贝加尔针茅草原土壤线虫与微生物群落特征及其相互作用

doi:10.11733/j.issn.1007-0435.2018.01.009

草地学报 ›› 2018, Vol. 26 ›› Issue (1): 77-84.DOI: 10.11733/j.issn.1007-0435.2018.01.009

贝加尔针茅草原土壤线虫与微生物群落特征及其相互作用

张爱林¹, 赵建宁², 洪杰³, 杨殿林¹

1. 农业部环境保护科研监测所, 天津 300191;
2. 沈阳农业大学园艺学院, 辽宁沈阳 11086;
3. 内蒙古鄂温克旗草原工作站, 内蒙古巴彦托海 021100

收稿日期:2017-08-09 修回日期:2018-03-12 出版日期:2018-02-15 发布日期:2018-05-25
通讯作者: 杨殿林
作者简介:张爱林(1992-),女,山东临沂人,硕士研究生,主要从事草原生态与恢复研究,E-mail:zal18669553371@163.com
基金资助:
国家自然科学基金项目（31170435）资助

Characteristics and Interaction of Soil Nematodes and Microbial Communities in Stipa Grandis Grassland

ZHANG Ai-lin¹, ZHANG Jian-ning², HONG Jie³, YANG Dian-lin¹

1. Agro-environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China;
2. College of Horticulture, Shenyang Agriculture University, Shenyang, Liaoning Province 110866, China;
3. Inner Mongolia Ewenki Flag Grassland Workstation, BayanTorr, Inner Mongolia 021100, China

Received:2017-08-09 Revised:2018-03-12 Online:2018-02-15 Published:2018-05-25

摘要/Abstract

摘要：

分析了在施氮环境下内蒙古贝加尔针茅草原土壤微生物和土壤线虫群落特征及相互作用。结果表明，不同的施氮水平显著影响土壤微生物真菌和细菌群落结构和数量，但对土壤微生物群落总量的影响并不显著；对土壤线虫食细菌类群（Bacterivores）、食真菌类群（Fungivores）和植物寄生性类群（Plant parasites）数量影响显著，同时影响土壤线虫的物种丰富度（SR）和群落结构的稳定性。土壤中的植物寄生性类群线虫与土壤中细菌群落、真菌群落和真菌/细菌之间呈显著正相关，土壤真菌群落与土壤线虫群落的丰富度（SR）、均匀度（J'）和结构指数（WI）也有明显的相关性。施氮对土壤真菌群落的影响大于对土壤细菌群落的影响，土壤中的真菌群落与土壤中植物寄生性类群线虫具有相互促进的作用，增加了植物受线虫感染的几率，同时施氮也改变了土壤线虫的群落结构。

关键词: 贝加尔针茅草原, 施氮, 微生物群落, 土壤线虫

Abstract:

The characteristics and interactions of soil microbes and soil nematode communities under different levels of nitrogen application in Stipa sinensis grassland in Inner Mongolia were analyzed. As a result of the analysis, the nitrogen application significantly affected the fungal and bacterial community structure and quantity of soil microorganisms, but the effect on soil microbial community was not significant. It had a significant effect on the number of soil nematodes of bacterial groups(Bacterivores),fungal groups (Fungivores) and plant parasite groups. It also affected the species richness (SR) of the soil nematode and the stability of the community structure.There was a significant positive correlation between soil parasitic plant nematodes and soil bacterial communities, fungal communities, and fungi/bacteria.There was also a significant correlation between soil fungal community and soil nematode community richness (SR), evenness (J') and structural index (WI). The experimental results showed that the effect of nitrogen application on the soil fungal community was greater than that on the soil bacterial community. The fungal community in the soil and the plant parasitic group nematode in the soil have mutual promoting effects, which increased the infection rate of the plant by nematodes, and nitrogen application also changed the community structure of soil nematodes.

Key words: Stipa baicalensis steppe, Nitrogen application, Microbial community, Soil nematode

中图分类号:

S154.36

张爱林, 赵建宁, 洪杰, 杨殿林. 贝加尔针茅草原土壤线虫与微生物群落特征及其相互作用[J]. 草地学报, 2018, 26(1): 77-84.

ZHANG Ai-lin, ZHANG Jian-ning, HONG Jie, YANG Dian-lin. Characteristics and Interaction of Soil Nematodes and Microbial Communities in Stipa Grandis Grassland[J]. Acta Agrestia Sinica, 2018, 26(1): 77-84.

