草地学报

草地学报 ›› 2015, Vol. 23 ›› Issue (3): 661-664.DOI: 10.11733/j.issn.1007-0435.2015.03.033

• 研究专报 • 上一篇    

高寒草地土壤5株真菌合成不同功能酶系间互作效应的研究

芦光新1, 温小成1, 王军邦2, 吴楚3, 陈秀蓉4   

  1. 1. 青海大学农牧学院, 青海 西宁 810016;
    2. 中国科学院地理科学与资源研究所, 北京 100094;
    3. 长江大学园艺园林学院, 湖北 荆州 434025;
    4. 甘肃农业大学草业学院, 甘肃 兰州 730070
  • 收稿日期:2014-04-23 修回日期:2014-09-22 出版日期:2015-06-15 发布日期:2015-07-02
  • 通讯作者: 王军邦, 陈秀蓉
  • 作者简介:芦光新(1974-),男,青海湟中人,博士,教授,研究方向为高寒草地土壤微生物多样性及功能利用,E-mail:lugx74@qq.com
  • 基金资助:

    国家自然科学基金“青藏高原草地耐低温纤维素分解真菌多样性研究”(41261064)(31460152);青海省科学技术厅项目“小皮伞属(Marasmius tricolor)真菌产漆酶特性及有机污染物处理技术研究”(2014-ZJ-924);青海大学高层次人才基金“产漆酶真菌的筛选及多酶系菌群的构建”(2012-QGC-9);“中国新西兰高原草地营养流及可持续生产研究”(2015DFG31870)资助

Interactions among Different Enzymes of Five Fungal Strains from Alpine Meadow Soil

LU Guang-xin1, WEN Xiao-cheng1, WANG Jun-bang2, WU Chu3, CHEN Xiu-rong4   

  1. 1. Agriculture and Animal husbandry College, Qinghai University, Xining, Qinghai Province 810016, China;
    2. Institute of Geographic Sciences and Nature Resources Research, CAS, Beijing 100094, China;
    3. College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei Province 434025, China;
    4. Pratacultural College, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China
  • Received:2014-04-23 Revised:2014-09-22 Online:2015-06-15 Published:2015-07-02

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摘要：

为研究筛选自高寒草地5株真菌合成不同功能酶系间的互作效应,采用单个菌株发酵粗酶液直接混合和菌株组合发酵的2种方式,分别测定了反应体系中不同功能酶活力的变化情况。结果表明:菌株310b与4个菌株23a,26B,24d及H发酵粗酶液直接混合的情况下,反应体系中漆酶、羧甲基纤维素酶、木聚糖酶的酶活力下降, 且差异显著(P<0.05);而菌株310b与4个菌株组合发酵的情况下,反应体系中漆酶的酶活力下降,且差异显著(P<0.05),在与菌株23a和H的组合中,反应体系羧甲基纤维素酶和木聚糖酶的酶活力下降,且差异显著(P<0.05),与菌株26B和24d 的组合中,反应体系中羧甲基纤维素酶和木聚糖酶的酶活力没有变化。

关键词: 高寒草地土壤, 真菌, 不同功能酶系, 互作效应

Abstract:

It is very significant to study the interactions between cellulolytic enzymes because the interactions could increase the decomposition of cellulose. In the paper, five fungal strains from alpine meadow soil were selected to compose a synthetic system. The five fungal strains had the high activities of cellulolytic enzymes. The activities of cellulolytic enzymes of the individual fungal strain or the different mixed fungal strains were determined and the interactions between cellulolytic enzymes in the fungal strains were analyzed. The results showed that compared with the individual strains, the activities of cellulases, Hemicellulase, and laccases in the mixed solutions which were composed of the same volume of enzymes from the two of the five fungal strains decreased(P<0.05), and the decreases of these enzyme activities were different. The celluloses and hemicelluloses activities of the mixed enzymes from the 310b and 23a strains as well as from the 310b and H strains changed significantly(P<0.05), whereas there were not significant changes in the mixed enzymes of 310b and 24d strains as well as in the mixed enzymes of 310b and 26B strains.

Key words: Alpine meadow soil, Fungal strains, Different functional enzymes, Interaction effect

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