外源油菜素内酯引发对劣变燕麦种子抗氧化特性的影响

doi:10.11733/j.issn.1007-0435.2023.05.021

草地学报 ›› 2023, Vol. 31 ›› Issue (5): 1461-1468.DOI: 10.11733/j.issn.1007-0435.2023.05.021

外源油菜素内酯引发对劣变燕麦种子抗氧化特性的影响

王勃, 夏方山, 董秋丽, 岑慧芳, 李尹琳, 张杰敏

山西农业大学草业学院, 山西太谷 030801

收稿日期:2022-11-11 修回日期:2023-01-01 出版日期:2023-05-15 发布日期:2023-05-31
通讯作者: 夏方山,E-mail:dqlxfs8583@163.com
作者简介:王勃(1998-)，女，汉，山西晋城人，硕士研究生，主要从事草种子科学研究，E-mail:wangbo10898@163.com
基金资助:
山西农业大学“十四五”生物育种工程项目(YZGC134)；山西省重点研发计划项目(202102140601006，201903D221091)；内蒙古大学“省部共建草原家畜生殖调控与繁育”国家重点实验室开放课题(2021KF0303)资助

Effect of Exogenous Brassinosteroids Priming on Antioxidant Characteristics of Deteriorated Oat Seeds

WANG Bo, XIA Fang-shan, DONG Qiu-li, CEN Hui-fang, LI Yin-lin, ZHANG Jie-min

College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China

Received:2022-11-11 Revised:2023-01-01 Online:2023-05-15 Published:2023-05-31

摘要/Abstract

摘要： 为探讨外源油菜素内酯引发对劣变种子抗氧化能力的影响,试验采用燕麦(Avena sativa)种子为材料,人工老化后经不同浓度(0,0.01,0.10,0.50,1.00,10.00 μmol·L^-1)的油菜素内酯(Brassinosteroids,BR)溶液引发0(CK),1,6,12,18和24 h,分析了其发芽率、超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)、抗坏血酸过氧化物酶(Ascorbate peroxidase,APX)、谷胱甘肽还原酶(glutathione reductase,GR)活性及丙二醛(Maloddialdehyde,MDA)含量的变化规律。结果表明:低浓度(0.01~0.50 μmol·L^-1)的BR可提高燕麦种子的发芽率,增加其SOD,CAT,APX和GR活性,降低其MDA含量;而BR浓度为10.00 μmol·L^-1时(除引发18 h外),其发芽率、CAT、APX和GR活性均低于CK,增加MDA含量。浓度为0.01 μmol·L^-1的油菜素内酯引发24 h可能是缓解燕麦种子老化的最佳处理。

关键词: 油菜素内酯, 燕麦, 种子老化, 种子引发, 抗氧化性能

Abstract: In order to explore the effect of exogenous brassinosteroid priming on the antioxidant capacity of deteriorated seeds,Oat (Avena sativa) seeds were used as materials,and tested them with different concentrations (0,0.01,0.10,0.50,1.00,10.00 μmol·L^-1) brassinosteroid solution initiated for 0 (CK),1,6,12,18 and 24 h durations. The changes of germination rate,superoxide dismutase (SOD),catalase (CAT),ascorbate peroxidase (APX),glutathione reductase (GR) and malondialdehyde (MDA) content were analyzed. The results showed that low concentration (0.01~0.50 μmol·L^-1) of BR improved the germination rate of oat seeds,and increase the activities of SOD,CAT,APX and GR,and reduce the content of MDA in the oat seeds. When the concentration of BR reached 10.00 μmol·L^-1 (except for 18 h initiation),the germination rate,CAT,APX and GR activities were lower than those of the seeds in CK,and the MDA content was higher. Priming with 0.01 μmol·L^-1 brassinolide for 24 h may be the best treatment to alleviate the aging of oat seeds.

Key words: Brassinosteroids, Oats, Seed aging, Seed priming, Oxidation resistance

中图分类号:

S152.6

王勃, 夏方山, 董秋丽, 岑慧芳, 李尹琳, 张杰敏. 外源油菜素内酯引发对劣变燕麦种子抗氧化特性的影响[J]. 草地学报, 2023, 31(5): 1461-1468.

WANG Bo, XIA Fang-shan, DONG Qiu-li, CEN Hui-fang, LI Yin-lin, ZHANG Jie-min. Effect of Exogenous Brassinosteroids Priming on Antioxidant Characteristics of Deteriorated Oat Seeds[J]. Acta Agrestia Sinica, 2023, 31(5): 1461-1468.