参考文献

[1] Bai H H,Bao X L,Sun XM,et al. The Effect of Stocking Rate on Soil Glomalin under Traditional and Mixed Grazing Systems in a Temperate Steppe[J]. Procedia Environmental Sciences,2011,11:817-823
[2] Lin H L,Feng Q S,Liang T G,et al. Modelling global-scale potential grassland changes in spatio-temporal patterns to global climate change[J]. International Journal of Sustainable Development and World Ecology,2013,1:83-96
[3] Lee M,Manning P,Rist J,et al. A global comparison of grassland biomass responses to CO2 and nitrogen enrichment[J]. Philosophical Transactions of the Royal Society B-Biological Sciences,2010,365(1549):2047-2056
[4] 潘庆民,白永飞,韩兴国,等. 氮素对内蒙古典型草原羊草种群的影响[J]. 植物生态学报, 2005,29(02):311-317
[5] 白春利,阿拉塔,陈海军,等. 氮素和水分添加对短花针茅荒漠草原植物群落特征的影响[J]. 中国草地学报,2013,35(02):69-75
[6] McCulley R L,Burke I C,Naison J A,et al. Regional Patterns in Carbon Cycling Across the Great Plains of North America[J]. Ecosystems,2005,8(1):106-121
[7] Sutherland W J,Amstrong-Brown S,Armsworth P R,et al. The identification of 100 ecological questions of high policy relevance in the UK[J]. Journal of applied ecology,2006,43(4):617-627
[8] 杨殿林,韩国栋,胡跃高,等. 放牧对贝加尔针茅草原群落植物多样性和生产力的影响[J].生态学杂志,2006,25(12):1470-1475
[9] Clark C M,Tilman D. Loss of plant species after chronic low-level nitrogen deposition to prairie grasslands[J]. Nature,2008,451(7179):712-715
[10] Carly J S,Nancy B D,David J G,et al. Impact of Nitrogen Deposition on the Species Richness of Grasslands[J]. Science,2004,303(5665):1876-1879
[11] 毛小芳,李辉信,陈小云,等. 土壤线虫三种分离方法效率比较[J]. 生态学杂志,2004,23(03):149-151
[12] Tom B,Marina B. Functional diversity of nematodes[J]. Applied soil ecology,1998,10(3):239-251
[13] David C D,Cecily A F,Kam T L,et al. In situ microbial ecology for quantitative appraisal, monitoring, and risk assessment of pollution remediation in soils, the subsurface, the rhizosphere and in biofilms[J]. Journal of Microbiological Methods,1998,32(2):93-105
[14] Kong A Y,Kate M S,William E H,et al. Microbial community composition and carbon cycling within soil microenvironments of conventional, low-input, and organic cropping systems[J]. Soil Biology&Biochemistry,2011,43(1):20-30
[15] Tunlid. Microbial biomass measured as total lipid phosphate in soils of different organic content[J]. Journal of Microbiological Methods,1991,14(3):151-163
[16] Fierer N,Joshua P S,Patricia A H. Variations in microbial community composition through two soil depth profiles[J]. Soil Biology&Biochemistry,2003,35(1):167-176
[17] Kulmatiski A,Karen H B. Long-term plant growth legacies overwhelm short-term plant growth effects on soil microbial community structure[J]. Soil Biology&Biochemistry, 2011,43(4):823-830
[18] 刘中良,宇万太,周桦,等. 长期施肥对土壤团聚体分布和养分含量的影响[J]. 土壤,2011,43(05):720-728
[19] 郭萍,文庭池,董玲玲,等. 施肥对土壤养分含量、微生物数量和酶活性的影响[J]. 农业现代化研究,2011(03):362-366
[20] 刘晓梅,方建,张婧,等. 长期施肥对麦田土壤微生物垂直分布的影响[J]. 植物生态学报, 2009(02):397-404
[21] Zhao S C,Qiu S J,Cao C Y,et al. Responses of soil properties, microbial community and crop yields to various rates of nitrogen fertilization in a wheat-maize cropping system in north-central China[J]. Agriculture, Ecosystems & Environment,2014,194:29-37
[22] Jiang Y J,Sun B,Chen J,et al. Soil aggregate stratification of nematodes and microbial communities affects the metabolic quotient in an acid soil[J]. Soil Biology&Biochemistry, 2013,60:1-9
[23] 郭永盛,李俊华,李鲁华,等. 施氮肥对荒漠草原土壤微生物种群及微生物量的影响[J]. 新疆农业科学,2011,48(01):79-85
[24] 施瑶,王忠强,张心昱,等. 氮磷添加对内蒙古温带典型草原土壤微生物群落结构的影响[J]. 生态学报,2014,34(17):4943-4949
[25] 高中超,迟凤琴,赵秋. 施肥对退化草原植物群落产量及土壤理化性质的影响[J]. 草原与草坪,2007(02):60-62
[26] Tu C,Koenning S R,Hu S. Root-Parasitic Nematodes Enhance Soil Microbial Activities and Nitrogen Mineralization[J]. Microbial Ecology,2003,46(1):134-144
[27] Wei C Z,Yu Q,Bai E,et al. Nitrogen deposition weakens plant-microbe interactions in grassland ecosystems[J]. Global Change Biology,2013,19(12):3688-3697
[28] Gregor W Y. Nematodes as soil indicators:functional and biodiversity aspects[J]. Biology and Fertility of Soil,2003,37(4):199-210

贝加尔针茅草原土壤线虫与微生物群落特征及其相互作用

Characteristics and Interaction of Soil Nematodes and Microbial Communities in Stipa Grandis Grassland

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