恒, 李彦湘. 除草剂胁迫下3种牧草浸提液对受体植物种子萌发和幼苗生长的影响[J]. 草地学报, 2021, 29(8): 1719-1728.
[6]	李善魁, 孟焕文, 李宇晨, 张海青, 白雪, 张宇宽, 周洪友, 王千军, 张笑宇. 圆柏对几种农田杂草及作物的化感潜力分析[J]. 草地学报, 2021, 29(8): 1769-1778.
[7]	秦金萍, 刘颖, 马玉寿, 王彦龙, 李世雄. 返青期休牧对退化高寒草地植被特征及优势种光合生理的影响[J]. 草地学报, 2021, 29(5): 1034-1042.
[8]	段梦琪, 彭春, 张睿, 高娅楠, 李丽菁, 张智韦, 许立新. 乙烯利对干旱条件下多年生黑麦草生理指标的影响[J]. 草地学报, 2021, 29(2): 333-341.
[9]	赵利清, 彭向永, 刘俊祥, 毛金梅, 孙振元. GSH对铅胁迫下多年生黑麦草幼苗抗氧化系统的调控[J]. 草地学报, 2020, 28(6): 1527-1534.
[10]	尉欣荣, 张智伟, 周雨, 刘鹏, 姚宏斌, 苗彦军, 付娟娟. 褪黑素对低温和干旱胁迫下多年生黑麦草幼苗生长和抗氧化系统的调节作用[J]. 草地学报, 2020, 28(5): 1337-1345.
[11]	王军, 赵桂琴, 柴继宽, 王苗苗, 贾雪洁, 郭志飞, 孙雷雷, 聂秀美. 大麦黄矮病毒侵染对燕麦光合及叶绿素荧光参数的影响[J]. 草地学报, 2020, 28(4): 923-931.
[12]	李双铭, 罗建章, 陈才发, 向佐湘, 胡龙兴. 浦绿对多年生黑麦草耐热性及坪用性能的影响[J]. 草地学报, 2020, 28(4): 1006-1014.
[13]	赖帅彬, 潘新雅, 简春霞, 李军保, 郭尚洙, 徐炳成, 王智. 转苜蓿MsOr基因烟草光合-光响应和光合-CO₂响应曲线特征研究[J]. 草地学报, 2020, 28(1): 20-30.
[14]	梁欢, 韦宝, 陈静, 宫文龙, 马琳, 王学敏, 王赞. 基于叶绿素荧光参数的紫花苜蓿种质苗期抗旱性评价[J]. 草地学报, 2020, 28(1): 45-55.
[15]	罗艺岚, 赵东豪, 孙鹏越, 高金柱, 呼天明, 付娟娟. 遮阴胁迫对麦冬和多年生黑麦草根际土壤细菌群落结构和多样性的影响[J]. 草地学报, 2019, 27(5): 1204-1212.