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https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2023/V31/I5/1461

参考文献

[1] 南志标,王彦荣,贺金生,等. 我国草种业的成就、挑战与展望[J]. 草业学报,2022,31(6):1-10
[2] 刘加文. 大力发展中国草种业. 草地学报,2016,24(3):483-484
[3] WANG B,YANG R C,JI Z Q,et al. Evaluation of Biochemical and Physiological Changes in Sweet Corn Seeds under Natural Aging and Artificial Accelerated Aging[J]. Agronomy,2022,12(5):1028
[4] MICHALAK M,PLITTA-MICHALAK B P,NASKReTBARCISZEWSKA M Z,et al. DNA Methylation as an Early Indicator of Aging in Stored Seeds of "Exceptional" Species Populus nigra L.[J]. Cells,2022,11(13):2080
[5] LIN Y X,XU H J,YIN G K,et al. Dynamic Changes in Membrane Lipid Metabolism and Antioxidant Defense During Soybean (Glycine max L. Merr.) Seed Aging[J]. Frontiers in Plant Science,2022,13:908949
[6] 夏方山,董秋丽,毛培胜,等. PEG引发对老化燕麦种胚细胞与线粒体结构及抗氧化性能的影响[J]. 草业学报,2018,27(5):170-177
[7] 王勃,王聪聪,夏方山,等. 硒引发对不同品种紫花苜蓿种子抗氧化特性的影响[J]. 草地学报,2022,30(8):2037-2044
[8] 雷新慧,万晨茜,陶金才,等. 褪黑素与2,4-表油菜素内酯浸种对盐胁迫下荞麦发芽与幼苗生长的促进效应[J]. 作物学报,2022,48(5):1210-1221
[9] 丁美云,刘丽杰,于梦迪,等.油菜素内酯对寒地冬小麦越冬期生理代谢的影响[J]. 麦类作物学报,2022(7):1-8
[10] XU Q S,WEI Q Q,KONG Y L,et al. Unearthing the Alleviatory Mechanisms of Brassinolide in Cold Stress in Rice[J]. Life,2022,12(6):833
[11] 朱瑞婷,牛奎举,张娣君,等. 外源2,4-表油菜素内酯对镉胁迫下草地早熟禾叶绿素代谢的影响[J]. 草地学报,2021,29(4):635-643
[12] TANG W J,XIAO L,PENG X M,et al. Effects of Brassinolide on Cadmium Accumulation and Growth of Emerged Accumulator Plant;Nasturtium Officinale[J]. Chemistry and Ecology,2022,38(4):301-311
[13] LUKATKIN A S,KASHTANOVA N N,DUCHOVSKIS P. Changes in Maize Seedlings Growth and Membrane Permeability under the Effect of Epibrassinolide and Heavy Metals[J]. Russian Agricultural Sciences,2013,39(4):307-310
[14] LI M H,ZHANG Y F,XU X H, et al. The Combined Treatments of Brassinolide and Zeaxanthin Better Alleviate Oxidative Damage and Improve Hypocotyl Length,Biomass,and the Quality of Radish Sprouts Stored at Low Temperature[J]. Food Chemistry:X,2022(15):100394
[15] SUDARSHNA K,ANJU T. Morphological and Physio-biochemical Changes in Response to Foliar Application of Brassinosteroid and Water Stress in Apple Plants Under Pot Culture Study[J]. International Journal of Bio-resource and Stress Management,2019,10(1):39-45
[16] 梁栋. IAA和BR参与干旱胁迫影响烟草侧根发育的研究[D]. 北京:中国农业科学院,2021:39-41
[17] KHAN R,MA X H,HUSSAIN Q,et al. Application of 2,4-Epibrassinolide Improves Drought Tolerance in Tobacco through Physiological and Biochemical Mechanisms[J]. Biology,2022,11(8):1192
[18] MOTALLEB H,ALI E,HASSAN H,et al. Enhancing Enzymatic and Nonenzymatic Response of Echinacea Purpurea by Exogenous 24-Epibrassinolide under Drought Stress[J]. Industrial Crops and Products,2020,146(C):112045
[19] 权梦萍,徐佳慧,尹佳茗,等. 油菜素内酯调控植物响应非生物逆境胁迫的生理机制[J].2023,50(1):22-31
