干旱胁迫下根瘤菌共生紫花苜蓿抗旱生理变化研究

doi:10.11733/j.issn.1007-0435.2013.05.016

草地学报 ›› 2013, Vol. 21 ›› Issue (5): 938-944.DOI: 10.11733/j.issn.1007-0435.2013.05.016

干旱胁迫下根瘤菌共生紫花苜蓿抗旱生理变化研究

张攀, 杨培志, 王卫栋, 张志强, 韩博, 王亚芳, 曹玉曼, 呼天明

西北农林科技大学动物科技学院草业科学系, 陕西杨凌 712100

收稿日期:2013-03-11 修回日期:2013-05-19 出版日期:2013-10-15 发布日期:2013-10-30
通讯作者: 呼天明
作者简介:张攀(1984- ),男,安徽亳州人,博士研究生,主要从事牧草遗传资源与利用研究,E-mail:zhangpgarss@126.com
基金资助:
国家自然科学基金项目(30901050)(31272490);国家"十二五"支撑计划(2011BAD17B05)资助

Study on Physiological Change of Alfalfa with Symbiotic Rhizobium under Drought Stress

ZHANG Pan, YANG Pei-zhi, WANG Wei-dong, ZHANG Zhi-qiang, HAN Bo, WANG Ya-fang, CAO Yu-man, HU Tian-ming

Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi Provnice 712100, China

Received:2013-03-11 Revised:2013-05-19 Online:2013-10-15 Published:2013-10-30

摘要/Abstract

摘要： 为研究根瘤菌共生对干旱胁迫下紫花苜蓿(Medicago sativa L.)抗旱生理指标变化的影响,对空气干旱条件下接种根瘤菌和不接种根瘤菌的紫花苜蓿的叶片相对含水量及根、茎、叶中丙二醛(MDA)、脯氨酸(Pro)、可溶性糖、还原性谷胱甘肽的(GSH)含量及超氧化物歧化酶(SOD)和过氧化物酶(POD)活性的变化进行测定。结果表明:干旱胁迫下,根瘤菌共生紫花苜蓿叶片相对含水量在干旱前期显著高于不接种根瘤菌紫花苜蓿;不接种根瘤菌紫花苜蓿根、茎、叶中MDA含量明显高于根瘤菌共生紫花苜蓿,各胁迫时间点均差异显著(P<0.05)。根瘤菌共生紫花苜蓿根、茎、叶中可溶性糖含量、Pro含量、GSH含量及SOD 和POD活性在干旱后期明显高于不接种根瘤菌紫花苜蓿(P<0.05)。根瘤菌共生紫花苜蓿具有更强的渗透调节能力和活性氧防御能力。

关键词: 紫花苜蓿, 根瘤菌, 共生, 干旱胁迫, 生理指标

Abstract: The leaf relative water, malondialdehyde (MDA), proline (Pro), soluble sugar and reduced glutathione (GSH) contents, as well as the superoxide dismutase (SOD) and peroxiase (POD) activities of alfalfa inoculated rhizobium and not-inoculated rhizobium were studied in order to investigate the physiological change of alfalfa with symbiotic rhizobium under drought stress. Results showed that the leaf water content of alfalfa inoculated rhizobium was significantly higher than not-inoculated alfalfa under drought stress. The MDA content of alfalfa inoculated rhizobium was significantly higher than not-inoculated alfalfa under drought stress. The Pro content, soluble sugar content, GSH content, SOD and POD activities of inoculated alfalfa had significant difference compared with that of not-inoculated alfalfa under drought stress. Both osmotic adjustment ability and reactive oxygen defense capability of alfalfa inoculated rhizobium were much better than not-inoculated alfalfa.

Key words: Alfalfa, Rhizobium, Symbiotic, Drought stress, Physiological parameters

中图分类号:

张攀, 杨培志, 王卫栋, 张志强, 韩博, 王亚芳, 曹玉曼, 呼天明. 干旱胁迫下根瘤菌共生紫花苜蓿抗旱生理变化研究[J]. 草地学报, 2013, 21(5): 938-944.

ZHANG Pan, YANG Pei-zhi, WANG Wei-dong, ZHANG Zhi-qiang, HAN Bo, WANG Ya-fang, CAO Yu-man, HU Tian-ming. Study on Physiological Change of Alfalfa with Symbiotic Rhizobium under Drought Stress[J]. Acta Agrestia Sinica, 2013, 21(5): 938-944.

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干旱胁迫下根瘤菌共生紫花苜蓿抗旱生理变化研究

Study on Physiological Change of Alfalfa with Symbiotic Rhizobium under Drought Stress

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