草地学报

草地学报 ›› 2015, Vol. 23 ›› Issue (3): 657-660.DOI: 10.11733/j.issn.1007-0435.2015.03.032

• 研究专报 • 上一篇    下一篇

油菜素内酯对石油污染下紫穗槐苗木抗氧化系统的影响

韩园园1, 韩刚2, 李凯荣3   

  1. 1. 西北农林科技大学水土保持研究所, 陕西 杨凌 712100;
    2. 西北农林科技大学林学院, 陕西 杨凌 712100;
    3. 西北农林科技大学资源环境学院, 陕西 杨凌 712100
  • 收稿日期:2014-08-04 修回日期:2014-12-23 出版日期:2015-06-15 发布日期:2015-07-02
  • 通讯作者: 李凯荣
  • 作者简介:韩园园(1989-),女,陕西扶风人,硕士生,主要从事林业生态工程研究,E-mail:767253448@163.com
  • 基金资助:

    林业公益性行业科研专项课题—油田污染胁迫立地植被恢复技术研究(201104002-4);西北农林科技大学基本科研业务费专项—陕北主要造林树种对石油污染的耐性研究(QN2011162)资助

Effect of Brassinolide (BR) on the Antioxidant System of Amorpha fruticosa Seedlings under Petroleum Pollution

HAN Yuan-yuan1, HAN Gang2, LI Kai-rong3   

  1. 1. Institute of Soil and Water Conservation, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
    2. College of Forestry, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
    3. College of Resource and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100
  • Received:2014-08-04 Revised:2014-12-23 Online:2015-06-15 Published:2015-07-02

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摘要：

为探讨油菜素内酯(BR)对石油污染下紫穗槐(Amorpha fruticosa)苗木抗氧化系统的影响,进行室内盆栽试验,用不同浓度油菜素内酯(BR)对苗木蘸根和叶面喷施后,测定其抗氧化酶活性和抗氧化剂含量。结果表明:BR能显著提高超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)和谷胱甘肽还原酶(GR)的活性,显著增加抗坏血酸(ASA)和还原型谷胱甘肽(GSH)含量,增强紫穗槐抗石油污染性,而且以0.3 mg·L-1的油菜素内酯浓度效果最佳。

关键词: 紫穗槐, 油菜素内酯, 土壤石油污染, 抗氧化系统

Abstract:

To explore the effect of brassinolide(BR) on the antioxidant system of Amorpha fruticosa seedlings under petroleum pollution, the indoor potted experiment was used by dipping roots and spraying leaves of seedlings with different concentrations of brassinolide. The results showed that brassinolide significantly increased the antioxidant protection enzyme activities of SOD, APX, CAT and GR, the contents of ASA and GSH that kept the active oxygen content of seedlings in balance to avoid oxidative damage to cells. The most significant concentration of brassinolide was 0.3 mg·L-1 for Amorpha fruticosa.

Key words: Amorpha fruticosa, Brassinolide, Soil petroleum pollution, Antioxidant system

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