[20] KAYA C,ASHRAF M,ALYEMENI M N,et al. The Role of Nitrate Reductase in Brassinosteroid-induced Endogenous Nitric Oxide Generation to Improve Cadmium Stress Tolerance of Pepper Plants by Upregulating the Ascorbate-glutathione Cycle[J]. Ecotoxicology and Environmental Safety,2020,196(C):110483
[21] 黄颖,刘欢,赵桂琴,等. 自然老化和人工老化对燕麦种子萌发特性及遗传完整性的影响[J]. 草地学报,2022,30(8):2066-2074
[22] 夏方山. 不同老化处理对燕麦种子线粒体结构及抗氧化系统的影响[D]. 北京:中国农业大学,2015:15-16
[23] 金小雯. 贮藏年限对燕麦种子生理生化特性的影响[D]. 兰州:甘肃农业大学,2019:16-18
[24] 朱连发,罗兴雨,柏晓玲,等. NaCl和KNO₃浸种对老化燕麦种子萌发的影响[J]. 草学,2021(3):28-31,54
[25] YAN H F,MAO P S. Comparative Time-Course Physiological Responses and Proteomic Analysis of Melatonin Priming on Promoting Germination in Aged Oat (Avena sativa L.) Seeds[J]. International Journal of Molecular Sciences,2021,22(2):811
[26] MAO C L,ZHU Y Q,CHENG H,et al. Nitric Oxide Regulates Seedling Growth and Mitochondrial Responses in Aged Oat Seeds[J]. International Journal of Molecular Sciences,2018,19(4):1502
[27] 刘备,宋玉梅,孙铭,等. 燕麦劣变种子吸胀过程中线粒体AsA-GSH循环的生理响应[J]. 草地学报,2021,29(2):211-219
[28] ISTA. International Rules for Seed Testing. International Seed Testing Association[S]. Bassersdorf:Switzerland,2019
[29] KIBINZA S,VINEL D,COME D,et al. Sunflower Seed Deterioration as Related to Moisture Content during Ageing,Energy Metaboli Sm and Active Oxygen Species Scavenging[J]. Physiologia Plantarum,2006(128):496-506
[30] RAO K V M,SRESTY T V S. Antioxidant Parameters in the Seedlings of Pigeon Pea (Cajanus cajan (L.) Millspaugh) in Response to Zn and Ni Stresses[J]. Plant Science,2000(157):113-128
[31] AEBI H E. Catalase. In Methods of Enzymatic Analysis[M]. Verlag Chemie,Weinheim,1986:273-286
[32] NAKANO Y,ASADA K. Hydrogen Peroxide Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplast[J]. Plant and Cell Physiology,1981(22):867-880
[33] MADAMANCHI N R,ALSCHER R G. Metabolic Bases for Differences in Sensitivity of Two Pea Cultivars to Sulfur Dioxide[J]. Plant Physiology,1991,97(1):88-93
[34] BAILLY C,BENAMAR A,CORBINEAU F,et al. Changes in Malondialdehyde Content and Superoxide Dismutase,Catalase and Glutathione Reductase Activities in Sunflower Seeds as Related to Deterioration during Accelerated Aging[J]. Physiologia Plantarum,1996(97):104-111
[35] 范翠枝,吴馨怡,关欣,等. 油菜素内酯浸种对盐胁迫番茄种子萌发的影响及其生理机制[J]. 生态学报,2021,41(5):1857-1867
[36] 王丹,刘亚西,周扬,等. 油菜素内酯对盐胁迫下黑麦草种子萌发及幼苗生长的生理调控作用[J]. 草业科学,2021,38(6):1110-1118
[37] 隋欣. 油菜素内酯对干旱胁迫下紫花苜蓿种子萌发、生理响应和生产性能的影响[D]. 咸阳:西北农林科技大学,2022:34-35
[38] 李红玉,王雅聪,曾佳,等. 外源H₂O₂引发对燕麦种胚细胞AsA-GSH循环的影响[J].草地学报,2022,30(7):1668-1674
[39] 石欣隆,薛娴,杨月琴,等. 2,4-表油菜素内酯对低温胁迫下花生幼苗生长及生理特性的影响[J]. 2023,45(2):341-348

外源油菜素内酯引发对劣变燕麦种子抗氧化特性的影响

Effect of Exogenous Brassinosteroids Priming on Antioxidant Characteristics of Deteriorated Oat Seeds

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