草地学报 ›› 2019, Vol. 27 ›› Issue (6): 1503-1510.DOI: 10.11733/j.issn.1007-0435.2019.06.006

• 研究论文 • 上一篇    下一篇

低温胁迫对不同早熟禾品种糖酵解代谢及其相关基因表达的影响

董文科1, 马祥2, 张玉娟1, 郭睿1, 张兆恒1, 张岩1, 马晖玲1   

  1. 1. 甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 甘肃省草业工程实验室, 中-美草地畜牧业可持续发展研究中心, 甘肃 兰州 730070;
    2. 青藏高原优良牧草种质资源利用重点实验室, 青海省畜牧兽医科学院, 青海 西宁 810016
  • 收稿日期:2019-08-06 修回日期:2019-10-02 出版日期:2019-12-15 发布日期:2019-12-31
  • 通讯作者: 马晖玲,E-mail:mahl@gsau.edu.cn
  • 作者简介:董文科(1990-),男,甘肃金昌人,博士研究生,主要从事牧草及草坪草种质资源育种研究,E-mail:3227542401@qq.com
  • 基金资助:
    青海省科技厅重点实验室发展专项"青海省青藏高原优良牧草种质资源利用重点实验室"(2017-ZJ-Y12)资助

Effects of Low-temperature Stress on Glycolysis Metabolism and Related Gene Expression of Different Poa pratensis Varieties

DONG Wen-ke1, MA Xiang2, ZHANG Yu-juan1, GUO Rui1, ZHANG Zhao-heng1, ZHANG Yan1, MA Hui-ling1   

  1. 1. College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-U. S. Center for Grassland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China;
    2. Key Laboratory of Superior Forage Germplasm in the Qinghai-Tibetan Plateau, Qinghai Academy of Animal Science and Veterinary Medicine, Xining, Qinghai Province 810016, China
  • Received:2019-08-06 Revised:2019-10-02 Online:2019-12-15 Published:2019-12-31

摘要: 为了探究青海扁茎早熟禾(Poa pratensis var.anceps cv.Qinghai,PQ)糖酵解代谢与其耐寒性的关系,以对温度敏感的草地早熟禾‘巴润’(Poa pratensis ‘Baron’,PB)为对照,在人工气候培养箱中模拟低温(0℃)胁迫,并测定2种材料的细胞膜伤害情况及与糖酵解途径相关的指标。结果表明:低温胁迫下,PQ的细胞膜损伤程度显著低于PB,表现出较强的抗寒性;同时,低温胁迫可引起PQ中可溶性糖、蔗糖、果糖和丙酮酸含量的上升,且显著高于PB;低温胁迫下,PQ的己糖激酶(Hexokinase,HxK)、磷酸果糖激酶(Phosphofructokinase,PFK)和丙酮酸激酶(Pyruvatekinase,PK)活性均显著提高,其中HxK和PFK对低温反应更敏感;PQ中上述3种关键酶对应编码的PpHxKPpPFKPpPK基因的相对表达量在低温胁迫下也明显被诱导上调,且上调幅度均高于PB。以上结果表明,PQ在低温胁迫下可以有效调控糖酵解代谢,进而促进糖类及丙酮酸的积累与分解,以缓解低温胁迫对其造成的伤害。

关键词: 低温胁迫, 青海扁茎早熟禾, 糖酵解, 基因表达

Abstract: In order to explore the relationship between glycolysis metabolism of Poa pratensis var. anceps cv. Qinghai (PQ) and cold resistance,we used Poa pratensis ‘Baron’ (PB) as a control and placed the two materials in an artificial climate incubator at 0℃ to simulate low temperature stress. At the same time,cell membrane damage and physiological indexes related to glycolysis pathway of the two materials were determined. The results showed that the damage degree of cell membrane of PQ was significantly lower than that of PB under low temperature stress,showing strong cold resistance. Moreover,low temperature stress could cause the increase of soluble sugar,sucrose,fructose and pyruvic acid content in PQ,and was significantly higher than that of PB. The activities of hexokinase,phosphofructokinase and pyruvatekinase of PQ were also significantly increased under low temperature stress. Hexokinase and phosphofructokinase were more sensitive to low temperature and increased rapidly at the early stage of stress. Similarly,the relative expression levels of PpHxK,PpPFK and PpPK genes were significantly increased after low temperature stress,and the up-regulation amplitude was higher than that of PB. In conclusion,PQ can effectively regulate glycolysis metabolism under low temperature stress,and then promote the accumulation and decomposition of carbohydrates and pyruvate acid to alleviate the damage caused by low temperature stress.

Key words: Low temperature stress, Poa pratensis var. anceps cv. Qinghai, Glycolysis, Gene expression

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